NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidia <i>Vairimorpha (Nosema) ceranae</i>
<i>Vairimorpha (Nosema) ceranae</i> is a pathogen that affects <i>Apis mellifera</i> and <i>Apis ceranae</i> Fabricius, capable of spreading within and between honeybee colonies. The spore wall of microsporidia is the initial structure to contact the host cell dir...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/1/142 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587894312665088 |
|---|---|
| author | Pengfei Wang Dufu Li Qianmin Hai Siming Liu Yueyue Zhang Jun Zhang Jinshan Xu Zhengang Ma Zeyang Zhou |
| author_facet | Pengfei Wang Dufu Li Qianmin Hai Siming Liu Yueyue Zhang Jun Zhang Jinshan Xu Zhengang Ma Zeyang Zhou |
| author_sort | Pengfei Wang |
| collection | DOAJ |
| description | <i>Vairimorpha (Nosema) ceranae</i> is a pathogen that affects <i>Apis mellifera</i> and <i>Apis ceranae</i> Fabricius, capable of spreading within and between honeybee colonies. The spore wall of microsporidia is the initial structure to contact the host cell directly, which may play a crucial role in the infection process. Currently, several spore wall proteins have been identified in microsporidia, but only two spore wall proteins from <i>V. ceranae</i> have been characterized. Here, we report the expression and identification of a novel spore wall protein, NcSWP8, with a molecular mass of 21.37 kDa in <i>V. ceranae</i>. Subcellular localization analysis revealed that NcSWP8 was localized on the spore wall of <i>V. ceranae</i>. Co-immunoprecipitation and Far-Western blotting experiments demonstrated that NcSWP8 could stably interact with polar tube proteins, NcPTP2 and NcPTP3. The antibody blocking assay significantly decreased their infection rate, indicating that NcSWP8 played a significant role in the process of <i>V. ceranae</i> infection. These results together suggested that NcSWP8 was a new spore wall protein localized to the spore wall and interacted with the polar tube proteins, playing a crucial role in supporting the formation of the spore wall and potentially affecting the process of infection of <i>V. ceranae</i>. |
| format | Article |
| id | doaj-art-f634c0aea7d1441b828bb5c86b7f6c7d |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-f634c0aea7d1441b828bb5c86b7f6c7d2025-01-24T13:42:48ZengMDPI AGMicroorganisms2076-26072025-01-0113114210.3390/microorganisms13010142NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidia <i>Vairimorpha (Nosema) ceranae</i>Pengfei Wang0Dufu Li1Qianmin Hai2Siming Liu3Yueyue Zhang4Jun Zhang5Jinshan Xu6Zhengang Ma7Zeyang Zhou8Key Laboratory of Pollinator Resources Conservation and Utilization of the Upper Yangtze River, Ministry of Agriculture and Rural Affairs, Chongqing Normal University, Chongqing 401331, ChinaKey Laboratory of Pollinator Resources Conservation and Utilization of the Upper Yangtze River, Ministry of Agriculture and Rural Affairs, Chongqing Normal University, Chongqing 401331, ChinaKey Laboratory of Pollinator Resources Conservation and Utilization of the Upper Yangtze River, Ministry of Agriculture and Rural Affairs, Chongqing Normal University, Chongqing 401331, ChinaKey Laboratory of Pollinator Resources Conservation and Utilization of the Upper Yangtze River, Ministry of Agriculture and Rural Affairs, Chongqing Normal University, Chongqing 401331, ChinaKey Laboratory of Pollinator Resources Conservation and Utilization of the Upper Yangtze River, Ministry of Agriculture and Rural Affairs, Chongqing Normal University, Chongqing 401331, ChinaKey Laboratory of Pollinator Resources Conservation and Utilization of the Upper Yangtze River, Ministry of Agriculture and Rural Affairs, Chongqing Normal University, Chongqing 401331, ChinaKey Laboratory of Pollinator Resources Conservation and Utilization of the Upper Yangtze River, Ministry of Agriculture and Rural Affairs, Chongqing Normal University, Chongqing 401331, ChinaKey Laboratory of Pollinator Resources Conservation and Utilization of the Upper Yangtze River, Ministry of Agriculture and Rural Affairs, Chongqing Normal University, Chongqing 