An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp Buffaloes
Hemorrhagic septicemia (HS) is an important infectious disease in cattle and buffaloes, caused by Pasteurella multocida B:2 and E:2. The intranasal recombinant OmpH-based vaccine was successfully used to protect dairy cattle from HS in a previous study. Thus, this study aimed to examine the protecti...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2020/3548973 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567837517938688 |
|---|---|
| author | Anucha Muenthaisong Boondarika Nambooppha Amarin Rittipornlertrak Pallop Tankaew Thanya Varinrak Korkiat Muangthai Kheemchompu Atthikanyaphak Takuo Sawada Nattawooti Sthitmatee |
| author_facet | Anucha Muenthaisong Boondarika Nambooppha Amarin Rittipornlertrak Pallop Tankaew Thanya Varinrak Korkiat Muangthai Kheemchompu Atthikanyaphak Takuo Sawada Nattawooti Sthitmatee |
| author_sort | Anucha Muenthaisong |
| collection | DOAJ |
| description | Hemorrhagic septicemia (HS) is an important infectious disease in cattle and buffaloes, caused by Pasteurella multocida B:2 and E:2. The intranasal recombinant OmpH-based vaccine was successfully used to protect dairy cattle from HS in a previous study. Thus, this study aimed to examine the protective ability of that vaccine among buffaloes. Four groups of Thai swamp buffaloes received different vaccines and were labeled as 100 or 200 μg of the rOmpH with CpG-ODN2007, commercial HS bacterin vaccine, and nonvaccinated control groups. Sera and whole blood were collected to examine the antibody levels and cellular immune response using indirect ELISA and MTT assay, respectively. Challenge exposure was performed with virulent P. multocida strain M-1404 serotype B:2 on day 72 of the experiment. The antibody titers to P. multocida among immunized buffaloes were significantly higher than in the control group (p<0.01), especially the 200 μg of the rOmpH group. The stimulation index (SI) of the intranasally vaccinated groups revealed significantly higher levels than the nonvaccinated group (p<0.01), but not different from the intramuscularly commercial HS vaccine. The clinical signs and high fever were observed after challenge exposure in the nonvaccinated group, while it was not observed among the 200 μg of rOmpH immunized buffaloes. The other immunized groups showed partial protection with transient fever. In conclusion, the rOmpH-based intranasal vaccine could elicit protective ability and induce antibody- and cell-mediated immune response against virulent P. multocida strain among swamp buffaloes. |
| format | Article |
| id | doaj-art-f5752a4afa7c42a397b7c60905cd35a4 |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-f5752a4afa7c42a397b7c60905cd35a42025-02-03T01:00:28ZengWileyVeterinary Medicine International2090-81132042-00482020-01-01202010.1155/2020/35489733548973An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp BuffaloesAnucha Muenthaisong0Boondarika Nambooppha1Amarin Rittipornlertrak2Pallop Tankaew3Thanya Varinrak4Korkiat Muangthai5Kheemchompu Atthikanyaphak6Takuo Sawada7Nattawooti Sthitmatee8Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandCentral Laboratory, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandCentral Laboratory, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandBureau of Veterinary Biologics, Department of Livestock Developments, Ministry of Agriculture and Cooperative, Nakhon Ratchasima 30130, ThailandBureau of Veterinary Biologics, Department of Livestock Developments, Ministry of Agriculture and Cooperative, Nakhon Ratchasima 30130, ThailandLaboratory of Veterinary Microbiology, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, JapanDepartment of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandHemorrhagic septicemia (HS) is an important infectious disease in cattle and buffaloes, caused by Pasteurella multocida B:2 and E:2. The intranasal recombinant OmpH-based vaccine was successfully used to protect dairy cattle from HS in a previous study. Thus, this study aimed to examine the protective ability of that vaccine among buffaloes. Four groups of Thai swamp buffaloes received different vaccines and were labeled as 100 or 200 μg of the rOmpH with CpG-ODN2007, commercial HS bacterin vaccine, and nonvaccinated control groups. Sera and whole blood were collected to examine the antibody levels and cellular immune response using indirect ELISA and MTT assay, respectively. Challenge exposure was performed with virulent P. multocida strain M-1404 serotype B:2 on day 72 of the experiment. The antibody titers to P. multocida among immunized buffaloes were significantly higher than in the control group (p<0.01), especially the 200 μg of the rOmpH group. The stimulation index (SI) of the intranasally vaccinated groups revealed significantly higher levels than the nonvaccinated group (p<0.01), but not different from the intramuscularly commercial HS vaccine. The clinical signs and high fever were observed after challenge exposure in the nonvaccinated group, while it was not observed among the 200 μg of rOmpH immunized buffaloes. The other immunized groups showed partial protection with transient fever. In conclusion, the rOmpH-based intranasal vaccine could elicit protective ability and induce antibody- and cell-mediated immune response against virulent P. multocida strain among swamp buffaloes.http://dx.doi.org/10.1155/2020/3548973 |
| spellingShingle | Anucha Muenthaisong Boondarika Nambooppha Amarin Rittipornlertrak Pallop Tankaew Thanya Varinrak Korkiat Muangthai Kheemchompu Atthikanyaphak Takuo Sawada Nattawooti Sthitmatee An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp Buffaloes Veterinary Medicine International |
| title | An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp Buffaloes |
| title_full | An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp Buffaloes |
| title_fullStr | An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp Buffaloes |
| title_full_unstemmed | An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp Buffaloes |
| title_short | An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp Buffaloes |
| title_sort | intranasal vaccination with a recombinant outer membrane protein h against haemorrhagic septicemia in swamp buffaloes |
| url | http://dx.doi.org/10.1155/2020/3548973 |
| work_keys_str_mv | AT anuchamuenthaisong anintranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT boondarikanambooppha anintranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT amarinrittipornlertrak anintranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT palloptankaew anintranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT thanyavarinrak anintranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT korkiatmuangthai anintranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT kheemchompuatthikanyaphak anintranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT takuosawada anintranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT nattawootisthitmatee anintranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT anuchamuenthaisong intranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT boondarikanambooppha intranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT amarinrittipornlertrak intranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT palloptankaew intranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT thanyavarinrak intranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT korkiatmuangthai intranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT kheemchompuatthikanyaphak intranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT takuosawada intranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes AT nattawootisthitmatee intranasalvaccinationwitharecombinantoutermembraneproteinhagainsthaemorrhagicsepticemiainswampbuffaloes |