Genetic Variant of C-5434T REN Enhancer on Serum Renin Levels and Binding Pattern of Signal Transducers and Activators Transcription 3
The human renin gene has been widely known to be involved in essential hypertension (EH) pathogenesis. Genetic variant C-5434T of REN enhancer contributed to renin gene transcription and serum renin regulation. However, the mechanism associated with the transcription level changes remains unknown, a...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Hypertension |
| Online Access: | http://dx.doi.org/10.1155/2015/486961 |
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| author | Imama Maslahah Mohammad Saifur Rohman Nashi Widodo Agustina Tri Endharti Didik Utomo |
| author_facet | Imama Maslahah Mohammad Saifur Rohman Nashi Widodo Agustina Tri Endharti Didik Utomo |
| author_sort | Imama Maslahah |
| collection | DOAJ |
| description | The human renin gene has been widely known to be involved in essential hypertension (EH) pathogenesis. Genetic variant C-5434T of REN enhancer contributed to renin gene transcription and serum renin regulation. However, the mechanism associated with the transcription level changes remains unknown, and only a few reports exist that discussed serum renin levels on C-5434T of REN. Thus, this study aims to investigate the relationship between genetic variant C-5434T of REN enhancer and serum renin levels in Indonesian hypertensive patients. SNP of C-5434T was genotyped in 56 hypertensive patients by using RFLP. The data showed that serum renin is slightly higher in hypertensive patients with the TT genotype (39 ± 10.3) than patients with the CC genotype (33 ± 10.6) but the difference was not statistically significant (p=0.35). Here, we also present a docking approach for predicting interaction between genetic variant -5434C/T and STAT3 (Signal Transducers and Activators Transcription 3), the predicted transcription factor that regulates renin gene enhancer. The results showed that STAT3-DNA allele T more favorably binds to DNA than STAT3-DNA allele C. These data suggest that the presence of genetic variant C-5434T has changed the binding pattern of STAT3 to REN enhancer. This is likely to influence STAT3 activity to stimulate the expression of renin gene in producing renin. |
| format | Article |
| id | doaj-art-f4b89649879142288456dd7b523cea4f |
| institution | Kabale University |
| issn | 2090-0384 2090-0392 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
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| series | International Journal of Hypertension |
| spelling | doaj-art-f4b89649879142288456dd7b523cea4f2025-02-03T01:07:44ZengWileyInternational Journal of Hypertension2090-03842090-03922015-01-01201510.1155/2015/486961486961Genetic Variant of C-5434T REN Enhancer on Serum Renin Levels and Binding Pattern of Signal Transducers and Activators Transcription 3Imama Maslahah0Mohammad Saifur Rohman1Nashi Widodo2Agustina Tri Endharti3Didik Utomo4Biomedical Sciences, Faculty of Medicine, University of Brawijaya, Malang 65145, IndonesiaDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, Brawijaya University, Saiful Anwar General Hospital, Malang 65145, IndonesiaBiology Department, Faculty of Mathematics and Sciences, University of Brawijaya, Malang 65145, IndonesiaParasitology Department, Faculty of Medicine, University of Brawijaya, Malang 65145, IndonesiaBiology Department, Faculty of Mathematics and Sciences, University of Brawijaya, Malang 65145, IndonesiaThe human renin gene has been widely known to be involved in essential hypertension (EH) pathogenesis. Genetic variant C-5434T of REN enhancer contributed to renin gene transcription and serum renin regulation. However, the mechanism associated with the transcription level changes remains unknown, and only a few reports exist that discussed serum renin levels on C-5434T of REN. Thus, this study aims to investigate the relationship between genetic variant C-5434T of REN enhancer and serum renin levels in Indonesian hypertensive patients. SNP of C-5434T was genotyped in 56 hypertensive patients by using RFLP. The data showed that serum renin is slightly higher in hypertensive patients with the TT genotype (39 ± 10.3) than patients with the CC genotype (33 ± 10.6) but the difference was not statistically significant (p=0.35). Here, we also present a docking approach for predicting interaction between genetic variant -5434C/T and STAT3 (Signal Transducers and Activators Transcription 3), the predicted transcription factor that regulates renin gene enhancer. The results showed that STAT3-DNA allele T more favorably binds to DNA than STAT3-DNA allele C. These data suggest that the presence of genetic variant C-5434T has changed the binding pattern of STAT3 to REN enhancer. This is likely to influence STAT3 activity to stimulate the expression of renin gene in producing renin.http://dx.doi.org/10.1155/2015/486961 |
| spellingShingle | Imama Maslahah Mohammad Saifur Rohman Nashi Widodo Agustina Tri Endharti Didik Utomo Genetic Variant of C-5434T REN Enhancer on Serum Renin Levels and Binding Pattern of Signal Transducers and Activators Transcription 3 International Journal of Hypertension |
| title | Genetic Variant of C-5434T REN Enhancer on Serum Renin Levels and Binding Pattern of Signal Transducers and Activators Transcription 3 |
| title_full | Genetic Variant of C-5434T REN Enhancer on Serum Renin Levels and Binding Pattern of Signal Transducers and Activators Transcription 3 |
| title_fullStr | Genetic Variant of C-5434T REN Enhancer on Serum Renin Levels and Binding Pattern of Signal Transducers and Activators Transcription 3 |
| title_full_unstemmed | Genetic Variant of C-5434T REN Enhancer on Serum Renin Levels and Binding Pattern of Signal Transducers and Activators Transcription 3 |
| title_short | Genetic Variant of C-5434T REN Enhancer on Serum Renin Levels and Binding Pattern of Signal Transducers and Activators Transcription 3 |
| title_sort | genetic variant of c 5434t ren enhancer on serum renin levels and binding pattern of signal transducers and activators transcription 3 |
| url | http://dx.doi.org/10.1155/2015/486961 |
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