401331, ChinaKey Laboratory of Pollinator Resources Conservation and Utilization of the Upper Yangtze River, Ministry of Agriculture and Rural Affairs, Chongqing Normal University, Chongqing 401331, China<i>Vairimorpha (Nosema) ceranae</i> is a pathogen that affects <i>Apis mellifera</i> and <i>Apis ceranae</i> Fabricius, capable of spreading within and between honeybee colonies. The spore wall of microsporidia is the initial structure to contact the host cell directly, which may play a crucial role in the infection process. Currently, several spore wall proteins have been identified in microsporidia, but only two spore wall proteins from <i>V. ceranae</i> have been characterized. Here, we report the expression and identification of a novel spore wall protein, NcSWP8, with a molecular mass of 21.37 kDa in <i>V. ceranae</i>. Subcellular localization analysis revealed that NcSWP8 was localized on the spore wall of <i>V. ceranae</i>. Co-immunoprecipitation and Far-Western blotting experiments demonstrated that NcSWP8 could stably interact with polar tube proteins, NcPTP2 and NcPTP3. The antibody blocking assay significantly decreased their infection rate, indicating that NcSWP8 played a significant role in the process of <i>V. ceranae</i> infection. These results together suggested that NcSWP8 was a new spore wall protein localized to the spore wall and interacted with the polar tube proteins, playing a crucial role in supporting the formation of the spore wall and potentially affecting the process of infection of <i>V. ceranae</i>.https://www.mdpi.com/2076-2607/13/1/142<i>Vairimorpha (Nosema) ceranae</i>spore wall proteinpolar tube proteinssubcellular localizationbiological functions |
| spellingShingle | Pengfei Wang Dufu Li Qianmin Hai Siming Liu Yueyue Zhang Jun Zhang Jinshan Xu Zhengang Ma Zeyang Zhou NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidia <i>Vairimorpha (Nosema) ceranae</i> Microorganisms <i>Vairimorpha (Nosema) ceranae</i> spore wall protein polar tube proteins subcellular localization biological functions |
| title | NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidia <i>Vairimorpha (Nosema) ceranae</i> |
| title_full | NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidia <i>Vairimorpha (Nosema) ceranae</i> |
| title_fullStr | NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidia <i>Vairimorpha (Nosema) ceranae</i> |
| title_full_unstemmed | NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidia <i>Vairimorpha (Nosema) ceranae</i> |
| title_short | NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic Microsporidia <i>Vairimorpha (Nosema) ceranae</i> |
| title_sort | ncswp8 a new spore wall protein interacts with polar tube proteins in the parasitic microsporidia i vairimorpha nosema ceranae i |
| topic | <i>Vairimorpha (Nosema) ceranae</i> spore wall protein polar tube proteins subcellular localization biological functions |
| url | https://www.mdpi.com/2076-2607/13/1/142 |
| work_keys_str_mv | AT pengfeiwang ncswp8anewsporewallproteininteractswithpolartubeproteinsintheparasiticmicrosporidiaivairimorphanosemaceranaei AT dufuli ncswp8anewsporewallproteininteractswithpolartubeproteinsintheparasiticmicrosporidiaivairimorphanosemaceranaei AT qianminhai ncswp8anewsporewallproteininteractswithpolartubeproteinsintheparasiticmicrosporidiaivairimorphanosemaceranaei AT simingliu ncswp8anewsporewallproteininteractswithpolartubeproteinsintheparasiticmicrosporidiaivairimorphanosemaceranaei AT yueyuezhang ncswp8anewsporewallproteininteractswithpolartubeproteinsintheparasiticmicrosporidiaivairimorphanosemaceranaei AT junzhang ncswp8anewsporewallproteininteractswithpolartubeproteinsintheparasiticmicrosporidiaivairimorphanosemaceranaei AT jinshanxu ncswp8anewsporewallproteininteractswithpolartubeproteinsintheparasiticmicrosporidiaivairimorphanosemaceranaei AT zhengangma ncswp8anewsporewallproteininteractswithpolartubeproteinsintheparasiticmicrosporidiaivairimorphanosemaceranaei AT zeyangzhou ncswp8anewsporewallproteininteractswithpolartubeproteinsintheparasiticmicrosporidiaivairimorphanosemaceranaei |