FMT reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal Bifidobacteria and fecal bacteria
Severe acute pancreatitis (SAP) is one of the leading causes of hospital admissions for gastrointestinal diseases, with a rising incidence worldwide. Intestinal microbiota dysbiosis caused by SAP exacerbates systemic inflammatory response syndrome and organ dysfunction. Fecal microbiota transplanta...
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2024-12-01
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| Series: | Biomolecules & Biomedicine |
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| Online Access: | https://bjbms.org/ojs/index.php/bjbms/article/view/11445 |
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| author | Yanning Mao Yandong Huang Weiwei Zhang Huiping Liang Fengming Liu Qi Luo Chunqin Xu Yi Qin Jiawen Liu Shaobo Tang Huaying Liu Xiaolong Ge |
| author_facet | Yanning Mao Yandong Huang Weiwei Zhang Huiping Liang Fengming Liu Qi Luo Chunqin Xu Yi Qin Jiawen Liu Shaobo Tang Huaying Liu Xiaolong Ge |
| author_sort | Yanning Mao |
| collection | DOAJ |
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Severe acute pancreatitis (SAP) is one of the leading causes of hospital admissions for gastrointestinal diseases, with a rising incidence worldwide. Intestinal microbiota dysbiosis caused by SAP exacerbates systemic inflammatory response syndrome and organ dysfunction. Fecal microbiota transplantation (FMT) has emerged as a promising therapeutic option for gastrointestinal diseases. In this study, fecal samples from healthy, control, and FMT-treated groups were analyzed using 16S rRNA sequencing to assess microbiome abundance and diversity. Composition and functional prediction analyses were conducted to explore the mechanisms underlying FMT in SAP. FMT significantly improved clinical parameters in SAP patients, including leukocyte count, C-reactive protein (CRP), neutrophil granulocyte count, lactate dehydrogenase (LDH), and calcitonin (P < 0.05). Organ failure rates significantly increased in the control group but decreased in the FMT group after treatment (P < 0.05). Fecal microbiota sequencing revealed that FMT significantly upregulated the abundance of Bifidobacterium longum among all SAP patients (P < 0.05). Receiver operating characteristic (ROC) curve analysis indicated that Bifidobacterium longum might play a critical role in the efficacy of FMT, with an area under the curve (AUC) value of 0.84. Additionally, there was a negative correlation between Bifidobacterium longum abundance and procalcitonin (PCT) levels, as well as a negative correlation between Escherichia coli abundance and both CT and Ca values (P < 0.05). The relative abundances of Bifidobacterium longum and Escherichia coli were significantly higher in the FMT group compared to the Bifidobacterium triple viable group (P < 0.05). In conclusion, this research supports FMT as a safe and effective intervention for treating SAP patients.
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| format | Article |
| id | doaj-art-f44d522d354948ad8aa7c5105af16524 |
| institution | Kabale University |
| issn | 2831-0896 2831-090X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj-art-f44d522d354948ad8aa7c5105af165242025-02-03T16:42:11ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2024-12-0110.17305/bb.2024.11445FMT reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal Bifidobacteria and fecal bacteriaYanning Mao0Yandong Huang1Weiwei Zhang2Huiping Liang3Fengming Liu4Qi Luo5Chunqin Xu6 Yi Qin7 Jiawen Liu8Shaobo Tang9Huaying Liu10https://orcid.org/0000-0003-0230-537XXiaolong Ge11Department of Gastroenterology, The First People's Hospital of Nanning, Nanning, ChinaDepartment of Gastroenterology, The First People's Hospital of Nanning, Nanning, ChinaDepartment of Gastroenterology, The First People's Hospital of Nanning, Nanning, ChinaDepartment of Medicine, GuangXi Health Science College, Nanning, ChinaCritical Care Medicine, The First People's Hospital of Nanning, Nanning, ChinaDepartment of Gastroenterology, The First People's Hospital of Nanning, Nanning, ChinaDepartment of Gastroenterology, The First People's Hospital of Nanning, Nanning, ChinaDepartment of Gastroenterology, The First People's Hospital of Nanning, Nanning, ChinaDepartment of Gastroenterology, The First People's Hospital of Nanning, Nanning, ChinaDepartment of Gastroenterology, The First People's Hospital of Nanning, Nanning, ChinaDepartment of Medicine, GuangXi Health Science College, Nanning, ChinaDepartment of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China Severe acute pancreatitis (SAP) is one of the leading causes of hospital admissions for gastrointestinal diseases, with a rising incidence worldwide. Intestinal microbiota dysbiosis caused by SAP exacerbates systemic inflammatory response syndrome and organ dysfunction. Fecal microbiota transplantation (FMT) has emerged as a promising therapeutic option for gastrointestinal diseases. In this study, fecal samples from healthy, control, and FMT-treated groups were analyzed using 16S rRNA sequencing to assess microbiome abundance and diversity. Composition and functional prediction analyses were conducted to explore the mechanisms underlying FMT in SAP. FMT significantly improved clinical parameters in SAP patients, including leukocyte count, C-reactive protein (CRP), neutrophil granulocyte count, lactate dehydrogenase (LDH), and calcitonin (P < 0.05). Organ failure rates significantly increased in the control group but decreased in the FMT group after treatment (P < 0.05). Fecal microbiota sequencing revealed that FMT significantly upregulated the abundance of Bifidobacterium longum among all SAP patients (P < 0.05). Receiver operating characteristic (ROC) curve analysis indicated that Bifidobacterium longum might play a critical role in the efficacy of FMT, with an area under the curve (AUC) value of 0.84. Additionally, there was a negative correlation between Bifidobacterium longum abundance and procalcitonin (PCT) levels, as well as a negative correlation between Escherichia coli abundance and both CT and Ca values (P < 0.05). The relative abundances of Bifidobacterium longum and Escherichia coli were significantly higher in the FMT group compared to the Bifidobacterium triple viable group (P < 0.05). In conclusion, this research supports FMT as a safe and effective intervention for treating SAP patients. https://bjbms.org/ojs/index.php/bjbms/article/view/11445Severe acute pancreatitisSAPfecal microbial transplantationFMTclinical efficacy |
| spellingShingle | Yanning Mao Yandong Huang Weiwei Zhang Huiping Liang Fengming Liu Qi Luo Chunqin Xu Yi Qin Jiawen Liu Shaobo Tang Huaying Liu Xiaolong Ge FMT reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal Bifidobacteria and fecal bacteria Biomolecules & Biomedicine Severe acute pancreatitis SAP fecal microbial transplantation FMT clinical efficacy |
| title | FMT reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal Bifidobacteria and fecal bacteria |
| title_full | FMT reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal Bifidobacteria and fecal bacteria |
| title_fullStr | FMT reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal Bifidobacteria and fecal bacteria |
| title_full_unstemmed | FMT reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal Bifidobacteria and fecal bacteria |
| title_short | FMT reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal Bifidobacteria and fecal bacteria |
| title_sort | fmt reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal bifidobacteria and fecal bacteria |
| topic | Severe acute pancreatitis SAP fecal microbial transplantation FMT clinical efficacy |
| url | https://bjbms.org/ojs/index.php/bjbms/article/view/11445 |
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