Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo
MicroRNAs (miRs) are involved in key biological processes via suppression of gene expression at posttranscriptional levels. According to their superior functions, subtle modulation of miR expression by certain compounds or nutrients is desirable under particular conditions. Bacterial lipopolysacchar...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2009/415437 |
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| _version_ | 1832558631127613440 |
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| author | Constance Schmelzer Mitsuaki Kitano Gerald Rimbach Petra Niklowitz Thomas Menke Kazunori Hosoe Frank Döring |
| author_facet | Constance Schmelzer Mitsuaki Kitano Gerald Rimbach Petra Niklowitz Thomas Menke Kazunori Hosoe Frank Döring |
| author_sort | Constance Schmelzer |
| collection | DOAJ |
| description | MicroRNAs (miRs) are involved in key biological processes via suppression of gene expression at posttranscriptional levels. According to their superior functions, subtle modulation of miR expression by certain compounds or nutrients is desirable under particular conditions. Bacterial lipopolysaccharide (LPS) induces a reactive oxygen species-/NF-κB-dependent pathway which increases the expression of the anti-inflammatory miR-146a. We hypothesized that this induction could be modulated by the antioxidant ubiquinol-10. Preincubation of human monocytic THP-1 cells with ubiquinol-10 reduced the LPS-induced expression level of miR-146a to 78.9 ± 13.22%. In liver samples of mice injected with LPS, supplementation with ubiquinol-10 leads to a reduction of LPS-induced miR-146a expression to 78.12 ± 21.25%. From these consistent in vitro and in vivo data, we conclude that ubiquinol-10 may fine-tune the inflammatory response via moderate reduction of miR-146a expression. |
| format | Article |
| id | doaj-art-f5e65642ae4c439ea60d81c425e81ed2 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-f5e65642ae4c439ea60d81c425e81ed22025-02-03T01:32:01ZengWileyMediators of Inflammation0962-93511466-18612009-01-01200910.1155/2009/415437415437Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In VivoConstance Schmelzer0Mitsuaki Kitano1Gerald Rimbach2Petra Niklowitz3Thomas Menke4Kazunori Hosoe5Frank Döring6Institute of Human Nutrition and Food Science, Molecular Nutrition, Christian-Albrechts-University of Kiel, Heinrich-Hecht-Platz 10, 24118 Kiel, GermanyFrontier Biochemical and Medical Research Laboratories, Kaneka Corporation, Takasago, Hyogo, JapanInstitute of Human Nutrition and Food Science, Food Science, Christian-Albrechts-University of Kiel, Hermann-Rodewald-Street 6, 24098 Kiel, GermanyVestische Kinder-und Jugendklinik Datteln, Universität Written/Herdecke, Dr.-Friedrich-Steiner Street 5, 45711 Datteln, GermanyVestische Kinder-und Jugendklinik Datteln, Universität Written/Herdecke, Dr.-Friedrich-Steiner Street 5, 45711 Datteln, GermanyFunctional Food Ingredients Division, Kaneka Corporation, Osaka, JapanInstitute of Human Nutrition and Food Science, Molecular Nutrition, Christian-Albrechts-University of Kiel, Heinrich-Hecht-Platz 10, 24118 Kiel, GermanyMicroRNAs (miRs) are involved in key biological processes via suppression of gene expression at posttranscriptional levels. According to their superior functions, subtle modulation of miR expression by certain compounds or nutrients is desirable under particular conditions. Bacterial lipopolysaccharide (LPS) induces a reactive oxygen species-/NF-κB-dependent pathway which increases the expression of the anti-inflammatory miR-146a. We hypothesized that this induction could be modulated by the antioxidant ubiquinol-10. Preincubation of human monocytic THP-1 cells with ubiquinol-10 reduced the LPS-induced expression level of miR-146a to 78.9 ± 13.22%. In liver samples of mice injected with LPS, supplementation with ubiquinol-10 leads to a reduction of LPS-induced miR-146a expression to 78.12 ± 21.25%. From these consistent in vitro and in vivo data, we conclude that ubiquinol-10 may fine-tune the inflammatory response via moderate reduction of miR-146a expression.http://dx.doi.org/10.1155/2009/415437 |
| spellingShingle | Constance Schmelzer Mitsuaki Kitano Gerald Rimbach Petra Niklowitz Thomas Menke Kazunori Hosoe Frank Döring Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo Mediators of Inflammation |
| title | Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo |
| title_full | Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo |
| title_fullStr | Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo |
| title_full_unstemmed | Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo |
| title_short | Effects of Ubiquinol-10 on MicroRNA-146a Expression In Vitro and In Vivo |
| title_sort | effects of ubiquinol 10 on microrna 146a expression in vitro and in vivo |
| url | http://dx.doi.org/10.1155/2009/415437 |
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