Isolation and characterization of a novel highly efficient bacterium Lysinibacillus boronitolerans QD4 for quantum dot biosynthesis
Microorganism-based biosynthesis of quantum dots is a low-cost and green production method with a wide range of potential applications. The development of environmentally friendly synthesis methods is required due to the toxicity and severe reactions that occur during the chemical synthesis of quant...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1521632/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832582921370730496 |
|---|---|
| author | Xingyu Gu Xingyu Gu Xiaoju Li Ruijia Zhang Ruijia Zhang Ruoli Zheng Ruoli Zheng Mingrui Li Mingrui Li Rong Huang Xin Pang |
| author_facet | Xingyu Gu Xingyu Gu Xiaoju Li Ruijia Zhang Ruijia Zhang Ruoli Zheng Ruoli Zheng Mingrui Li Mingrui Li Rong Huang Xin Pang |
| author_sort | Xingyu Gu |
| collection | DOAJ |
| description | Microorganism-based biosynthesis of quantum dots is a low-cost and green production method with a wide range of potential applications. The development of environmentally friendly synthesis methods is required due to the toxicity and severe reactions that occur during the chemical synthesis of quantum dots. In this study, a novel strain, QD4, with the ability to the effectively and rapidly biosynthesize CdS quantum dots, is isolated and reported. The isolated strain is a Gram-positive, aerobic, flagellated, and rod-shaped bacterium, isolated from seawater. Through the physio-biochemical characterization and 16S rRNA-based phylogenetic tree analysis, the strain is identified as Lysinibacillus boronitolerans QD4. The strain QD4 grows well in the range of 25–40 °C (optimum, 37 °C), pH 5.0–9.0 (optimum, pH 7.0), with a high cadmium-resistance as it could grow at Cd2+ concentration up to 2 mM, implying its good adaptability to the environment and potential for application. Cd2+ and L-cysteine are used as substrates for the biosynthesis of CdS quantum dots by strain QD4. The distinctive yellow fluorescence from CdS quantum dots is visible after only a short induction time (a few hours). Moreover, the properties of the CdS quantum dots are characterized by fluorescence spectroscopy, UV-absorption spectroscopy, TEM, XRD, XPS, and infrared spectroscopy. This study provides a novel strain resource for efficient biosynthesis of extracellular, water-soluble quantum dots, paving potential industrial applications in green production. |
| format | Article |
| id | doaj-art-f589cd91cc4d45bda9581ac8f09dc412 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-f589cd91cc4d45bda9581ac8f09dc4122025-01-29T06:46:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.15216321521632Isolation and characterization of a novel highly efficient bacterium Lysinibacillus boronitolerans QD4 for quantum dot biosynthesisXingyu Gu0Xingyu Gu1Xiaoju Li2Ruijia Zhang3Ruijia Zhang4Ruoli Zheng5Ruoli Zheng6Mingrui Li7Mingrui Li8Rong Huang9Xin Pang10School of Life Sciences, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaSchool of Life Sciences, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaSchool of Life Sciences, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaSchool of Life Sciences, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaMicroorganism-based biosynthesis of quantum dots is a low-cost and green production method with a wide range of potential applications. The development of environmentally friendly synthesis methods is required due to the toxicity and severe reactions that occur during the chemical synthesis of quantum dots. In this study, a novel strain, QD4, with the ability to the effectively and rapidly biosynthesize CdS quantum dots, is isolated and reported. The isolated strain is a Gram-positive, aerobic, flagellated, and rod-shaped bacterium, isolated from seawater. Through the physio-biochemical characterization and 16S rRNA-based phylogenetic tree analysis, the strain is identified