Proteomics and Microbiota Conjoint Analysis in the Nasal Mucus: Revelation of Differences in Immunological Function in <i>Manis javanica</i> and <i>Manis pentadactyla</i>

All eight pangolin species, especially captive <i>Manis pentadactyla</i>, are critically endangered and susceptible to various pathogenic microorganisms, causing mass mortality. They are involved in the complement system, iron transport system, and inflammatory factors. <i>M. penta...

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Main Authors: Qing Han, Yepin Yu, Hongbin Sun, Xiujuan Zhang, Ping Liu, Jianfeng Deng, Xinyuan Hu, Jinping Chen
Format: Article
Language:English
Published: MDPI AG 2024-09-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/14/18/2683
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author Qing Han
Yepin Yu
Hongbin Sun
Xiujuan Zhang
Ping Liu
Jianfeng Deng
Xinyuan Hu
Jinping Chen
author_facet Qing Han
Yepin Yu
Hongbin Sun
Xiujuan Zhang
Ping Liu
Jianfeng Deng
Xinyuan Hu
Jinping Chen
author_sort Qing Han
collection DOAJ
description All eight pangolin species, especially captive <i>Manis pentadactyla</i>, are critically endangered and susceptible to various pathogenic microorganisms, causing mass mortality. They are involved in the complement system, iron transport system, and inflammatory factors. <i>M. pentadactyla</i> exhibited a higher abundance of opportunistic pathogens, <i>Moraxella</i>, which potentially evaded complement-mediated immune response by reducing C5 levels and counteracting detrimental effects through transferrin neutralization. In addition, we found that the major structure of C5a, an important inflammatory factor, was lacking in <i>M. javanica</i>. In brief, this study revealed the differences in immune factors and microbiome between <i>M. javanica</i> and <i>M. pentadactyla</i>, thus providing a theoretical basis for subsequent immunotherapy.
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id doaj-art-f536c88b2ee14cd39d2775d26c16f5d2
institution OA Journals
issn 2076-2615
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publishDate 2024-09-01
publisher MDPI AG
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series Animals
spelling doaj-art-f536c88b2ee14cd39d2775d26c16f5d22025-08-20T01:56:04ZengMDPI AGAnimals2076-26152024-09-011418268310.3390/ani14182683Proteomics and Microbiota Conjoint Analysis in the Nasal Mucus: Revelation of Differences in Immunological Function in <i>Manis javanica</i> and <i>Manis pentadactyla</i>Qing Han0Yepin Yu1Hongbin Sun2Xiujuan Zhang3Ping Liu4Jianfeng Deng5Xinyuan Hu6Jinping Chen7Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaShenzhen Natural Reserve Management Center, Shenzhen 518115, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaShenzhen Natural Reserve Management Center, Shenzhen 518115, ChinaShenzhen Natural Reserve Management Center, Shenzhen 518115, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaAll eight pangolin species, especially captive <i>Manis pentadactyla</i>, are critically endangered and susceptible to various pathogenic microorganisms, causing mass mortality. They are involved in the complement system, iron transport system, and inflammatory factors. <i>M. pentadactyla</i> exhibited a higher abundance of opportunistic pathogens, <i>Moraxella</i>, which potentially evaded complement-mediated immune response by reducing C5 levels and counteracting detrimental effects through transferrin neutralization. In addition, we found that the major structure of C5a, an important inflammatory factor, was lacking in <i>M. javanica</i>. In brief, this study revealed the differences in immune factors and microbiome between <i>M. javanica</i> and <i>M. pentadactyla</i>, thus providing a theoretical basis for subsequent immunotherapy.https://www.mdpi.com/2076-2615/14/18/2683<i>Manis javanica</i><i>Manis pentadactyla</i>nasal mucosa microbiotaTMT-proteomics
spellingShingle Qing Han
Yepin Yu
Hongbin Sun
Xiujuan Zhang
Ping Liu
Jianfeng Deng
Xinyuan Hu
Jinping Chen
Proteomics and Microbiota Conjoint Analysis in the Nasal Mucus: Revelation of Differences in Immunological Function in <i>Manis javanica</i> and <i>Manis pentadactyla</i>
Animals
<i>Manis javanica</i>
<i>Manis pentadactyla</i>
nasal mucosa microbiota
TMT-proteomics
title Proteomics and Microbiota Conjoint Analysis in the Nasal Mucus: Revelation of Differences in Immunological Function in <i>Manis javanica</i> and <i>Manis pentadactyla</i>
title_full Proteomics and Microbiota Conjoint Analysis in the Nasal Mucus: Revelation of Differences in Immunological Function in <i>Manis javanica</i> and <i>Manis pentadactyla</i>
title_fullStr Proteomics and Microbiota Conjoint Analysis in the Nasal Mucus: Revelation of Differences in Immunological Function in <i>Manis javanica</i> and <i>Manis pentadactyla</i>
title_full_unstemmed Proteomics and Microbiota Conjoint Analysis in the Nasal Mucus: Revelation of Differences in Immunological Function in <i>Manis javanica</i> and <i>Manis pentadactyla</i>
title_short Proteomics and Microbiota Conjoint Analysis in the Nasal Mucus: Revelation of Differences in Immunological Function in <i>Manis javanica</i> and <i>Manis pentadactyla</i>
title_sort proteomics and microbiota conjoint analysis in the nasal mucus revelation of differences in immunological function in i manis javanica i and i manis pentadactyla i
topic <i>Manis javanica</i>
<i>Manis pentadactyla</i>
nasal mucosa microbiota
TMT-proteomics
url https://www.mdpi.com/2076-2615/14/18/2683
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