Purification of Anthocyanins with o-Dihydroxy Arrangement by Sorption in Cationic Resins Charged with Fe(III)
In the present work, a new purification method of anthocyanins with o-dihydroxy arrangement is proposed. This method is based on a ligand-exchange mechanism, using a cationic exchange resin loaded with metallic ions in order to increase the affinity of the resin to the anthocyanin(s) with o-dihydrox...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/367236 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563534547910656 |
|---|---|
| author | Araceli Castañeda-Ovando Carlos Andrés Galán-Vidal Elizabeth Contreras-López Ma. Elena Páez-Hernández |
| author_facet | Araceli Castañeda-Ovando Carlos Andrés Galán-Vidal Elizabeth Contreras-López Ma. Elena Páez-Hernández |
| author_sort | Araceli Castañeda-Ovando |
| collection | DOAJ |
| description | In the present work, a new purification method of anthocyanins with o-dihydroxy arrangement is proposed. This method is based on a ligand-exchange mechanism, using a cationic exchange resin loaded with metallic ions in order to increase the affinity of the resin to the anthocyanin(s) with o-dihydroxy arrangement. This method was used to purify the main anthocyanin (cyanidin-3-glucoside; Cy-3-glc) from the anthocyanic methanolic extract of blue corn. The best sorption result was using Fe(III) in its ion form. The purification procedure begins with the formation of a metal-anthocyanin complex (Cy-3-glc-Fe) which was optimal at pH 5, followed by a NaOH 0.1 M elution process in order to eliminate anthocyanins without o-dihydroxy arrangement, sugars, and organic acids. Finally, the pure anthocyanin is obtained by adding HCl 0.1 M which breaks the metal-anthocyanin complex. |
| format | Article |
| id | doaj-art-f6ad38f2d3c54261bdf8c3b5dcddd6ac |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-f6ad38f2d3c54261bdf8c3b5dcddd6ac2025-02-03T01:20:05ZengWileyJournal of Chemistry2090-90632090-90712014-01-01201410.1155/2014/367236367236Purification of Anthocyanins with o-Dihydroxy Arrangement by Sorption in Cationic Resins Charged with Fe(III)Araceli Castañeda-Ovando0Carlos Andrés Galán-Vidal1Elizabeth Contreras-López2Ma. Elena Páez-Hernández3Academic Area of Chemistry, Autonomous University of Hidalgo State, 42184 Mineral de la Reforma, HGO, MexicoAcademic Area of Chemistry, Autonomous University of Hidalgo State, 42184 Mineral de la Reforma, HGO, MexicoAcademic Area of Chemistry, Autonomous University of Hidalgo State, 42184 Mineral de la Reforma, HGO, MexicoAcademic Area of Chemistry, Autonomous University of Hidalgo State, 42184 Mineral de la Reforma, HGO, MexicoIn the present work, a new purification method of anthocyanins with o-dihydroxy arrangement is proposed. This method is based on a ligand-exchange mechanism, using a cationic exchange resin loaded with metallic ions in order to increase the affinity of the resin to the anthocyanin(s) with o-dihydroxy arrangement. This method was used to purify the main anthocyanin (cyanidin-3-glucoside; Cy-3-glc) from the anthocyanic methanolic extract of blue corn. The best sorption result was using Fe(III) in its ion form. The purification procedure begins with the formation of a metal-anthocyanin complex (Cy-3-glc-Fe) which was optimal at pH 5, followed by a NaOH 0.1 M elution process in order to eliminate anthocyanins without o-dihydroxy arrangement, sugars, and organic acids. Finally, the pure anthocyanin is obtained by adding HCl 0.1 M which breaks the metal-anthocyanin complex.http://dx.doi.org/10.1155/2014/367236 |
| spellingShingle | Araceli Castañeda-Ovando Carlos Andrés Galán-Vidal Elizabeth Contreras-López Ma. Elena Páez-Hernández Purification of Anthocyanins with o-Dihydroxy Arrangement by Sorption in Cationic Resins Charged with Fe(III) Journal of Chemistry |
| title | Purification of Anthocyanins with o-Dihydroxy Arrangement by Sorption in Cationic Resins Charged with Fe(III) |
| title_full | Purification of Anthocyanins with o-Dihydroxy Arrangement by Sorption in Cationic Resins Charged with Fe(III) |
| title_fullStr | Purification of Anthocyanins with o-Dihydroxy Arrangement by Sorption in Cationic Resins Charged with Fe(III) |
| title_full_unstemmed | Purification of Anthocyanins with o-Dihydroxy Arrangement by Sorption in Cationic Resins Charged with Fe(III) |
| title_short | Purification of Anthocyanins with o-Dihydroxy Arrangement by Sorption in Cationic Resins Charged with Fe(III) |
| title_sort | purification of anthocyanins with o dihydroxy arrangement by sorption in cationic resins charged with fe iii |
| url | http://dx.doi.org/10.1155/2014/367236 |
| work_keys_str_mv | AT aracelicastanedaovando purificationofanthocyaninswithodihydroxyarrangementbysorptionincationicresinschargedwithfeiii AT carlosandresgalanvidal purificationofanthocyaninswithodihydroxyarrangementbysorptionincationicresinschargedwithfeiii AT elizabethcontreraslopez purificationofanthocyaninswithodihydroxyarrangementbysorptionincationicresinschargedwithfeiii AT maelenapaezhernandez purificationofanthocyaninswithodihydroxyarrangementbysorptionincationicresinschargedwithfeiii |