Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell Carcinoma
The development of esophageal squamous cell carcinoma (ESCC) is poorly understood and the major regulatory molecules involved in the process of tumorigenesis have not yet been identified. We had previously employed a quantitative proteomic approach to identify differentially expressed proteins in ES...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2015/325721 |
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| author | Harsh Pawar Srinivas M. Srikanth Manoj Kumar Kashyap Gajanan Sathe Sandip Chavan Mukul Singal H. C. Manju Kariyanakatte Veeraiah Veerendra Kumar M. Vijayakumar Ravi Sirdeshmukh Akhilesh Pandey T. S. Keshava Prasad Harsha Gowda Rekha V. Kumar |
| author_facet | Harsh Pawar Srinivas M. Srikanth Manoj Kumar Kashyap Gajanan Sathe Sandip Chavan Mukul Singal H. C. Manju Kariyanakatte Veeraiah Veerendra Kumar M. Vijayakumar Ravi Sirdeshmukh Akhilesh Pandey T. S. Keshava Prasad Harsha Gowda Rekha V. Kumar |
| author_sort | Harsh Pawar |
| collection | DOAJ |
| description | The development of esophageal squamous cell carcinoma (ESCC) is poorly understood and the major regulatory molecules involved in the process of tumorigenesis have not yet been identified. We had previously employed a quantitative proteomic approach to identify differentially expressed proteins in ESCC tumors. A total of 238 differentially expressed proteins were identified in that study including S100 calcium binding protein A9 (S100A9) as one of the major downregulated proteins. In the present study, we carried out immunohistochemical validation of S100A9 in a large cohort of ESCC patients to determine the expression and subcellular localization of S100A9 in tumors and adjacent normal esophageal epithelia. Downregulation of S100A9 was observed in 67% (n=192) of 288 different ESCC tumors, with the most dramatic downregulation observed in the poorly differentiated tumors (99/111). Expression of S100A9 was restricted to the prickle and functional layers of normal esophageal mucosa and localized predominantly in the cytoplasm and nucleus whereas virtually no expression was observed in the tumor and stromal cells. This suggests the important role that S100A9 plays in maintaining the differentiated state of epithelium and suggests that its downregulation may be associated with increased susceptibility to tumor formation. |
| format | Article |
| id | doaj-art-f4f67f27f3be4d468921bba57719d38a |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-f4f67f27f3be4d468921bba57719d38a2025-02-03T01:21:30ZengWileyThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/325721325721Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell CarcinomaHarsh Pawar0Srinivas M. Srikanth1Manoj Kumar Kashyap2Gajanan Sathe3Sandip Chavan4Mukul Singal5H. C. Manju6Kariyanakatte Veeraiah Veerendra Kumar7M. Vijayakumar8Ravi Sirdeshmukh9Akhilesh Pandey10T. S. Keshava Prasad11Harsha Gowda12Rekha V. Kumar13Institute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaGovernment Medical College and Hospital, Sector 32, Chandigarh 160030, IndiaDepartment of Pathology, Kidwai Memorial Institute of Oncology, Bangalore 560029, IndiaDepartment of Surgical Oncology, Kidwai Memorial Institute of Oncology, Bangalore 560029, IndiaDepartment of Surgical Oncology, Kidwai Memorial Institute of Oncology, Bangalore 560029, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaMcKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USAInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaDepartment of Pathology, Kidwai Memorial Institute of Oncology, Bangalore 560029, IndiaThe development of esophageal squamous cell carcinoma (ESCC) is poorly understood and the major regulatory molecules involved in the process of tumorigenesis have not yet been identified. We had previously employed a quantitative proteomic approach to identify differentially expressed proteins in ESCC tumors. A total of 238 differentially expressed proteins were identified in that study including S100 calcium binding protein A9 (S100A9) as one of the major downregulated proteins. In the present study, we carried out immunohistochemical validation of S100A9 in a large cohort of ESCC patients to determine the expression and subcellular localization of S100A9 in tumors and adjacent normal esophageal epithelia. Downregulation of S100A9 was observed in 67% (n=192) of 288 different ESCC tumors, with the most dramatic downregulation observed in the poorly differentiated tumors (99/111). Expression of S100A9 was restricted to the prickle and functional layers of normal esophageal mucosa and localized predominantly in the cytoplasm and nucleus whereas virtually no expression was observed in the tumor and stromal cells. This suggests the important role that S100A9 plays in maintaining the differentiated state of epithelium and suggests that its downregulation may be associated with increased susceptibility to tumor formation.http://dx.doi.org/10.1155/2015/325721 |
| spellingShingle | Harsh Pawar Srinivas M. Srikanth Manoj Kumar Kashyap Gajanan Sathe Sandip Chavan Mukul Singal H. C. Manju Kariyanakatte Veeraiah Veerendra Kumar M. Vijayakumar Ravi Sirdeshmukh Akhilesh Pandey T. S. Keshava Prasad Harsha Gowda Rekha V. Kumar Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell Carcinoma The Scientific World Journal |
| title | Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell Carcinoma |
| title_full | Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell Carcinoma |
| title_fullStr | Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell Carcinoma |
| title_full_unstemmed | Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell Carcinoma |
| title_short | Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell Carcinoma |
| title_sort | downregulation of s100 calcium binding protein a9 in esophageal squamous cell carcinoma |
| url | http://dx.doi.org/10.1155/2015/325721 |
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