LncMPEG1/miR-2c regulates exoskeleton biocalcification via engrailed in bivalve
LncRNAs and miRNAs has been reported to play important regulation roles in skeleton growth. This research elucidates the molecular interplay between long noncoding RNA LncMPEG1 and miRNA miR-2c in the biocalcification process of shell formation. The study establishes LncMPEG1 as a molecular sponge f...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342500002X |
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| author | Minxin Liang Caixia Cai Ziqi Ren Zhiqing Li Ronglian Huang Qingheng Wang Yongshan Liao Chuangye Yang Zhe Zheng |
| author_facet | Minxin Liang Caixia Cai Ziqi Ren Zhiqing Li Ronglian Huang Qingheng Wang Yongshan Liao Chuangye Yang Zhe Zheng |
| author_sort | Minxin Liang |
| collection | DOAJ |
| description | LncRNAs and miRNAs has been reported to play important regulation roles in skeleton growth. This research elucidates the molecular interplay between long noncoding RNA LncMPEG1 and miRNA miR-2c in the biocalcification process of shell formation. The study establishes LncMPEG1 as a molecular sponge for miR-2c, modulating the expression of key biomineralization genes, including homeobox protein engrailed and matrix metalloproteases (MMPs). Utilizing miRanda prediction, interaction sites between miR-2c and LncMPEG1 were identified and validated through RNA immunoprecipitation and luciferase assays. In vivo overexpression of miR-2c suppressed engrailed and MMP expression, while miR-2c inhibition induced the opposite effect. Luciferase assays confirmed miR-2c's inhibitory effect on the 3′UTR of target genes (p < 0.001). Western blot analysis corroborated these findings, showing miR-2c's regulatory impact on protein expression levels. LncMPEG1 interference significantly reduced target gene transcription (p < 0.01), and its overexpression enhanced the reporter vector's activity by binding miR-2c (p < 0.05). Collectively, these results underscore LncMPEG1's role in shell formation through competitive binding with miR-2c, thereby regulating the expression of biomineralization-related genes. |
| format | Article |
| id | doaj-art-f5fddc4a1daf434d94fe0794664b3561 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-f5fddc4a1daf434d94fe0794664b35612025-02-06T05:12:19ZengElsevierAquaculture Reports2352-51342025-03-0140102616LncMPEG1/miR-2c regulates exoskeleton biocalcification via engrailed in bivalveMinxin Liang0Caixia Cai1Ziqi Ren2Zhiqing Li3Ronglian Huang4Qingheng Wang5Yongshan Liao6Chuangye Yang7Zhe Zheng8Fisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Development and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Development and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, China; Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, China; Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, ChinaFisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Development and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Corresponding author at: Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.LncRNAs and miRNAs has been reported to play important regulation roles in skeleton growth. This research elucidates the molecular interplay between long noncoding RNA LncMPEG1 and miRNA miR-2c in the biocalcification process of shell formation. The study establishes LncMPEG1 as a molecular sponge for miR-2c, modulating the expression of key biomineralization genes, including homeobox protein engrailed and matrix metalloproteases (MMPs). Utilizing miRanda prediction, interaction sites between miR-2c and LncMPEG1 were identified and validated through RNA immunoprecipitation and luciferase assays. In vivo overexpression of miR-2c suppressed engrailed and MMP expression, while miR-2c inhibition induced the opposite effect. Luciferase assays confirmed miR-2c's inhibitory effect on the 3′UTR of target genes (p < 0.001). Western blot analysis corroborated these findings, showing miR-2c's regulatory impact on protein expression levels. LncMPEG1 interference significantly reduced target gene transcription (p < 0.01), and its overexpression enhanced the reporter vector's activity by binding miR-2c (p < 0.05). Collectively, these results underscore LncMPEG1's role in shell formation through competitive binding with miR-2c, thereby regulating the expression of biomineralization-related genes.http://www.sciencedirect.com/science/article/pii/S235251342500002XPinctada fucata martensiiShell growthLncMPEG1miR-2c |
| spellingShingle | Minxin Liang Caixia Cai Ziqi Ren Zhiqing Li Ronglian Huang Qingheng Wang Yongshan Liao Chuangye Yang Zhe Zheng LncMPEG1/miR-2c regulates exoskeleton biocalcification via engrailed in bivalve Aquaculture Reports Pinctada fucata martensii Shell growth LncMPEG1 miR-2c |
| title | LncMPEG1/miR-2c regulates exoskeleton biocalcification via engrailed in bivalve |
| title_full | LncMPEG1/miR-2c regulates exoskeleton biocalcification via engrailed in bivalve |
| title_fullStr | LncMPEG1/miR-2c regulates exoskeleton biocalcification via engrailed in bivalve |
| title_full_unstemmed | LncMPEG1/miR-2c regulates exoskeleton biocalcification via engrailed in bivalve |
| title_short | LncMPEG1/miR-2c regulates exoskeleton biocalcification via engrailed in bivalve |
| title_sort | lncmpeg1 mir 2c regulates exoskeleton biocalcification via engrailed in bivalve |
| topic | Pinctada fucata martensii Shell growth LncMPEG1 miR-2c |
| url | http://www.sciencedirect.com/science/article/pii/S235251342500002X |
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