Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage
Background. Neural crest-derived mesenchymal stem cells (MSCs) from human oral tissues possess immunomodulatory and regenerative properties and are emerging as a potential therapeutic tool to treat diverse diseases, such as multiple sclerosis, myocardial infarction, and connective tissue damages. In...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/5651287 |
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| author | Francesca Diomede Thangavelu Soundara Rajan Valentina Gatta Marco D’Aurora Ilaria Merciaro Marco Marchisio Aurelio Muttini Sergio Caputi Placido Bramanti Emanuela Mazzon Oriana Trubiani |
| author_facet | Francesca Diomede Thangavelu Soundara Rajan Valentina Gatta Marco D’Aurora Ilaria Merciaro Marco Marchisio Aurelio Muttini Sergio Caputi Placido Bramanti Emanuela Mazzon Oriana Trubiani |
| author_sort | Francesca Diomede |
| collection | DOAJ |
| description | Background. Neural crest-derived mesenchymal stem cells (MSCs) from human oral tissues possess immunomodulatory and regenerative properties and are emerging as a potential therapeutic tool to treat diverse diseases, such as multiple sclerosis, myocardial infarction, and connective tissue damages. In addition to cell-surface antigens, dental MSCs express embryonic stem cell markers as neural crest cells originate from the ectoderm layer. In vitro passages may eventually modify these embryonic marker expressions and other stemness properties, including proliferation. In the present study, we have investigated the expression of proteins involved in cell proliferation/senescence and embryonic stem cell markers during early (passage 2) and late passages (passage 15) in MSCs obtained from human gingiva, periodontal, and dental pulp tissues. Methods. Cell proliferation assay, beta galactosidase staining, immunocytochemistry, and real-time PCR techniques were applied. Results. Cell proliferation assay showed no difference between early and late passages while senescence markers p16 and p21 were considerably increased in late passage. Embryonic stem cell markers including SKIL, MEIS1, and JARID2 were differentially modulated between P2 and P15 cells. Discussion. Our results suggest that the presence of embryonic and proliferation markers even in late passage may potentially endorse the application of dental-derived MSCs in stem cell therapy-based clinical trials. |
| format | Article |
| id | doaj-art-f5e18719daa64506948e5dd0ad5fe52d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-f5e18719daa64506948e5dd0ad5fe52d2025-02-03T01:27:11ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/56512875651287Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term PassageFrancesca Diomede0Thangavelu Soundara Rajan1Valentina Gatta2Marco D’Aurora3Ilaria Merciaro4Marco Marchisio5Aurelio Muttini6Sergio Caputi7Placido Bramanti8Emanuela Mazzon9Oriana Trubiani10Stem Cells and Regenerative Medicine Laboratory, Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyDepartment of Psychological, Health and Territorial Sciences, School of Medicine, University “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Psychological, Health and Territorial Sciences, School of Medicine, University “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyStem Cells and Regenerative Medicine Laboratory, Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Medicine and Aging Science, University “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyDepartment of Comparative Biomedical Sciences, University of Teramo, Via Balzarini 1, 64100 Teramo, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyStem Cells and Regenerative Medicine Laboratory, Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, ItalyBackground. Neural crest-derived mesenchymal stem cells (MSCs) from human oral tissues possess immunomodulatory and regenerative properties and are emerging as a potential therapeutic tool to treat diverse diseases, such as multiple sclerosis, myocardial infarction, and connective tissue damages. In addition to cell-surface antigens, dental MSCs express embryonic stem cell markers as neural crest cells originate from the ectoderm layer. In vitro passages may eventually modify these embryonic marker expressions and other stemness properties, including proliferation. In the present study, we have investigated the expression of proteins involved in cell proliferation/senescence and embryonic stem cell markers during early (passage 2) and late passages (passage 15) in MSCs obtained from human gingiva, periodontal, and dental pulp tissues. Methods. Cell proliferation assay, beta galactosidase staining, immunocytochemistry, and real-time PCR techniques were applied. Results. Cell proliferation assay showed no difference between early and late passages while senescence markers p16 and p21 were considerably increased in late passage. Embryonic stem cell markers including SKIL, MEIS1, and JARID2 were differentially modulated between P2 and P15 cells. Discussion. Our results suggest that the presence of embryonic and proliferation markers even in late passage may potentially endorse the application of dental-derived MSCs in stem cell therapy-based clinical trials.http://dx.doi.org/10.1155/2017/5651287 |
| spellingShingle | Francesca Diomede Thangavelu Soundara Rajan Valentina Gatta Marco D’Aurora Ilaria Merciaro Marco Marchisio Aurelio Muttini Sergio Caputi Placido Bramanti Emanuela Mazzon Oriana Trubiani Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage Stem Cells International |
| title | Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage |
| title_full | Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage |
| title_fullStr | Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage |
| title_full_unstemmed | Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage |
| title_short | Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage |
| title_sort | stemness maintenance properties in human oral stem cells after long term passage |
| url | http://dx.doi.org/10.1155/2017/5651287 |
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