Polypeptide Expression in Prostate Hyperplasia and Prostate Adenocarcinoma
Cells were collected from prostate hyperplasias (n=6) and prostate carcinomas (n=6) and subjected to two‐dimensional gel electrophoresis (2‐DE). The resulting polypeptide patterns were analysed with the PDQUEST computer software. Malignant tumors showed significant increases in the level of expressi...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2000-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2000/351963 |
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| _version_ | 1832562987953553408 |
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| author | Ayodele Alaiya Uwe Roblick Lars Egevad Adelaide Carlsson Bo Franzén Daniela Volz Sören Huwendiek Stig Linder Gert Auer |
| author_facet | Ayodele Alaiya Uwe Roblick Lars Egevad Adelaide Carlsson Bo Franzén Daniela Volz Sören Huwendiek Stig Linder Gert Auer |
| author_sort | Ayodele Alaiya |
| collection | DOAJ |
| description | Cells were collected from prostate hyperplasias (n=6) and prostate carcinomas (n=6) and subjected to two‐dimensional gel electrophoresis (2‐DE). The resulting polypeptide patterns were analysed with the PDQUEST computer software. Malignant tumors showed significant increases in the level of expression of proliferating cell nuclear antigen (PCNA), calreticulin, HSP 90 and pHSP 60, oncoprotein 18(v), elongation factor 2, glutathione‐S‐transferase π (GST‐π), superoxide dismutase and triose phosphate isomerase. In addition, decreases in the levels of tropomyosin‐1 and 2 and cytokeratin 18 were observed in prostate carcinomas compared to prostate hyperplasias. This pattern of alterations is similar to that observed in other carcinomas in our previous studies. All malignant tumors showed simultaneous alterations in 5 or more of 9 markers studied, whereas only one case of benign hyperplasia showed alterations in 5 markers. The EST‐data base for prostate tumors available from NCI (CGAP) was searched for the expression of the mRNAs corresponding to proteins identified in our gels. Large differences in the relative expression of mRNAs and proteins were observed. Our data show alterations in the pattern of polypeptide expression in prostate carcinomas which are similar to those observed in other carcinomas. |
| format | Article |
| id | doaj-art-f703c4ae00e5454b8054940be80f1a87 |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-f703c4ae00e5454b8054940be80f1a872025-02-03T01:21:22ZengWileyAnalytical Cellular Pathology0921-89121878-36512000-01-012111910.1155/2000/351963Polypeptide Expression in Prostate Hyperplasia and Prostate AdenocarcinomaAyodele Alaiya0Uwe Roblick1Lars Egevad2Adelaide Carlsson3Bo Franzén4Daniela Volz5Sören Huwendiek6Stig Linder7Gert Auer8Unit of Cancer Proteomics, Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska Institute and Hospital, S‐171 76 Stockholm, SwedenUnit of Cancer Proteomics, Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska Institute and Hospital, S‐171 76 Stockholm, SwedenDepartment of Pathology and Cytology, Radiumhemmet, Karolinska Hospital, S‐171 76 Stockholm, SwedenSabbatsbergs Hospital, Olivecronas väg 1, S‐113 24 Stockholm, SwedenUnit of Cancer Proteomics, Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska Institute and Hospital, S‐171 76 Stockholm, SwedenDepartment of Urology, Karolinska Institute and Hospital, S‐171 76 Stockholm, SwedenUnit of Cancer Proteomics, Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska Institute and Hospital, S‐171 76 Stockholm, SwedenRadiumhemmets Research Laboratory, Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska Institute and Hospital, S‐171 76 Stockholm, SwedenUnit of Cancer Proteomics, Department of Oncology and Pathology, Cancer Center Karolinska, Karolinska Institute and Hospital, S‐171 76 Stockholm, SwedenCells were collected from prostate hyperplasias (n=6) and prostate carcinomas (n=6) and subjected to two‐dimensional gel electrophoresis (2‐DE). The resulting polypeptide patterns were analysed with the PDQUEST computer software. Malignant tumors showed significant increases in the level of expression of proliferating cell nuclear antigen (PCNA), calreticulin, HSP 90 and pHSP 60, oncoprotein 18(v), elongation factor 2, glutathione‐S‐transferase π (GST‐π), superoxide dismutase and triose phosphate isomerase. In addition, decreases in the levels of tropomyosin‐1 and 2 and cytokeratin 18 were observed in prostate carcinomas compared to prostate hyperplasias. This pattern of alterations is similar to that observed in other carcinomas in our previous studies. All malignant tumors showed simultaneous alterations in 5 or more of 9 markers studied, whereas only one case of benign hyperplasia showed alterations in 5 markers. The EST‐data base for prostate tumors available from NCI (CGAP) was searched for the expression of the mRNAs corresponding to proteins identified in our gels. Large differences in the relative expression of mRNAs and proteins were observed. Our data show alterations in the pattern of polypeptide expression in prostate carcinomas which are similar to those observed in other carcinomas.http://dx.doi.org/10.1155/2000/351963 |
| spellingShingle | Ayodele Alaiya Uwe Roblick Lars Egevad Adelaide Carlsson Bo Franzén Daniela Volz Sören Huwendiek Stig Linder Gert Auer Polypeptide Expression in Prostate Hyperplasia and Prostate Adenocarcinoma Analytical Cellular Pathology |
| title | Polypeptide Expression in Prostate Hyperplasia and Prostate Adenocarcinoma |
| title_full | Polypeptide Expression in Prostate Hyperplasia and Prostate Adenocarcinoma |
| title_fullStr | Polypeptide Expression in Prostate Hyperplasia and Prostate Adenocarcinoma |
| title_full_unstemmed | Polypeptide Expression in Prostate Hyperplasia and Prostate Adenocarcinoma |
| title_short | Polypeptide Expression in Prostate Hyperplasia and Prostate Adenocarcinoma |
| title_sort | polypeptide expression in prostate hyperplasia and prostate adenocarcinoma |
| url | http://dx.doi.org/10.1155/2000/351963 |
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