Flaxseed Ingestion Alters Ratio of Enterolactone Enantiomers in Human Serum
Enterolactone (EL) is an enterolignan found in human subjects. In this pilot study, the enantiomeric ratios of serum EL were determined in serum from healthy adults during consumption of habitual diet, and after an 8-day supplementation with flaxseed (25 g/day). (−)EL dominated in all serum samples...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2010/403076 |
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| author | Niina M. Saarinen Annika I. Smeds José L. Peñalvo Tarja Nurmi Herman Adlercreutz Sari Mäkelä |
| author_facet | Niina M. Saarinen Annika I. Smeds José L. Peñalvo Tarja Nurmi Herman Adlercreutz Sari Mäkelä |
| author_sort | Niina M. Saarinen |
| collection | DOAJ |
| description | Enterolactone (EL) is an enterolignan found in human subjects. In this pilot study, the enantiomeric ratios of serum EL were determined in serum from healthy adults during consumption of habitual diet, and after an 8-day supplementation with flaxseed (25 g/day). (−)EL dominated in all serum samples collected during habitual diet consumption. However, the ratio of (−)EL and (+)EL enantiomers differed markedly between individuals. Flaxseed ingestion increased significantly the proportion of (+)EL in all subjects. Moreover, a small but significant increase in serum (−)EL concentration was measured. After flaxseed ingestion, (−)EL concentrations correlated with those of (+)EL suggesting that the stereochemistry of the parent plant lignan in flaxseed is not a major determinant of EL formation in human subjects. Comparison of EL concentrations obtained with the validated chromatographic methods (HPLC-MS/MS, HPLC-CEAD, and GC-MS) and the time-resolved fluoroimmunoassay (TR-FIA) revealed that the immunoassay method underestimates human serum EL concentrations after the flaxseed ingestion. |
| format | Article |
| id | doaj-art-f4437171031b4264bb834c24f43f6f1c |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-f4437171031b4264bb834c24f43f6f1c2025-02-03T01:32:32ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322010-01-01201010.1155/2010/403076403076Flaxseed Ingestion Alters Ratio of Enterolactone Enantiomers in Human SerumNiina M. Saarinen0Annika I. Smeds1José L. Peñalvo2Tarja Nurmi3Herman Adlercreutz4Sari Mäkelä5Functional Foods Forum, University of Turku, Itäinen Pitkäkatu 4A, 20014, FinlandLaboratory of Organic Chemistry, Åbo Akademi University, Biskopsgatan 8, 20500 Turku, FinlandInstitute for Preventive Medicine, Nutrition and Cancer, Folkhälsan Research Center, and Division of Clinical Chemistry, University of Helsinki, PB 63, 00014, Helsinki, FinlandResearch Institute of Public Health, School of Public Health and Clinical Nutrition, University of Kuopio, P.O. Box 1627, 70211 Kuopio, FinlandInstitute for Preventive Medicine, Nutrition and Cancer, Folkhälsan Research Center, and Division of Clinical Chemistry, University of Helsinki, PB 63, 00014, Helsinki, FinlandFunctional Foods Forum, University of Turku, Itäinen Pitkäkatu 4A, 20014, FinlandEnterolactone (EL) is an enterolignan found in human subjects. In this pilot study, the enantiomeric ratios of serum EL were determined in serum from healthy adults during consumption of habitual diet, and after an 8-day supplementation with flaxseed (25 g/day). (−)EL dominated in all serum samples collected during habitual diet consumption. However, the ratio of (−)EL and (+)EL enantiomers differed markedly between individuals. Flaxseed ingestion increased significantly the proportion of (+)EL in all subjects. Moreover, a small but significant increase in serum (−)EL concentration was measured. After flaxseed ingestion, (−)EL concentrations correlated with those of (+)EL suggesting that the stereochemistry of the parent plant lignan in flaxseed is not a major determinant of EL formation in human subjects. Comparison of EL concentrations obtained with the validated chromatographic methods (HPLC-MS/MS, HPLC-CEAD, and GC-MS) and the time-resolved fluoroimmunoassay (TR-FIA) revealed that the immunoassay method underestimates human serum EL concentrations after the flaxseed ingestion.http://dx.doi.org/10.1155/2010/403076 |
| spellingShingle | Niina M. Saarinen Annika I. Smeds José L. Peñalvo Tarja Nurmi Herman Adlercreutz Sari Mäkelä Flaxseed Ingestion Alters Ratio of Enterolactone Enantiomers in Human Serum Journal of Nutrition and Metabolism |
| title | Flaxseed Ingestion Alters Ratio of Enterolactone Enantiomers in Human Serum |
| title_full | Flaxseed Ingestion Alters Ratio of Enterolactone Enantiomers in Human Serum |
| title_fullStr | Flaxseed Ingestion Alters Ratio of Enterolactone Enantiomers in Human Serum |
| title_full_unstemmed | Flaxseed Ingestion Alters Ratio of Enterolactone Enantiomers in Human Serum |
| title_short | Flaxseed Ingestion Alters Ratio of Enterolactone Enantiomers in Human Serum |
| title_sort | flaxseed ingestion alters ratio of enterolactone enantiomers in human serum |
| url | http://dx.doi.org/10.1155/2010/403076 |
| work_keys_str_mv | AT niinamsaarinen flaxseedingestionaltersratioofenterolactoneenantiomersinhumanserum AT annikaismeds flaxseedingestionaltersratioofenterolactoneenantiomersinhumanserum AT joselpenalvo flaxseedingestionaltersratioofenterolactoneenantiomersinhumanserum AT tarjanurmi flaxseedingestionaltersratioofenterolactoneenantiomersinhumanserum AT hermanadlercreutz flaxseedingestionaltersratioofenterolactoneenantiomersinhumanserum AT sarimakela flaxseedingestionaltersratioofenterolactoneenantiomersinhumanserum |