Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin
Two simple, accurate, and sensitive methods were developed for the determination of netobimin in the presence of its degradation product. Method (A) was an HPLC method, performed on C18 column using acetonitrile/methanol/0.01 M potassium dihydrogen phosphate (56 : 14 : 30 by volume) as a mobile phas...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/754650 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556029118775296 |
|---|---|
| author | Nesrin K. Ramadan Afaf O. Mohamed Sara E. Shawky Maissa Y. Salem |
| author_facet | Nesrin K. Ramadan Afaf O. Mohamed Sara E. Shawky Maissa Y. Salem |
| author_sort | Nesrin K. Ramadan |
| collection | DOAJ |
| description | Two simple, accurate, and sensitive methods were developed for the determination of netobimin in the presence of its degradation product. Method (A) was an HPLC method, performed on C18 column using acetonitrile/methanol/0.01 M potassium dihydrogen phosphate (56 : 14 : 30 by volume) as a mobile phase with a flow rate of 0.5 mL/min. Detection was performed at 254 nm. Method (B) was a TLC method, using silica gel 60 F254 plates; the optimized mobile phase was toluene/methanol/chloroform/ammonium hydroxide (5 : 4 : 6 : 0.1 by volume). The spots were scanned densitometrically at 346 nm. Linearity ranges were 1–10 μg/mL for method (A) and 0.5–5 μg/band for method (B), and the mean percentage recoveries were 99.3±0.7% and 99.7±0.7% for methods (A) and (B), respectively. The proposed methods were found to be specific for netobimin in the presence of up to 90% of its degradation product. Statistical comparison between the results obtained by these methods and the manufacturer method was done, and no significance difference was obtained. |
| format | Article |
| id | doaj-art-f574f93ba3a24969bfd83986cc9131d4 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-f574f93ba3a24969bfd83986cc9131d42025-02-03T05:46:38ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732012-01-01201210.1155/2012/754650754650Different Stability-Indicating Chromatographic Techniques for the Determination of NetobiminNesrin K. Ramadan0Afaf O. Mohamed1Sara E. Shawky2Maissa Y. Salem3Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Giza 11562, EgyptAnalytical Chemistry Department, National Organization for Drug Control and Research (NODCAR), 6 Abu Hazem Street, Pyramids Avenue, P.O. Box 29, 35521, EgyptAnalytical Chemistry Department, National Organization for Drug Control and Research (NODCAR), 6 Abu Hazem Street, Pyramids Avenue, P.O. Box 29, 35521, EgyptAnalytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Giza 11562, EgyptTwo simple, accurate, and sensitive methods were developed for the determination of netobimin in the presence of its degradation product. Method (A) was an HPLC method, performed on C18 column using acetonitrile/methanol/0.01 M potassium dihydrogen phosphate (56 : 14 : 30 by volume) as a mobile phase with a flow rate of 0.5 mL/min. Detection was performed at 254 nm. Method (B) was a TLC method, using silica gel 60 F254 plates; the optimized mobile phase was toluene/methanol/chloroform/ammonium hydroxide (5 : 4 : 6 : 0.1 by volume). The spots were scanned densitometrically at 346 nm. Linearity ranges were 1–10 μg/mL for method (A) and 0.5–5 μg/band for method (B), and the mean percentage recoveries were 99.3±0.7% and 99.7±0.7% for methods (A) and (B), respectively. The proposed methods were found to be specific for netobimin in the presence of up to 90% of its degradation product. Statistical comparison between the results obtained by these methods and the manufacturer method was done, and no significance difference was obtained.http://dx.doi.org/10.1155/2012/754650 |
| spellingShingle | Nesrin K. Ramadan Afaf O. Mohamed Sara E. Shawky Maissa Y. Salem Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin Journal of Analytical Methods in Chemistry |
| title | Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin |
| title_full | Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin |
| title_fullStr | Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin |
| title_full_unstemmed | Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin |
| title_short | Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin |
| title_sort | different stability indicating chromatographic techniques for the determination of netobimin |
| url | http://dx.doi.org/10.1155/2012/754650 |
| work_keys_str_mv | AT nesrinkramadan differentstabilityindicatingchromatographictechniquesforthedeterminationofnetobimin AT afafomohamed differentstabilityindicatingchromatographictechniquesforthedeterminationofnetobimin AT saraeshawky differentstabilityindicatingchromatographictechniquesforthedeterminationofnetobimin AT maissaysalem differentstabilityindicatingchromatographictechniquesforthedeterminationofnetobimin |