Establishment of the first marine mollusk cell line from scallop (Chlamys farreri) trochophore
Cell lines are indispensable tools in biological studies. Decades of attempts have failed to establish a cell line in marine mollusks, which contribute to 70 % of the mariculture production in China. In the present study, FBS, scallop serum, scallop ovarian extracts, and FeCl3 were added to the L-15...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425000122 |
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| author | Zhenkui Qin Aichang Ji Meng Yan Danwen Liu Xixi Li Xiaoli Hu Zhifeng Zhang |
| author_facet | Zhenkui Qin Aichang Ji Meng Yan Danwen Liu Xixi Li Xiaoli Hu Zhifeng Zhang |
| author_sort | Zhenkui Qin |
| collection | DOAJ |
| description | Cell lines are indispensable tools in biological studies. Decades of attempts have failed to establish a cell line in marine mollusks, which contribute to 70 % of the mariculture production in China. In the present study, FBS, scallop serum, scallop ovarian extracts, and FeCl3 were added to the L-15 basic medium to develop an optimized culture medium (L15-CFIIIFe) for culturing trochophore larval cells of Chlamys farreri, an important mariculture scallop in China. C. farreri trochophore larval (CfT) cells exhibited proliferative activity, and a CfT cell line was successfully established, which was passaged for 155 generations in vitro. The origin of the CfT cell line was verified as C. farreri using internal transcribed spacer (ITS) and 18 s rRNA gene amplification, genomic in situ hybridization (GISH), and karyotype analysis. The CfT cell line was a mixture of cells of three sizes (5–7, 9–12, and 18–22 μm), with a population doubling time of 75.6 h. Immunofluorescence detection of KLF4, c-MYC, MYOSIN, and 5-HT indicated that CfT cells may possess a certain degree of pluripotency. Four gene transfer methods were tested in the CfT cell line; however, the transfer efficiency (0.5 %-1 %) requires further optimization. We successfully established the first marine mollusk cell line, providing a valuable cellular tool for mollusk studies. |
| format | Article |
| id | doaj-art-f6d69820af4a4477a0452dedb5bfe46f |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-f6d69820af4a4477a0452dedb5bfe46f2025-02-06T05:12:21ZengElsevierAquaculture Reports2352-51342025-03-0140102626Establishment of the first marine mollusk cell line from scallop (Chlamys farreri) trochophoreZhenkui Qin0Aichang Ji1Meng Yan2Danwen Liu3Xixi Li4Xiaoli Hu5Zhifeng Zhang6Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Shandong Provincial Key Laboratory of Metabolic Diseases, The Affiliated Hospital of Qingdao University, Qingdao, ChinaMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; State Key Laboratory of Marine Pollution, City University of Hong Kong, 999077, Hong KongMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Corresponding author.Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya 572000, China; Corresponding author at: Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.Cell lines are indispensable tools in biological studies. Decades of attempts have failed to establish a cell line in marine mollusks, which contribute to 70 % of the mariculture production in China. In the present study, FBS, scallop serum, scallop ovarian extracts, and FeCl3 were added to the L-15 basic medium to develop an optimized culture medium (L15-CFIIIFe) for culturing trochophore larval cells of Chlamys farreri, an important mariculture scallop in China. C. farreri trochophore larval (CfT) cells exhibited proliferative activity, and a CfT cell line was successfully established, which was passaged for 155 generations in vitro. The origin of the CfT cell line was verified as C. farreri using internal transcribed spacer (ITS) and 18 s rRNA gene amplification, genomic in situ hybridization (GISH), and karyotype analysis. The CfT cell line was a mixture of cells of three sizes (5–7, 9–12, and 18–22 μm), with a population doubling time of 75.6 h. Immunofluorescence detection of KLF4, c-MYC, MYOSIN, and 5-HT indicated that CfT cells may possess a certain degree of pluripotency. Four gene transfer methods were tested in the CfT cell line; however, the transfer efficiency (0.5 %-1 %) requires further optimization. We successfully established the first marine mollusk cell line, providing a valuable cellular tool for mollusk studies.http://www.sciencedirect.com/science/article/pii/S2352513425000122Chlamys farreriTrochophore larvaCell culture mediumCell line |
| spellingShingle | Zhenkui Qin Aichang Ji Meng Yan Danwen Liu Xixi Li Xiaoli Hu Zhifeng Zhang Establishment of the first marine mollusk cell line from scallop (Chlamys farreri) trochophore Aquaculture Reports Chlamys farreri Trochophore larva Cell culture medium Cell line |
| title | Establishment of the first marine mollusk cell line from scallop (Chlamys farreri) trochophore |
| title_full | Establishment of the first marine mollusk cell line from scallop (Chlamys farreri) trochophore |
| title_fullStr | Establishment of the first marine mollusk cell line from scallop (Chlamys farreri) trochophore |
| title_full_unstemmed | Establishment of the first marine mollusk cell line from scallop (Chlamys farreri) trochophore |
| title_short | Establishment of the first marine mollusk cell line from scallop (Chlamys farreri) trochophore |
| title_sort | establishment of the first marine mollusk cell line from scallop chlamys farreri trochophore |
| topic | Chlamys farreri Trochophore larva Cell culture medium Cell line |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425000122 |
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