New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA
Mycoplasma hominis is a fastidious micro-organism causing genital and extragenital infections. We developed a specific real-time PCR that exhibits high sensitivity and low intrarun and interrun variabilities. When applied to clinical samples, this quantitative PCR allowed to confirm the role of M. h...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2010/317512 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832547331597139968 |
|---|---|
| author | Andres Pascual Katia Jaton Béatrice Ninet Jacques Bille Gilbert Greub |
| author_facet | Andres Pascual Katia Jaton Béatrice Ninet Jacques Bille Gilbert Greub |
| author_sort | Andres Pascual |
| collection | DOAJ |
| description | Mycoplasma hominis is a fastidious micro-organism causing genital and extragenital infections. We developed a specific real-time PCR that exhibits high sensitivity and low intrarun and interrun variabilities. When applied to clinical samples, this quantitative PCR allowed to confirm the role of M. hominis in three patients with severe extragenital infections. |
| format | Article |
| id | doaj-art-f727dd541bc4407e8bfb534a2d7fb204 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-f727dd541bc4407e8bfb534a2d7fb2042025-02-03T06:45:25ZengWileyInternational Journal of Microbiology1687-918X1687-91982010-01-01201010.1155/2010/317512317512New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNAAndres Pascual0Katia Jaton1Béatrice Ninet2Jacques Bille3Gilbert Greub4Infectious Diseases Service, Department of Internal Medicine, University Hospital Center and University of Lausanne, 1011 Lausanne, SwitzerlandInstitute of Microbiology, University Hospital Center and University of Lausanne, 1011 Lausanne, SwitzerlandLaboratory of Microbiology, University Hospital of Geneva, Geneva, SwitzerlandInfectious Diseases Service, Department of Internal Medicine, University Hospital Center and University of Lausanne, 1011 Lausanne, SwitzerlandInfectious Diseases Service, Department of Internal Medicine, University Hospital Center and University of Lausanne, 1011 Lausanne, SwitzerlandMycoplasma hominis is a fastidious micro-organism causing genital and extragenital infections. We developed a specific real-time PCR that exhibits high sensitivity and low intrarun and interrun variabilities. When applied to clinical samples, this quantitative PCR allowed to confirm the role of M. hominis in three patients with severe extragenital infections.http://dx.doi.org/10.1155/2010/317512 |
| spellingShingle | Andres Pascual Katia Jaton Béatrice Ninet Jacques Bille Gilbert Greub New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA International Journal of Microbiology |
| title | New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA |
| title_full | New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA |
| title_fullStr | New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA |
| title_full_unstemmed | New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA |
| title_short | New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA |
| title_sort | new diagnostic real time pcr for specific detection of mycoplasma hominis dna |
| url | http://dx.doi.org/10.1155/2010/317512 |
| work_keys_str_mv | AT andrespascual newdiagnosticrealtimepcrforspecificdetectionofmycoplasmahominisdna AT katiajaton newdiagnosticrealtimepcrforspecificdetectionofmycoplasmahominisdna AT beatriceninet newdiagnosticrealtimepcrforspecificdetectionofmycoplasmahominisdna AT jacquesbille newdiagnosticrealtimepcrforspecificdetectionofmycoplasmahominisdna AT gilbertgreub newdiagnosticrealtimepcrforspecificdetectionofmycoplasmahominisdna |