Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor
Skin represents an attractive target for DNA vaccine delivery because of its natural richness in APCs, whose targeting may potentiate the effect of vaccination. Nevertheless, intramuscular electroporation is the most common delivery method for ECTM vaccination. In this study we assessed whether intr...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/159145 |
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| author | Alessia Lamolinara Lorenzo Stramucci Albana Hysi Manuela Iezzi Cristina Marchini Marianna Mariotti Augusto Amici Claudia Curcio |
| author_facet | Alessia Lamolinara Lorenzo Stramucci Albana Hysi Manuela Iezzi Cristina Marchini Marianna Mariotti Augusto Amici Claudia Curcio |
| author_sort | Alessia Lamolinara |
| collection | DOAJ |
| description | Skin represents an attractive target for DNA vaccine delivery because of its natural richness in APCs, whose targeting may potentiate the effect of vaccination. Nevertheless, intramuscular electroporation is the most common delivery method for ECTM vaccination. In this study we assessed whether intradermal administration could deliver the vaccine into different cell types and we analyzed the evolution of tissue infiltrate elicited by the vaccination protocol. Intradermal electroporation (EP) vaccination resulted in transfection of different skin layers, as well as mononuclear cells. Additionally, we observed a marked recruitment of reactive infiltrates mainly 6–24 hours after treatment and inflammatory cells included CD11c+.
Moreover, we tested the efficacy of intradermal vaccination against Her2/neu antigen in cellular and humoral response induction and consequent protection from a Her2/neu tumor challenge in Her2/neu nontolerant and tolerant mice. A significant delay in transplantable tumor onset was observed in both BALB/c (p≤0,0003) and BALB-neuT mice (p=0,003). Moreover, BALB-neuT mice displayed slow tumor growth as compared to control group (p<0,0016). In addition, while in vivo cytotoxic response was observed only in BALB/c mice, a significant antibody response was achieved in both mouse models. Our results identify intradermal EP vaccination as a promising method for delivering Her2/neu DNA vaccine. |
| format | Article |
| id | doaj-art-f6c185c1057d486abb29ff697a116dcb |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-f6c185c1057d486abb29ff697a116dcb2025-02-03T05:58:37ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/159145159145Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu TumorAlessia Lamolinara0Lorenzo Stramucci1Albana Hysi2Manuela Iezzi3Cristina Marchini4Marianna Mariotti5Augusto Amici6Claudia Curcio7Aging Research Center, G. d’Annunzio University, 66100 Chieti, ItalyAging Research Center, G. d’Annunzio University, 66100 Chieti, ItalyAging Research Center, G. d’Annunzio University, 66100 Chieti, ItalyAging Research Center, G. d’Annunzio University, 66100 Chieti, ItalyDepartment of Bioscience and Biotechnology, University of Camerino, Camerino, 63100 Macerata, ItalyAging Research Center, G. d’Annunzio University, 66100 Chieti, ItalyDepartment of Bioscience and Biotechnology, University of Camerino, Camerino, 63100 Macerata, ItalyAging Research Center, G. d’Annunzio University, 66100 Chieti, ItalySkin represents an attractive target for DNA vaccine delivery because of its natural richness in APCs, whose targeting may potentiate the effect of vaccination. Nevertheless, intramuscular electroporation is the most common delivery method for ECTM vaccination. In this study we assessed whether intradermal administration could deliver the vaccine into different cell types and we analyzed the evolution of tissue infiltrate elicited by the vaccination protocol. Intradermal electroporation (EP) vaccination resulted in transfection of different skin layers, as well as mononuclear cells. Additionally, we observed a marked recruitment of reactive infiltrates mainly 6–24 hours after treatment and inflammatory cells included CD11c+. Moreover, we tested the efficacy of intradermal vaccination against Her2/neu antigen in cellular and humoral response induction and consequent protection from a Her2/neu tumor challenge in Her2/neu nontolerant and tolerant mice. A significant delay in transplantable tumor onset was observed in both BALB/c (p≤0,0003) and BALB-neuT mice (p=0,003). Moreover, BALB-neuT mice displayed slow tumor growth as compared to control group (p<0,0016). In addition, while in vivo cytotoxic response was observed only in BALB/c mice, a significant antibody response was achieved in both mouse models. Our results identify intradermal EP vaccination as a promising method for delivering Her2/neu DNA vaccine.http://dx.doi.org/10.1155/2015/159145 |
| spellingShingle | Alessia Lamolinara Lorenzo Stramucci Albana Hysi Manuela Iezzi Cristina Marchini Marianna Mariotti Augusto Amici Claudia Curcio Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor Journal of Immunology Research |
| title | Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor |
| title_full | Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor |
| title_fullStr | Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor |
| title_full_unstemmed | Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor |
| title_short | Intradermal DNA Electroporation Induces Cellular and Humoral Immune Response and Confers Protection against HER2/neu Tumor |
| title_sort | intradermal dna electroporation induces cellular and humoral immune response and confers protection against her2 neu tumor |
| url | http://dx.doi.org/10.1155/2015/159145 |
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