The <i>Pyrus sinkiangensis Yu PsLEA4</i> Gene Enhances the Cold Resistance of <i>Solanum lycopersicum</i>
Plants have large amounts of the late embryogenesis abundant protein (LEA) family of proteins, which is involved in osmotic regulation. The Korla Pear (<i>Pyrus sinkiangensis Yu</i>) is an uncommon pear species that thrives in Xinjiang and can survive below-freezing conditions. We found...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/2/180 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587683567763456 |
|---|---|
| author | Xueying Yang Wenjuan Zhao Hui Li Zhenxia Zhao Jianbo Zhu Jin Li |
| author_facet | Xueying Yang Wenjuan Zhao Hui Li Zhenxia Zhao Jianbo Zhu Jin Li |
| author_sort | Xueying Yang |
| collection | DOAJ |
| description | Plants have large amounts of the late embryogenesis abundant protein (LEA) family of proteins, which is involved in osmotic regulation. The Korla Pear (<i>Pyrus sinkiangensis Yu</i>) is an uncommon pear species that thrives in Xinjiang and can survive below-freezing conditions. We found that the <i>PsLEA4</i> gene was more expressed after cold treatment by looking at the transcriptome data of the Korla Pear. In order to evaluate the biological function of the <i>PsLEA4</i> protein under low-temperature stress and its potential for use in agricultural breeding, we cloned the <i>PsLEA4</i> gene from the Korla Pear, made a plant overexpression vector, and transformed it into a tomato via Agrobacterium transformation. When exposed to low temperatures, we found that <i>PsLEA4</i> overexpression can regulate proline metabolism and antioxidant enzyme activity in tomatoes compared to wild tomatoes. Because of this, transgenic tomatoes are more resilient to cold temperatures and produce more than their wild counterparts. Thus, expressing <i>PsLEA4</i> has multiple advantages: (1) Improving frost resistance and reducing plant damage. (2) Increasing crop yield. Therefore, this study provides a theoretical basis for the role of the <i>PsLEA4</i> protein in plants’ resilience to low temperatures, as well as for its potential application in crop breeding. |
| format | Article |
| id | doaj-art-f5ee163044a34e97a55ca20972dc8763 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-f5ee163044a34e97a55ca20972dc87632025-01-24T13:46:38ZengMDPI AGPlants2223-77472025-01-0114218010.3390/plants14020180The <i>Pyrus sinkiangensis Yu PsLEA4</i> Gene Enhances the Cold Resistance of <i>Solanum lycopersicum</i>Xueying Yang0Wenjuan Zhao1Hui Li2Zhenxia Zhao3Jianbo Zhu4Jin Li5College of Life Sciences, Shihezi University, Shihezi 832000, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832000, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832000, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832000, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832000, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832000, ChinaPlants have large amounts of the late embryogenesis abundant protein (LEA) family of proteins, which is involved in osmotic regulation. The Korla Pear (<i>Pyrus sinkiangensis Yu</i>) is an uncommon pear species that thrives in Xinjiang and can survive below-freezing conditions. We found that the <i>PsLEA4</i> gene was more expressed after cold treatment by looking at the transcriptome data of the Korla Pear. In order to evaluate the biological function of the <i>PsLEA4</i> protein under low-temperature stress and its potential for use in agricultural breeding, we cloned the <i>PsLEA4</i> gene from the Korla Pear, made a plant overexpression vector, and transformed it into a tomato via Agrobacterium transformation. When exposed to low temperatures, we found that <i>PsLEA4</i> overexpression can regulate proline metabolism and antioxidant enzyme activity in tomatoes compared to wild tomatoes. Because of this, transgenic tomatoes are more resilient to cold temperatures and produce more than their wild counterparts. Thus, expressing <i>PsLEA4</i> has multiple advantages: (1) Improving frost resistance and reducing plant damage. (2) Increasing crop yield. Therefore, this study provides a theoretical basis for the role of the <i>PsLEA4</i> protein in plants’ resilience to low temperatures, as well as for its potential application in crop breeding.https://www.mdpi.com/2223-7747/14/2/180LEA<i>Pyrus sinkiangensis Yu</i>low temperatureabiotic stresstransgenic tomato |
| spellingShingle | Xueying Yang Wenjuan Zhao Hui Li Zhenxia Zhao Jianbo Zhu Jin Li The <i>Pyrus sinkiangensis Yu PsLEA4</i> Gene Enhances the Cold Resistance of <i>Solanum lycopersicum</i> Plants LEA <i>Pyrus sinkiangensis Yu</i> low temperature abiotic stress transgenic tomato |
| title | The <i>Pyrus sinkiangensis Yu PsLEA4</i> Gene Enhances the Cold Resistance of <i>Solanum lycopersicum</i> |
| title_full | The <i>Pyrus sinkiangensis Yu PsLEA4</i> Gene Enhances the Cold Resistance of <i>Solanum lycopersicum</i> |
| title_fullStr | The <i>Pyrus sinkiangensis Yu PsLEA4</i> Gene Enhances the Cold Resistance of <i>Solanum lycopersicum</i> |
| title_full_unstemmed | The <i>Pyrus sinkiangensis Yu PsLEA4</i> Gene Enhances the Cold Resistance of <i>Solanum lycopersicum</i> |
| title_short | The <i>Pyrus sinkiangensis Yu PsLEA4</i> Gene Enhances the Cold Resistance of <i>Solanum lycopersicum</i> |
| title_sort | i pyrus sinkiangensis yu pslea4 i gene enhances the cold resistance of i solanum lycopersicum i |
| topic | LEA <i>Pyrus sinkiangensis Yu</i> low temperature abiotic stress transgenic tomato |
| url | https://www.mdpi.com/2223-7747/14/2/180 |
| work_keys_str_mv | AT xueyingyang theipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi AT wenjuanzhao theipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi AT huili theipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi AT zhenxiazhao theipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi AT jianbozhu theipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi AT jinli theipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi AT xueyingyang ipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi AT wenjuanzhao ipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi AT huili ipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi AT zhenxiazhao ipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi AT jianbozhu ipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi AT jinli ipyrussinkiangensisyupslea4igeneenhancesthecoldresistanceofisolanumlycopersicumi |