Pan-cancer analysis and single-cell analysis identifies the CENPN as a biomarker for survival prognosis and immunotherapy
Abstract Background Centromere protein N (CENPN), located on chromosome 16q23.2, encodes vital nucleosome-associated complexes that are essential for dynamic assembly processes. CENPN plays a pivotal role in regulating cell proliferation and cell cycle progression by influencing mitotic events. Desp...
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-01801-2 |
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| _version_ | 1832585678261583872 |
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| author | Nie Zhang Zhuoying He Xuejin Qin Ke Han Zhengchun Zhu Fei Zhong |
| author_facet | Nie Zhang Zhuoying He Xuejin Qin Ke Han Zhengchun Zhu Fei Zhong |
| author_sort | Nie Zhang |
| collection | DOAJ |
| description | Abstract Background Centromere protein N (CENPN), located on chromosome 16q23.2, encodes vital nucleosome-associated complexes that are essential for dynamic assembly processes. CENPN plays a pivotal role in regulating cell proliferation and cell cycle progression by influencing mitotic events. Despite its potential importance, the precise functional role and regulatory mechanisms of CENPN in diverse malignancies remain largely unexplored. This study aimed to elucidate the role of CENPN in human cancers and evaluate its prognostic significance. Methods Investigate the role of CENPN in various malignancies, we leveraged data from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases. We employed a comprehensive suite of web platforms and software tools for data analysis, including R, Cytoscape, an integrated repository portal for tumor-immune system interactions (TISIDB), CBio Cancer Genomics Portal (cBioPortal), Search Tool for the Retrieval of Interaction Gene/Proteins (STRING), Gene Set Cancer Analysis (GSCALite), and a cancer single-cell state atlas (CancerSEA). Results The findings demonstrated that CENPN expression was elevated in the majority of cancer types and differentially expressed across molecular and immune subtypes. Functional enrichment analysis in multiple tumors also identified possible pathways of CENPN involvement in tumorigenesis. Its expression positively correlated with Th2 and Tcm cells in most cancers. It is also correlated with genetic markers of immunomodulators in various cancers. Conclusions Overall, CENPN expression is closely related to cancers and has the potential to act as a cancer biomarker. |
| format | Article |
| id | doaj-art-f530d4356eeb4e1a973426f9effc9be8 |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-f530d4356eeb4e1a973426f9effc9be82025-01-26T12:39:52ZengSpringerDiscover Oncology2730-60112025-01-0116112510.1007/s12672-025-01801-2Pan-cancer analysis and single-cell analysis identifies the CENPN as a biomarker for survival prognosis and immunotherapyNie Zhang0Zhuoying He1Xuejin Qin2Ke Han3Zhengchun Zhu4Fei Zhong5Department of Oncology, Fuyang Hospital of Anhui Medical UniversityDepartment of Oncology, Fuyang Hospital of Anhui Medical UniversityDepartment of Oncology, Fuyang Hospital of Anhui Medical UniversityDepartment of Oncology, Fuyang Hospital of Anhui Medical UniversityDepartment of Oncology, Fuyang Hospital of Anhui Medical UniversityDepartment of Oncology, Fuyang Hospital of Anhui Medical UniversityAbstract Background Centromere protein N (CENPN), located on chromosome 16q23.2, encodes vital nucleosome-associated complexes that are essential for dynamic assembly processes. CENPN plays a pivotal role in regulating cell proliferation and cell cycle progression by influencing mitotic events. Despite its potential importance, the precise functional role and regulatory mechanisms of CENPN in diverse malignancies remain largely unexplored. This study aimed to elucidate the role of CENPN in human cancers and evaluate its prognostic significance. Methods Investigate the role of CENPN in various malignancies, we leveraged data from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases. We employed a comprehensive suite of web platforms and software tools for data analysis, including R, Cytoscape, an integrated repository portal for tumor-immune system interactions (TISIDB), CBio Cancer Genomics Portal (cBioPortal), Search Tool for the Retrieval of Interaction Gene/Proteins (STRING), Gene Set Cancer Analysis (GSCALite), and a cancer single-cell state atlas (CancerSEA). Results The findings demonstrated that CENPN expression was elevated in the majority of cancer types and differentially expressed across molecular and immune subtypes. Functional enrichment analysis in multiple tumors also identified possible pathways of CENPN involvement in tumorigenesis. Its expression positively correlated with Th2 and Tcm cells in most cancers. It is also correlated with genetic markers of immunomodulators in various cancers. Conclusions Overall, CENPN expression is closely related to cancers and has the potential to act as a cancer biomarker.https://doi.org/10.1007/s12672-025-01801-2CENPNPan-cancerPrognosisImmune infiltrationTumor microenvironment |
| spellingShingle | Nie Zhang Zhuoying He Xuejin Qin Ke Han Zhengchun Zhu Fei Zhong Pan-cancer analysis and single-cell analysis identifies the CENPN as a biomarker for survival prognosis and immunotherapy Discover Oncology CENPN Pan-cancer Prognosis Immune infiltration Tumor microenvironment |
| title | Pan-cancer analysis and single-cell analysis identifies the CENPN as a biomarker for survival prognosis and immunotherapy |
| title_full | Pan-cancer analysis and single-cell analysis identifies the CENPN as a biomarker for survival prognosis and immunotherapy |
| title_fullStr | Pan-cancer analysis and single-cell analysis identifies the CENPN as a biomarker for survival prognosis and immunotherapy |
| title_full_unstemmed | Pan-cancer analysis and single-cell analysis identifies the CENPN as a biomarker for survival prognosis and immunotherapy |
| title_short | Pan-cancer analysis and single-cell analysis identifies the CENPN as a biomarker for survival prognosis and immunotherapy |
| title_sort | pan cancer analysis and single cell analysis identifies the cenpn as a biomarker for survival prognosis and immunotherapy |
| topic | CENPN Pan-cancer Prognosis Immune infiltration Tumor microenvironment |
| url | https://doi.org/10.1007/s12672-025-01801-2 |
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