as Lysinibacillus boronitolerans QD4. The strain QD4 grows well in the range of 25–40 °C (optimum, 37 °C), pH 5.0–9.0 (optimum, pH 7.0), with a high cadmium-resistance as it could grow at Cd2+ concentration up to 2 mM, implying its good adaptability to the environment and potential for application. Cd2+ and L-cysteine are used as substrates for the biosynthesis of CdS quantum dots by strain QD4. The distinctive yellow fluorescence from CdS quantum dots is visible after only a short induction time (a few hours). Moreover, the properties of the CdS quantum dots are characterized by fluorescence spectroscopy, UV-absorption spectroscopy, TEM, XRD, XPS, and infrared spectroscopy. This study provides a novel strain resource for efficient biosynthesis of extracellular, water-soluble quantum dots, paving potential industrial applications in green production.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1521632/fullLysinibacillus boronitoleransquantum dotnanoparticle biosynthesiscadmium sulfideextracellular |
| spellingShingle | Xingyu Gu Xingyu Gu Xiaoju Li Ruijia Zhang Ruijia Zhang Ruoli Zheng Ruoli Zheng Mingrui Li Mingrui Li Rong Huang Xin Pang Isolation and characterization of a novel highly efficient bacterium Lysinibacillus boronitolerans QD4 for quantum dot biosynthesis Frontiers in Microbiology Lysinibacillus boronitolerans quantum dot nanoparticle biosynthesis cadmium sulfide extracellular |
| title | Isolation and characterization of a novel highly efficient bacterium Lysinibacillus boronitolerans QD4 for quantum dot biosynthesis |
| title_full | Isolation and characterization of a novel highly efficient bacterium Lysinibacillus boronitolerans QD4 for quantum dot biosynthesis |
| title_fullStr | Isolation and characterization of a novel highly efficient bacterium Lysinibacillus boronitolerans QD4 for quantum dot biosynthesis |
| title_full_unstemmed | Isolation and characterization of a novel highly efficient bacterium Lysinibacillus boronitolerans QD4 for quantum dot biosynthesis |
| title_short | Isolation and characterization of a novel highly efficient bacterium Lysinibacillus boronitolerans QD4 for quantum dot biosynthesis |
| title_sort | isolation and characterization of a novel highly efficient bacterium lysinibacillus boronitolerans qd4 for quantum dot biosynthesis |
| topic | Lysinibacillus boronitolerans quantum dot nanoparticle biosynthesis cadmium sulfide extracellular |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1521632/full |
| work_keys_str_mv | AT xingyugu isolationandcharacterizationofanovelhighlyefficientbacteriumlysinibacillusboronitoleransqd4forquantumdotbiosynthesis AT xingyugu isolationandcharacterizationofanovelhighlyefficientbacteriumlysinibacillusboronitoleransqd4forquantumdotbiosynthesis AT xiaojuli isolationandcharacterizationofanovelhighlyefficientbacteriumlysinibacillusboronitoleransqd4forquantumdotbiosynthesis AT ruijiazhang isolationandcharacterizationofanovelhighlyefficientbacteriumlysinibacillusboronitoleransqd4forquantumdotbiosynthesis AT ruijiazhang isolationandcharacterizationofanovelhighlyefficientbacteriumlysinibacillusboronitoleransqd4forquantumdotbiosynthesis AT ruolizheng isolationandcharacterizationofanovelhighlyefficientbacteriumlysinibacillusboronitoleransqd4forquantumdotbiosynthesis AT ruolizheng isolationandcharacterizationofanovelhighlyefficientbacteriumlysinibacillusboronitoleransqd4forquantumdotbiosynthesis AT mingruili isolationandcharacterizationofanovelhighlyefficientbacteriumlysinibacillusboronitoleransqd4forquantumdotbiosynthesis AT mingruili isolationandcharacterizationofanovelhighlyefficientbacteriumlysinibacillusboronitoleransqd4forquantumdotbiosynthesis AT ronghuang isolationandcharacterizationofanovelhighlyefficientbacteriumlysinibacillusboronitoleransqd4forquantumdotbiosynthesis AT xinpang isolationandcharacterizationofanovelhighlyefficientbacteriumlysinibacillusboronitoleransqd4forquantumdotbiosynthesis |