Donkey milk supplementation alleviates renal fibrosis of chronic kidney disease by enhancing anti-inflammatory ability
ABSTRACT: Donkey milk, rich in essential nutrients, is not only able to meet the nutritional needs of patients with chronic kidney disease (CKD) according to the National Kidney Foundation's Kidney Disease Outcomes Quality Initiative Clinical Practice Guideline for Nutrition in CKD, but also ha...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030224012591 |
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| author | Cui Huimin Wang Peng Chen Man Lin Hong Zhao Yuxin Pu Zhiyu Yang Jianjun |
| author_facet | Cui Huimin Wang Peng Chen Man Lin Hong Zhao Yuxin Pu Zhiyu Yang Jianjun |
| author_sort | Cui Huimin |
| collection | DOAJ |
| description | ABSTRACT: Donkey milk, rich in essential nutrients, is not only able to meet the nutritional needs of patients with chronic kidney disease (CKD) according to the National Kidney Foundation's Kidney Disease Outcomes Quality Initiative Clinical Practice Guideline for Nutrition in CKD, but also has strong anti-inflammatory properties. Inflammation is the main driving force of renal fibrosis, which directly determines renal function in CKD. However, the impact of donkey milk on CKD and the underlying mechanisms remain unclear. We hypothesized that donkey milk supplementation could enhance anti-inflammatory abilities in individuals with CKD, thereby inhibiting renal fibrosis and improving renal function. Therefore, we investigated the influence of a 6-wk treatment with donkey milk on unilateral ureteral ligation–induced CKD rats. The results demonstrated that the level of miR-223-3p was decreased in the kidneys of CKD rats compared with normal rats. However, donkey milk supplementation activated miR-223-3p, thereby inhibiting the expression of inhibitor of kappa-B kinase (IKKα) and nuclear factor kappa-B (NF-κB) proteins. Consequently, the expression of inflammatory factors such as monocyte chemotactic protein-1 (MCP-1) and IL-6 was suppressed, leading to reduced aggregations of macrophages in the kidney and decreased transforming growth factor β1 (TGF-β1) expression. Ultimately, renal fibrosis was inhibited, which was confirmed by decreased collagen type I α 1 (COL1A1) mRNA, thereby the renal function of CKD rats was improved. These results indicate that miR-223-3p may be another potential therapeutic target for CKD. Moreover, the contribution of donkey milk to alleviating the inflammatory response and renal fibrosis of CKD is obvious, indicating that donkey milk may be a new functional food for CKD patients to promote health. |
| format | Article |
| id | doaj-art-f71e9fd073784dc6bd8bd8c338d2fe56 |
| institution | Kabale University |
| issn | 0022-0302 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj-art-f71e9fd073784dc6bd8bd8c338d2fe562025-01-23T05:25:11ZengElsevierJournal of Dairy Science0022-03022025-02-01108211981210Donkey milk supplementation alleviates renal fibrosis of chronic kidney disease by enhancing anti-inflammatory abilityCui Huimin0Wang Peng1Chen Man2Lin Hong3Zhao Yuxin4Pu Zhiyu5Yang Jianjun6School of Public Health, Ningxia Medical University, Yinchuan 750004, China; Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan 750004, ChinaEmergency Center, Ningxia Medical University General Hospital, Yinchuan 750004, ChinaSchool of Public Health, Ningxia Medical University, Yinchuan 750004, China; Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan 750004, ChinaSchool of Public Health, Ningxia Medical University, Yinchuan 750004, China; Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan 750004, ChinaSchool of Public Health, Ningxia Medical University, Yinchuan 750004, China; Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan 750004, ChinaSchool of Public Health, Ningxia Medical University, Yinchuan 750004, China; Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan 750004, ChinaSchool of Public Health, Ningxia Medical University, Yinchuan 750004, China; Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan 750004, China; Corresponding authorABSTRACT: Donkey milk, rich in essential nutrients, is not only able to meet the nutritional needs of patients with chronic kidney disease (CKD) according to the National Kidney Foundation's Kidney Disease Outcomes Quality Initiative Clinical Practice Guideline for Nutrition in CKD, but also has strong anti-inflammatory properties. Inflammation is the main driving force of renal fibrosis, which directly determines renal function in CKD. However, the impact of donkey milk on CKD and the underlying mechanisms remain unclear. We hypothesized that donkey milk supplementation could enhance anti-inflammatory abilities in individuals with CKD, thereby inhibiting renal fibrosis and improving renal function. Therefore, we investigated the influence of a 6-wk treatment with donkey milk on unilateral ureteral ligation–induced CKD rats. The results demonstrated that the level of miR-223-3p was decreased in the kidneys of CKD rats compared with normal rats. However, donkey milk supplementation activated miR-223-3p, thereby inhibiting the expression of inhibitor of kappa-B kinase (IKKα) and nuclear factor kappa-B (NF-κB) proteins. Consequently, the expression of inflammatory factors such as monocyte chemotactic protein-1 (MCP-1) and IL-6 was suppressed, leading to reduced aggregations of macrophages in the kidney and decreased transforming growth factor β1 (TGF-β1) expression. Ultimately, renal fibrosis was inhibited, which was confirmed by decreased collagen type I α 1 (COL1A1) mRNA, thereby the renal function of CKD rats was improved. These results indicate that miR-223-3p may be another potential therapeutic target for CKD. Moreover, the contribution of donkey milk to alleviating the inflammatory response and renal fibrosis of CKD is obvious, indicating that donkey milk may be a new functional food for CKD patients to promote health.http://www.sciencedirect.com/science/article/pii/S0022030224012591chronic kidney diseasedonkey milkmiR-223-3pfibrosisinflammatory response |
| spellingShingle | Cui Huimin Wang Peng Chen Man Lin Hong Zhao Yuxin Pu Zhiyu Yang Jianjun Donkey milk supplementation alleviates renal fibrosis of chronic kidney disease by enhancing anti-inflammatory ability Journal of Dairy Science chronic kidney disease donkey milk miR-223-3p fibrosis inflammatory response |
| title | Donkey milk supplementation alleviates renal fibrosis of chronic kidney disease by enhancing anti-inflammatory ability |
| title_full | Donkey milk supplementation alleviates renal fibrosis of chronic kidney disease by enhancing anti-inflammatory ability |
| title_fullStr | Donkey milk supplementation alleviates renal fibrosis of chronic kidney disease by enhancing anti-inflammatory ability |
| title_full_unstemmed | Donkey milk supplementation alleviates renal fibrosis of chronic kidney disease by enhancing anti-inflammatory ability |
| title_short | Donkey milk supplementation alleviates renal fibrosis of chronic kidney disease by enhancing anti-inflammatory ability |
| title_sort | donkey milk supplementation alleviates renal fibrosis of chronic kidney disease by enhancing anti inflammatory ability |
| topic | chronic kidney disease donkey milk miR-223-3p fibrosis inflammatory response |
| url | http://www.sciencedirect.com/science/article/pii/S0022030224012591 |
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