Genetic Variation of Lentil Landraces Across Different Altitudes in Azerbaijan
Lentil is mainly grown in rainfed systems and thus plays an important role in sustainable agriculture, especially in high mountain landscapes. The aim of this study was to assess the genetic diversity of 50 lentil accessions, including landraces from high mountain regions of Azerbaijan as well as wi...
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EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/02/bioconf_mblc2024_01010.pdf |
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| author | Babayeva Sevda Shikhaliyeva Kamila Qurbanova Qamar Hajiyeva Sabina Izzatullayeva Vusala Rustamov Khanbala Abbasov Mehraj |
| author_facet | Babayeva Sevda Shikhaliyeva Kamila Qurbanova Qamar Hajiyeva Sabina Izzatullayeva Vusala Rustamov Khanbala Abbasov Mehraj |
| author_sort | Babayeva Sevda |
| collection | DOAJ |
| description | Lentil is mainly grown in rainfed systems and thus plays an important role in sustainable agriculture, especially in high mountain landscapes. The aim of this study was to assess the genetic diversity of 50 lentil accessions, including landraces from high mountain regions of Azerbaijan as well as wild lentil species, using SSR markers. DNA was extracted from leaves, amplified by PCR and separated by polyacrylamide gel electrophoresis. Statistical analysis included dendrogram construction and principal coordinate analysis (PCoA). A total of 26 alleles were identified, with an average of 8.6. Primers SSR 156 and SSR 323 showed transferability between all studied species/subspecies within the genus Lens. The genetic diversity index for each primer ranged from 0.704 to 0.776, with a mean value of 0.735. Accessions from higher altitudes such as Ismayilli and Nakhchivan contributed significantly to the observed genetic variation. The dendrogram revealed four clusters in which 71.4% of the accessions were distinguishable. Lens culinary subsp. odemensis formed a distinct subcluster due to its high genetic distance. PCoA analysis revealed a dispersed sample distribution, with the first five axes accounting for 69.9% of the total variation. The study revealed significant genetic diversity among lentil accessions in Azerbaijan, including highland regions. These results highlight the need for conservation and will support future breeding and sustainable agriculture. |
| format | Article |
| id | doaj-art-f6bd1564e93e4d8e965d31a30d0a5cef |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-f6bd1564e93e4d8e965d31a30d0a5cef2025-02-05T10:42:41ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011510101010.1051/bioconf/202515101010bioconf_mblc2024_01010Genetic Variation of Lentil Landraces Across Different Altitudes in AzerbaijanBabayeva Sevda0Shikhaliyeva Kamila1Qurbanova Qamar2Hajiyeva Sabina3Izzatullayeva Vusala4Rustamov Khanbala5Abbasov Mehraj6Genetic Resources Institute of AR SEM, Molecular Genetics and Genomics departmentGenetic Resources Institute of AR SEM, Molecular Genetics and Genomics departmentGenetic Resources Institute of AR SEM, Molecular Genetics and Genomics departmentGenetic Resources Institute of AR SEM, Molecular Genetics and Genomics departmentGenetic Resources Institute of AR SEM, Molecular Genetics and Genomics departmentGenetic Resources Institute of AR SEM, Molecular Genetics and Genomics departmentGenetic Resources Institute of AR SEM, Molecular Genetics and Genomics departmentLentil is mainly grown in rainfed systems and thus plays an important role in sustainable agriculture, especially in high mountain landscapes. The aim of this study was to assess the genetic diversity of 50 lentil accessions, including landraces from high mountain regions of Azerbaijan as well as wild lentil species, using SSR markers. DNA was extracted from leaves, amplified by PCR and separated by polyacrylamide gel electrophoresis. Statistical analysis included dendrogram construction and principal coordinate analysis (PCoA). A total of 26 alleles were identified, with an average of 8.6. Primers SSR 156 and SSR 323 showed transferability between all studied species/subspecies within the genus Lens. The genetic diversity index for each primer ranged from 0.704 to 0.776, with a mean value of 0.735. Accessions from higher altitudes such as Ismayilli and Nakhchivan contributed significantly to the observed genetic variation. The dendrogram revealed four clusters in which 71.4% of the accessions were distinguishable. Lens culinary subsp. odemensis formed a distinct subcluster due to its high genetic distance. PCoA analysis revealed a dispersed sample distribution, with the first five axes accounting for 69.9% of the total variation. The study revealed significant genetic diversity among lentil accessions in Azerbaijan, including highland regions. These results highlight the need for conservation and will support future breeding and sustainable agriculture.https://www.bio-conferences.org/articles/bioconf/pdf/2025/02/bioconf_mblc2024_01010.pdf |
| spellingShingle | Babayeva Sevda Shikhaliyeva Kamila Qurbanova Qamar Hajiyeva Sabina Izzatullayeva Vusala Rustamov Khanbala Abbasov Mehraj Genetic Variation of Lentil Landraces Across Different Altitudes in Azerbaijan BIO Web of Conferences |
| title | Genetic Variation of Lentil Landraces Across Different Altitudes in Azerbaijan |
| title_full | Genetic Variation of Lentil Landraces Across Different Altitudes in Azerbaijan |
| title_fullStr | Genetic Variation of Lentil Landraces Across Different Altitudes in Azerbaijan |
| title_full_unstemmed | Genetic Variation of Lentil Landraces Across Different Altitudes in Azerbaijan |
| title_short | Genetic Variation of Lentil Landraces Across Different Altitudes in Azerbaijan |
| title_sort | genetic variation of lentil landraces across different altitudes in azerbaijan |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/02/bioconf_mblc2024_01010.pdf |
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