Differential Effects of Alpha-Particle Radiation and X-Irradiation on Genes Associated with Apoptosis
This study examined differential effects of alpha-(α-) particle radiation and X-rays on apoptosis and associated changes in gene expression. Human monocytic cells were exposed to α-particle radiation and X-rays from 0 to 1.5 Gy. Four days postexposure, cell death was measured by flow cytometry and...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Radiology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2011/679806 |
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| _version_ | 1832568399802138624 |
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| author | Vinita Chauhan Matthew Howland Jeremy Chen Barbara Kutzner Ruth C. Wilkins |
| author_facet | Vinita Chauhan Matthew Howland Jeremy Chen Barbara Kutzner Ruth C. Wilkins |
| author_sort | Vinita Chauhan |
| collection | DOAJ |
| description | This study examined differential effects of alpha-(α-) particle radiation and X-rays on apoptosis and associated changes in gene expression. Human monocytic cells were exposed to α-particle radiation and X-rays from 0 to 1.5 Gy. Four days postexposure, cell death was measured by flow cytometry and 84 genes related to apoptosis were analyzed using real-time PCR. On average, 33% of the cells were apoptotic at 1.5 Gy of α-particle radiation. Transcript profiling showed statistical expression of 15 genes at all three doses tested. Cells exposed to X-rays were <5% apoptotic at ~1.5 Gy and induced less than a 2-fold expression in 6 apoptotic genes at the higher doses of radiation. Among these 6 genes, Fas and TNF-α were common to the α-irradiated cells. This data suggests that α-particle radiation initiates cell death by TNF-α and Fas activation and through intermediate signalling mediators that are distinct from X-irradiated cells. |
| format | Article |
| id | doaj-art-f51a192fbcf7418586af4c93fb08edb9 |
| institution | Kabale University |
| issn | 2090-1941 2090-195X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Radiology Research and Practice |
| spelling | doaj-art-f51a192fbcf7418586af4c93fb08edb92025-02-03T00:59:14ZengWileyRadiology Research and Practice2090-19412090-195X2011-01-01201110.1155/2011/679806679806Differential Effects of Alpha-Particle Radiation and X-Irradiation on Genes Associated with ApoptosisVinita Chauhan0Matthew Howland1Jeremy Chen2Barbara Kutzner3Ruth C. Wilkins4Consumer and Clinical Radiation Protection Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, 775 Brookfield Road, PL 6303B, Ottawa, ON, K1A 0K9, CanadaConsumer and Clinical Radiation Protection Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, 775 Brookfield Road, PL 6303B, Ottawa, ON, K1A 0K9, CanadaConsumer and Clinical Radiation Protection Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, 775 Brookfield Road, PL 6303B, Ottawa, ON, K1A 0K9, CanadaConsumer and Clinical Radiation Protection Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, 775 Brookfield Road, PL 6303B, Ottawa, ON, K1A 0K9, CanadaConsumer and Clinical Radiation Protection Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, 775 Brookfield Road, PL 6303B, Ottawa, ON, K1A 0K9, CanadaThis study examined differential effects of alpha-(α-) particle radiation and X-rays on apoptosis and associated changes in gene expression. Human monocytic cells were exposed to α-particle radiation and X-rays from 0 to 1.5 Gy. Four days postexposure, cell death was measured by flow cytometry and 84 genes related to apoptosis were analyzed using real-time PCR. On average, 33% of the cells were apoptotic at 1.5 Gy of α-particle radiation. Transcript profiling showed statistical expression of 15 genes at all three doses tested. Cells exposed to X-rays were <5% apoptotic at ~1.5 Gy and induced less than a 2-fold expression in 6 apoptotic genes at the higher doses of radiation. Among these 6 genes, Fas and TNF-α were common to the α-irradiated cells. This data suggests that α-particle radiation initiates cell death by TNF-α and Fas activation and through intermediate signalling mediators that are distinct from X-irradiated cells.http://dx.doi.org/10.1155/2011/679806 |
| spellingShingle | Vinita Chauhan Matthew Howland Jeremy Chen Barbara Kutzner Ruth C. Wilkins Differential Effects of Alpha-Particle Radiation and X-Irradiation on Genes Associated with Apoptosis Radiology Research and Practice |
| title | Differential Effects of Alpha-Particle Radiation and X-Irradiation on Genes Associated with Apoptosis |
| title_full | Differential Effects of Alpha-Particle Radiation and X-Irradiation on Genes Associated with Apoptosis |
| title_fullStr | Differential Effects of Alpha-Particle Radiation and X-Irradiation on Genes Associated with Apoptosis |
| title_full_unstemmed | Differential Effects of Alpha-Particle Radiation and X-Irradiation on Genes Associated with Apoptosis |
| title_short | Differential Effects of Alpha-Particle Radiation and X-Irradiation on Genes Associated with Apoptosis |
| title_sort | differential effects of alpha particle radiation and x irradiation on genes associated with apoptosis |
| url | http://dx.doi.org/10.1155/2011/679806 |
| work_keys_str_mv | AT vinitachauhan differentialeffectsofalphaparticleradiationandxirradiationongenesassociatedwithapoptosis AT matthewhowland differentialeffectsofalphaparticleradiationandxirradiationongenesassociatedwithapoptosis AT jeremychen differentialeffectsofalphaparticleradiationandxirradiationongenesassociatedwithapoptosis AT barbarakutzner differentialeffectsofalphaparticleradiationandxirradiationongenesassociatedwithapoptosis AT ruthcwilkins differentialeffectsofalphaparticleradiationandxirradiationongenesassociatedwithapoptosis |