Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin
Lead (Pb2+) exposure continues to be a significant public health problem. Therefore, it is vital to have a continuous epidemiological dataset for a better understanding of Pb2+ toxicity. In the present study, we have exposed stem cells isolated from deciduous and permanent teeth, periodontal ligamen...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/235941 |
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| author | Mariam Abdullah Fazliny Abd. Rahman Nareshwaran Gnanasegaran Vijayendran Govindasamy Noor Hayaty Abu Kasim Sabri Musa |
| author_facet | Mariam Abdullah Fazliny Abd. Rahman Nareshwaran Gnanasegaran Vijayendran Govindasamy Noor Hayaty Abu Kasim Sabri Musa |
| author_sort | Mariam Abdullah |
| collection | DOAJ |
| description | Lead (Pb2+) exposure continues to be a significant public health problem. Therefore, it is vital to have a continuous epidemiological dataset for a better understanding of Pb2+ toxicity. In the present study, we have exposed stem cells isolated from deciduous and permanent teeth, periodontal ligament, and bone marrow to five different types of Pb2+ concentrations (160, 80, 40, 20, and 10 µM) for 24 hours to identify the adverse effects of Pb2+ on the proliferation, differentiation, and gene expression on these cell lines. We found that Pb2+ treatment altered the morphology and adhesion of the cells in a dose-dependent manner. There were no significant changes in terms of cell surface phenotypes. Cells exposed to Pb2+ continued to differentiate into chondrogenesis and adipogenesis, and a severe downregulation was observed in osteogenesis. Gene expression studies revealed a constant expression of key markers associated with stemness (Oct 4, Rex 1) and DNA repair enzyme markers, but downregulation occurred with some ectoderm and endoderm markers, demonstrating an irregular and untimely differentiation trail. Our study revealed for the first time that Pb2+ exposure not only affects the phenotypic characteristics but also induces significant alteration in the differentiation and gene expression in the cells. |
| format | Article |
| id | doaj-art-f4ee7833ba08462f8970d99dbd47b498 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-f4ee7833ba08462f8970d99dbd47b4982025-02-03T01:28:41ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/235941235941Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental OriginMariam Abdullah0Fazliny Abd. Rahman1Nareshwaran Gnanasegaran2Vijayendran Govindasamy3Noor Hayaty Abu Kasim4Sabri Musa5Department of Conservative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Conservative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Conservative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaHygieia Innovation Sdn. Bhd, Lot 1G-2G, Komplex Lanai, No. 2, Persiaran Seri Perdana, Persint 10, 62250 Putrajaya, MalaysiaDepartment of Conservative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Children’s Dentistry and Orthodontics, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaLead (Pb2+) exposure continues to be a significant public health problem. Therefore, it is vital to have a continuous epidemiological dataset for a better understanding of Pb2+ toxicity. In the present study, we have exposed stem cells isolated from deciduous and permanent teeth, periodontal ligament, and bone marrow to five different types of Pb2+ concentrations (160, 80, 40, 20, and 10 µM) for 24 hours to identify the adverse effects of Pb2+ on the proliferation, differentiation, and gene expression on these cell lines. We found that Pb2+ treatment altered the morphology and adhesion of the cells in a dose-dependent manner. There were no significant changes in terms of cell surface phenotypes. Cells exposed to Pb2+ continued to differentiate into chondrogenesis and adipogenesis, and a severe downregulation was observed in osteogenesis. Gene expression studies revealed a constant expression of key markers associated with stemness (Oct 4, Rex 1) and DNA repair enzyme markers, but downregulation occurred with some ectoderm and endoderm markers, demonstrating an irregular and untimely differentiation trail. Our study revealed for the first time that Pb2+ exposure not only affects the phenotypic characteristics but also induces significant alteration in the differentiation and gene expression in the cells.http://dx.doi.org/10.1155/2014/235941 |
| spellingShingle | Mariam Abdullah Fazliny Abd. Rahman Nareshwaran Gnanasegaran Vijayendran Govindasamy Noor Hayaty Abu Kasim Sabri Musa Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin The Scientific World Journal |
| title | Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin |
| title_full | Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin |
| title_fullStr | Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin |
| title_full_unstemmed | Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin |
| title_short | Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin |
| title_sort | diverse effects of lead nitrate on the proliferation differentiation and gene expression of stem cells isolated from a dental origin |
| url | http://dx.doi.org/10.1155/2014/235941 |
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