Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays
This study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2013/420601 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832555160316936192 |
|---|---|
| author | Namrita Lall Cynthia Joan Henley-Smith Marco Nuno De Canha Carel Basson Oosthuizen Danielle Berrington |
| author_facet | Namrita Lall Cynthia Joan Henley-Smith Marco Nuno De Canha Carel Basson Oosthuizen Danielle Berrington |
| author_sort | Namrita Lall |
| collection | DOAJ |
| description | This study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and Mycobacterium tuberculosis. PrestoBlue and Alamar Blue are resazurin based reagents that resulted in a quick and easily distinguishable colour change that allowed for visual readings. INT and Sodium 3′-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate (XTT) are tetrazolium based reagents which are converted to a formazan dye in the presence of metabolically active mitochondria enzyme. For cell viability analysis, reagents XTT and PrestoBlue were compared. PrestoBlue was able to clearly indicate the minimum inhibitory concentration (MIC) of various positive drug controls on various microbial strains. PrestoBlue was also a good indicator of the 50% inhibitory concentration (IC50) of positive drug controls on various cell lines. |
| format | Article |
| id | doaj-art-f43d368b86984a239a68f73654dfe24b |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-f43d368b86984a239a68f73654dfe24b2025-02-03T05:49:28ZengWileyInternational Journal of Microbiology1687-918X1687-91982013-01-01201310.1155/2013/420601420601Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial AssaysNamrita Lall0Cynthia Joan Henley-Smith1Marco Nuno De Canha2Carel Basson Oosthuizen3Danielle Berrington4Department of Plant Science, University of Pretoria, Pretoria 0002, South AfricaDepartment of Plant Science, University of Pretoria, Pretoria 0002, South AfricaDepartment of Plant Science, University of Pretoria, Pretoria 0002, South AfricaDepartment of Plant Science, University of Pretoria, Pretoria 0002, South AfricaDepartment of Plant Science, University of Pretoria, Pretoria 0002, South AfricaThis study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and Mycobacterium tuberculosis. PrestoBlue and Alamar Blue are resazurin based reagents that resulted in a quick and easily distinguishable colour change that allowed for visual readings. INT and Sodium 3′-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate (XTT) are tetrazolium based reagents which are converted to a formazan dye in the presence of metabolically active mitochondria enzyme. For cell viability analysis, reagents XTT and PrestoBlue were compared. PrestoBlue was able to clearly indicate the minimum inhibitory concentration (MIC) of various positive drug controls on various microbial strains. PrestoBlue was also a good indicator of the 50% inhibitory concentration (IC50) of positive drug controls on various cell lines.http://dx.doi.org/10.1155/2013/420601 |
| spellingShingle | Namrita Lall Cynthia Joan Henley-Smith Marco Nuno De Canha Carel Basson Oosthuizen Danielle Berrington Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays International Journal of Microbiology |
| title | Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays |
| title_full | Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays |
| title_fullStr | Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays |
| title_full_unstemmed | Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays |
| title_short | Viability Reagent, PrestoBlue, in Comparison with Other Available Reagents, Utilized in Cytotoxicity and Antimicrobial Assays |
| title_sort | viability reagent prestoblue in comparison with other available reagents utilized in cytotoxicity and antimicrobial assays |
| url | http://dx.doi.org/10.1155/2013/420601 |
| work_keys_str_mv | AT namritalall viabilityreagentprestoblueincomparisonwithotheravailablereagentsutilizedincytotoxicityandantimicrobialassays AT cynthiajoanhenleysmith viabilityreagentprestoblueincomparisonwithotheravailablereagentsutilizedincytotoxicityandantimicrobialassays AT marconunodecanha viabilityreagentprestoblueincomparisonwithotheravailablereagentsutilizedincytotoxicityandantimicrobialassays AT carelbassonoosthuizen viabilityreagentprestoblueincomparisonwithotheravailablereagentsutilizedincytotoxicityandantimicrobialassays AT danielleberrington viabilityreagentprestoblueincomparisonwithotheravailablereagentsutilizedincytotoxicityandantimicrobialassays |