Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPARα
The interleukin-10 gene-deficient (Il10-/-) mouse is a model of human inflammatory bowel disease and Ppara has been identified as one of the key genes involved in regulation of colitis in the bacterially inoculated Il10-/- model. The aims were to (1) characterize colitis onset and progression using...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2010/621069 |
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| author | Bianca Knoch Matthew P. G. Barnett Janine Cooney Warren C. McNabb Diane Barraclough William Laing Shuotun Zhu Zaneta A. Park Paul MacLean Scott O. Knowles Nicole C. Roy |
| author_facet | Bianca Knoch Matthew P. G. Barnett Janine Cooney Warren C. McNabb Diane Barraclough William Laing Shuotun Zhu Zaneta A. Park Paul MacLean Scott O. Knowles Nicole C. Roy |
| author_sort | Bianca Knoch |
| collection | DOAJ |
| description | The interleukin-10 gene-deficient (Il10-/-) mouse is a model of human inflammatory bowel disease and Ppara has been identified as one of the key genes involved in regulation of colitis in the bacterially inoculated Il10-/- model. The aims were to (1) characterize colitis onset and progression using a histopathological, transcriptomic, and proteomic approach and (2) investigate links between PPARα and IL10 using gene network analysis. Bacterial inoculation resulted in severe colitis in Il10-/- mice from 10 to 12 weeks of age. Innate and adaptive immune responses showed differences in gene expression relating to colitis severity. Actin cytoskeleton dynamics, innate immunity, and apoptosis-linked gene and protein expression data suggested a delayed remodeling process in 12-week-old Il10-/- mice. Gene expression changes in 12-week-old Il10-/- mice were related to PPARα signaling likely to control colitis, but how PPARα activation might regulate intestinal IL10 production remains to be determined. |
| format | Article |
| id | doaj-art-f4bb7c9d42804d6c97005aad1d4d4762 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-f4bb7c9d42804d6c97005aad1d4d47622025-02-03T01:09:45ZengWileyPPAR Research1687-47571687-47652010-01-01201010.1155/2010/621069621069Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPARαBianca Knoch0Matthew P. G. Barnett1Janine Cooney2Warren C. McNabb3Diane Barraclough4William Laing5Shuotun Zhu6Zaneta A. Park7Paul MacLean8Scott O. Knowles9Nicole C. Roy10Food, Metabolism & Microbiology Section, Food & Textiles Group, AgResearch Grasslands, Tennent Drive, Palmerston North 4442, New ZealandFood, Metabolism & Microbiology Section, Food & Textiles Group, AgResearch Grasslands, Tennent Drive, Palmerston North 4442, New ZealandPlant & Food Research, East Street, Hamilton 3214, and 120 Mt Albert Road, Sandringham, Auckland 1025, New ZealandFood & Textiles Group, AgResearch Grasslands, Tennent Drive, Palmerston North 4442, New ZealandPlant & Food Research, East Street, Hamilton 3214, and 120 Mt Albert Road, Sandringham, Auckland 1025, New ZealandPlant & Food Research, East Street, Hamilton 3214, and 120 Mt Albert Road, Sandringham, Auckland 1025, New ZealandFaculty of Medical and Health Sciences, The University of Auckland, 85 Park Road, Grafton, Auckland 1023, New ZealandBioinformatics, Mathematics & Statistics Section, AgResearch Grasslands, Tennent Drive, Palmerston North 4442, and AgResearch Ruakura, East Street, Hamilton 3240, New ZealandBioinformatics, Mathematics & Statistics Section, AgResearch Grasslands, Tennent Drive, Palmerston North 4442, and AgResearch Ruakura, East Street, Hamilton 3240, New ZealandFood, Metabolism & Microbiology Section, Food & Textiles Group, AgResearch Grasslands, Tennent Drive, Palmerston North 4442, New ZealandFood, Metabolism & Microbiology Section, Food & Textiles Group, AgResearch Grasslands, Tennent Drive, Palmerston North 4442, New ZealandThe interleukin-10 gene-deficient (Il10-/-) mouse is a model of human inflammatory bowel disease and Ppara has been identified as one of the key genes involved in regulation of colitis in the bacterially inoculated Il10-/- model. The aims were to (1) characterize colitis onset and progression using a histopathological, transcriptomic, and proteomic approach and (2) investigate links between PPARα and IL10 using gene network analysis. Bacterial inoculation resulted in severe colitis in Il10-/- mice from 10 to 12 weeks of age. Innate and adaptive immune responses showed differences in gene expression relating to colitis severity. Actin cytoskeleton dynamics, innate immunity, and apoptosis-linked gene and protein expression data suggested a delayed remodeling process in 12-week-old Il10-/- mice. Gene expression changes in 12-week-old Il10-/- mice were related to PPARα signaling likely to control colitis, but how PPARα activation might regulate intestinal IL10 production remains to be determined.http://dx.doi.org/10.1155/2010/621069 |
| spellingShingle | Bianca Knoch Matthew P. G. Barnett Janine Cooney Warren C. McNabb Diane Barraclough William Laing Shuotun Zhu Zaneta A. Park Paul MacLean Scott O. Knowles Nicole C. Roy Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPARα PPAR Research |
| title | Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPARα |
| title_full | Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPARα |
| title_fullStr | Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPARα |
| title_full_unstemmed | Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPARα |
| title_short | Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPARα |
| title_sort | molecular characterization of the onset and progression of colitis in inoculated interleukin 10 gene deficient mice a role for pparα |
| url | http://dx.doi.org/10.1155/2010/621069 |
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