Bio-efficacy of Nanosilicon in Regulating Oxidative Activity to Control Rice Seedlings Rot Disease Caused by Burkholderia glumae

Bacterial panicle blight and seedling rot diseases in rice plants (Oryza sativa L.) are caused by the pathogenic bacterial Burkholderia glumae. The nanosilicon treatment is gaining attraction but its effectiveness towards B. glumae infection in rice seedlings through regulating enzymatic activities...

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Main Authors: Tamilselvan R. Govinda Rajoo, Muhamad Syazlie Che Ibrahim, Aziz Ahmad, Lee Chuen Ng
Format: Article
Language:English
Published: Hanrimwon Publishing Company 2025-04-01
Series:The Plant Pathology Journal
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Online Access:http://ppjonline.org/upload/pdf/PPJ-OA-08-2024-0123.pdf
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author Tamilselvan R. Govinda Rajoo
Muhamad Syazlie Che Ibrahim
Aziz Ahmad
Lee Chuen Ng
author_facet Tamilselvan R. Govinda Rajoo
Muhamad Syazlie Che Ibrahim
Aziz Ahmad
Lee Chuen Ng
author_sort Tamilselvan R. Govinda Rajoo
collection DOAJ
description Bacterial panicle blight and seedling rot diseases in rice plants (Oryza sativa L.) are caused by the pathogenic bacterial Burkholderia glumae. The nanosilicon treatment is gaining attraction but its effectiveness towards B. glumae infection in rice seedlings through regulating enzymatic activities remains largely unexplored. This study aimed to evaluate the bio-efficacy of nanosilicon in controlling seedling rot disease through regulation of peroxidase and polyphenol oxidase enzymes after challenge infected with B. glumae in rice variety MR297 and PadiU Putra. Nanosilicon was applied as seed priming in germination testing at 0, 300, 600, and 900 ppm on both rice varieties before B. glumae inoculation. Both rice seed varieties primed with nanosilicon at 600 ppm exhibited a significant increase in seedling germination performances over control. The rice seedling of MR297 was more responsive to nanosilicon at 600 ppm with only 17.78% of disease severity index over 26.67% in PadiU Putra and was therefore selected for the enzymatic activity screening. The results showed that the foliar spray of nanosilicon rice plants (MR297) significantly increased both peroxidase (POX) at 24 h and polyphenol oxidase (PPO) at 48 h after B. glumae inoculation with 20.44/min/g and 7.46/g activities, respectively. In addition, the plant growth performances were significantly increased compared with control under the same treatment. This demonstrates nanosilicon’s potential to control rice seedling rot disease by regulating POX and PPO activities and hence promote plant growth. The application of nanosilicon is an environmentally friendly approach for controlling B. glumae infection at the early rice growing stage.
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2093-9280
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spelling doaj-art-f6f1a4bc9f2f4e00b45076d40d14ffb82025-08-20T02:32:08ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802025-04-0141215316610.5423/PPJ.OA.08.2024.01232497Bio-efficacy of Nanosilicon in Regulating Oxidative Activity to Control Rice Seedlings Rot Disease Caused by Burkholderia glumaeTamilselvan R. Govinda Rajoo0Muhamad Syazlie Che Ibrahim1Aziz Ahmad2Lee Chuen Ng3 Research Interest Group of Resource Sustainability (Bio-interaction and Crop Health), Laboratory of Pest, Disease and Microbial Biotechnology (LAPDiM), Faculty of Fisheries and Food Science (FFFS), Universiti Malaysia Terengganu (UMT), 21030 Kuala Nerus, Terengganu, Malaysia Research Interest Group of Resource Sustainability (Bio-interaction and Crop Health), Laboratory of Pest, Disease and Microbial Biotechnology (LAPDiM), Faculty of Fisheries and Food Science (FFFS), Universiti Malaysia Terengganu (UMT), 21030 Kuala Nerus, Terengganu, Malaysia Biological Security and Sustainability Research Group, Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia Research Interest Group of Resource Sustainability (Bio-interaction and Crop Health), Laboratory of Pest, Disease and Microbial Biotechnology (LAPDiM), Faculty of Fisheries and Food Science (FFFS), Universiti Malaysia Terengganu (UMT), 21030 Kuala Nerus, Terengganu, MalaysiaBacterial panicle blight and seedling rot diseases in rice plants (Oryza sativa L.) are caused by the pathogenic bacterial Burkholderia glumae. The nanosilicon treatment is gaining attraction but its effectiveness towards B. glumae infection in rice seedlings through regulating enzymatic activities remains largely unexplored. This study aimed to evaluate the bio-efficacy of nanosilicon in controlling seedling rot disease through regulation of peroxidase and polyphenol oxidase enzymes after challenge infected with B. glumae in rice variety MR297 and PadiU Putra. Nanosilicon was applied as seed priming in germination testing at 0, 300, 600, and 900 ppm on both rice varieties before B. glumae inoculation. Both rice seed varieties primed with nanosilicon at 600 ppm exhibited a significant increase in seedling germination performances over control. The rice seedling of MR297 was more responsive to nanosilicon at 600 ppm with only 17.78% of disease severity index over 26.67% in PadiU Putra and was therefore selected for the enzymatic activity screening. The results showed that the foliar spray of nanosilicon rice plants (MR297) significantly increased both peroxidase (POX) at 24 h and polyphenol oxidase (PPO) at 48 h after B. glumae inoculation with 20.44/min/g and 7.46/g activities, respectively. In addition, the plant growth performances were significantly increased compared with control under the same treatment. This demonstrates nanosilicon’s potential to control rice seedling rot disease by regulating POX and PPO activities and hence promote plant growth. The application of nanosilicon is an environmentally friendly approach for controlling B. glumae infection at the early rice growing stage.http://ppjonline.org/upload/pdf/PPJ-OA-08-2024-0123.pdfenzymatic activitynanosilicon
spellingShingle Tamilselvan R. Govinda Rajoo
Muhamad Syazlie Che Ibrahim
Aziz Ahmad
Lee Chuen Ng
Bio-efficacy of Nanosilicon in Regulating Oxidative Activity to Control Rice Seedlings Rot Disease Caused by Burkholderia glumae
The Plant Pathology Journal
enzymatic activity
nanosilicon
title Bio-efficacy of Nanosilicon in Regulating Oxidative Activity to Control Rice Seedlings Rot Disease Caused by Burkholderia glumae
title_full Bio-efficacy of Nanosilicon in Regulating Oxidative Activity to Control Rice Seedlings Rot Disease Caused by Burkholderia glumae
title_fullStr Bio-efficacy of Nanosilicon in Regulating Oxidative Activity to Control Rice Seedlings Rot Disease Caused by Burkholderia glumae
title_full_unstemmed Bio-efficacy of Nanosilicon in Regulating Oxidative Activity to Control Rice Seedlings Rot Disease Caused by Burkholderia glumae
title_short Bio-efficacy of Nanosilicon in Regulating Oxidative Activity to Control Rice Seedlings Rot Disease Caused by Burkholderia glumae
title_sort bio efficacy of nanosilicon in regulating oxidative activity to control rice seedlings rot disease caused by burkholderia glumae
topic enzymatic activity
nanosilicon
url http://ppjonline.org/upload/pdf/PPJ-OA-08-2024-0123.pdf
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