Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves
We investigated the antiplasmodial properties of crude extracts from Carica papaya leaves to trace the activity through bioassay-guided fractionation. The greatest antiplasmodial activity was observed in the ethyl acetate crude extract. C. papaya showed a high selectivity for P. falciparum against C...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2011/104954 |
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| author | Paula Melariri William Campbell Paschal Etusim Peter Smith |
| author_facet | Paula Melariri William Campbell Paschal Etusim Peter Smith |
| author_sort | Paula Melariri |
| collection | DOAJ |
| description | We investigated the antiplasmodial properties of crude extracts from Carica papaya leaves to trace the activity through bioassay-guided fractionation. The greatest antiplasmodial activity was observed in the ethyl acetate crude extract. C. papaya showed a high selectivity for P. falciparum against CHO cells with a selectivity index of 249.25 and 185.37 in the chloroquine-sensitive D10 and chloroquine-resistant DD2 strains, respectively. Carica papaya ethyl acetate extract was subjected to bioassay-guided fractionation to ascertain the most active fraction, which was purified and identified using high-pressure liquid chromatography (HPLC) and GC-MS (Gas chromatography-Mass spectrometry) methods. Linoleic and linolenic acids identified from the ethyl acetate fraction showed IC50 of 6.88 μg/ml and 3.58 μg/ml, respectively. The study demonstrated greater antiplasmodial activity of the crude ethyl acetate extract of Carica papaya leaves with an IC50 of 2.96±0.14 μg/ml when compared to the activity of the fractions and isolated compounds. |
| format | Article |
| id | doaj-art-f58401606e624905a320521d49f8e655 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-f58401606e624905a320521d49f8e6552025-02-03T01:10:31ZengWileyJournal of Parasitology Research2090-00232090-00312011-01-01201110.1155/2011/104954104954Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya LeavesPaula Melariri0William Campbell1Paschal Etusim2Peter Smith3Division of Pharmacology, Department of Medicine, University of Cape Town Medical School K45, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South AfricaDivision of Pharmacology, Department of Medicine, University of Cape Town Medical School K45, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South AfricaDepartment of Animal and Environmental Biology, Abia State University Uturu, PMB 2000, Abia State, Uturu, NigeriaDivision of Pharmacology, Department of Medicine, University of Cape Town Medical School K45, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South AfricaWe investigated the antiplasmodial properties of crude extracts from Carica papaya leaves to trace the activity through bioassay-guided fractionation. The greatest antiplasmodial activity was observed in the ethyl acetate crude extract. C. papaya showed a high selectivity for P. falciparum against CHO cells with a selectivity index of 249.25 and 185.37 in the chloroquine-sensitive D10 and chloroquine-resistant DD2 strains, respectively. Carica papaya ethyl acetate extract was subjected to bioassay-guided fractionation to ascertain the most active fraction, which was purified and identified using high-pressure liquid chromatography (HPLC) and GC-MS (Gas chromatography-Mass spectrometry) methods. Linoleic and linolenic acids identified from the ethyl acetate fraction showed IC50 of 6.88 μg/ml and 3.58 μg/ml, respectively. The study demonstrated greater antiplasmodial activity of the crude ethyl acetate extract of Carica papaya leaves with an IC50 of 2.96±0.14 μg/ml when compared to the activity of the fractions and isolated compounds.http://dx.doi.org/10.1155/2011/104954 |
| spellingShingle | Paula Melariri William Campbell Paschal Etusim Peter Smith Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves Journal of Parasitology Research |
| title | Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves |
| title_full | Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves |
| title_fullStr | Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves |
| title_full_unstemmed | Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves |
| title_short | Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves |
| title_sort | antiplasmodial properties and bioassay guided fractionation of ethyl acetate extracts from carica papaya leaves |
| url | http://dx.doi.org/10.1155/2011/104954 |
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