Integrated metabolomic and transcriptomic analysis of anthocyanin metabolism in wheat pericarp
Abstract Background Wheat seeds display different colors due to the types and contents of anthocyanins, which is closely related to anthocyanin metabolism. In this study, a transcriptomic and metabolomic analysis between white and purple color wheat pericarp aimed to explore some key genes and metab...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12863-024-01294-y |
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| author | Jiao Wang Lei Sun Bo Jiao Pu Zhao Tianyun Xu Sa Gu Chenmin Huo Jianzhou Pang Shuo Zhou |
| author_facet | Jiao Wang Lei Sun Bo Jiao Pu Zhao Tianyun Xu Sa Gu Chenmin Huo Jianzhou Pang Shuo Zhou |
| author_sort | Jiao Wang |
| collection | DOAJ |
| description | Abstract Background Wheat seeds display different colors due to the types and contents of anthocyanins, which is closely related to anthocyanin metabolism. In this study, a transcriptomic and metabolomic analysis between white and purple color wheat pericarp aimed to explore some key genes and metabolites involved in anthocyanin metabolism. Results Two wheat cultivars, a white seed cultivar Shiluan02-1 and purple seed cultivar Hengzi151 were used to identify the variations in differentially expressed genes (DEGs) and differentially accumulated flavonoids (DAFs). Based on metabolomic data, 314 metabolites and 191 DAFs were identified. Chalcone, flavonol, pro-anthocyanidin and anthocyanidin were the most differentially accumulated flavonoid compounds in Hengzi151. 2610 up-regulated and 2668 down-regulated DEGs were identified according to transcriptomic data. The results showed that some structural genes in anthocyanin synthesis pathway were prominently activated in Hengzi151, such as PAL, CAD, CHS and so on. Transcription factors (TFs) of MYB, bHLH, WD40 and some other TFs probably involved in regulating anthocyanin biosynthesis were identified. Some genes from hormone synthetic and signaling pathways that may participate in regulating anthocyanin biosynthesis also have been identified. Conclusions Our results provide valuable information on the candidate genes and metabolites involved in the anthocyanin metabolism in wheat pericarp. |
| format | Article |
| id | doaj-art-f6560f1ad8fc4510848c60e3f996595d |
| institution | Kabale University |
| issn | 2730-6844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomic Data |
| spelling | doaj-art-f6560f1ad8fc4510848c60e3f996595d2025-01-19T12:40:31ZengBMCBMC Genomic Data2730-68442025-01-0126111810.1186/s12863-024-01294-yIntegrated metabolomic and transcriptomic analysis of anthocyanin metabolism in wheat pericarpJiao Wang0Lei Sun1Bo Jiao2Pu Zhao3Tianyun Xu4Sa Gu5Chenmin Huo6Jianzhou Pang7Shuo Zhou8Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences/Hebei Key Laboratory of Plant Genetic EngineeringInstitute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences/Hebei Key Laboratory of Plant Genetic EngineeringInstitute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences/Hebei Key Laboratory of Plant Genetic EngineeringInstitute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences/Hebei Key Laboratory of Plant Genetic EngineeringInstitute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences/Hebei Key Laboratory of Plant Genetic EngineeringInstitute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences/Hebei Key Laboratory of Plant Genetic EngineeringHebei University of Economics and BusinessDryland Farming Institute of Hebei Academy of Agricultural and Forestry Sciences/Key Laboratory of Crop Drought Tolerance Research of Hebei ProvinceInstitute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences/Hebei Key Laboratory of Plant Genetic EngineeringAbstract Background Wheat seeds display different colors due to the types and contents of anthocyanins, which is closely related to anthocyanin metabolism. In this study, a transcriptomic and metabolomic analysis between white and purple color wheat pericarp aimed to explore some key genes and metabolites involved in anthocyanin metabolism. Results Two wheat cultivars, a white seed cultivar Shiluan02-1 and purple seed cultivar Hengzi151 were used to identify the variations in differentially expressed genes (DEGs) and differentially accumulated flavonoids (DAFs). Based on metabolomic data, 314 metabolites and 191 DAFs were identified. Chalcone, flavonol, pro-anthocyanidin and anthocyanidin were the most differentially accumulated flavonoid compounds in Hengzi151. 2610 up-regulated and 2668 down-regulated DEGs were identified according to transcriptomic data. The results showed that some structural genes in anthocyanin synthesis pathway were prominently activated in Hengzi151, such as PAL, CAD, CHS and so on. Transcription factors (TFs) of MYB, bHLH, WD40 and some other TFs probably involved in regulating anthocyanin biosynthesis were identified. Some genes from hormone synthetic and signaling pathways that may participate in regulating anthocyanin biosynthesis also have been identified. Conclusions Our results provide valuable information on the candidate genes and metabolites involved in the anthocyanin metabolism in wheat pericarp.https://doi.org/10.1186/s12863-024-01294-yWheat pericarpAnthocyaninMetabolomicTranscriptomicHormone |
| spellingShingle | Jiao Wang Lei Sun Bo Jiao Pu Zhao Tianyun Xu Sa Gu Chenmin Huo Jianzhou Pang Shuo Zhou Integrated metabolomic and transcriptomic analysis of anthocyanin metabolism in wheat pericarp BMC Genomic Data Wheat pericarp Anthocyanin Metabolomic Transcriptomic Hormone |
| title | Integrated metabolomic and transcriptomic analysis of anthocyanin metabolism in wheat pericarp |
| title_full | Integrated metabolomic and transcriptomic analysis of anthocyanin metabolism in wheat pericarp |
| title_fullStr | Integrated metabolomic and transcriptomic analysis of anthocyanin metabolism in wheat pericarp |
| title_full_unstemmed | Integrated metabolomic and transcriptomic analysis of anthocyanin metabolism in wheat pericarp |
| title_short | Integrated metabolomic and transcriptomic analysis of anthocyanin metabolism in wheat pericarp |
| title_sort | integrated metabolomic and transcriptomic analysis of anthocyanin metabolism in wheat pericarp |
| topic | Wheat pericarp Anthocyanin Metabolomic Transcriptomic Hormone |
| url | https://doi.org/10.1186/s12863-024-01294-y |
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