Effectual Anticancer Potentiality of Loaded Bee Venom onto Fungal Chitosan Nanoparticles
Chitosan and its nanoparticles (NPs) could be extracted from numerous fungal species and used as effectual carriers for bioactive compounds. The fungal chitosan (FC) was innovatively acquired from Fusarium oxysporum grown mycelia, characterized and used for NP synthesis and loading with bee venom (B...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2020/2785304 |
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| author | Adel I. Alalawy Haddad A. El Rabey Fahad M. Almutairi Ahmed A. Tayel Mohammed A. Al-Duais Nahla S. Zidan Mohamed I. Sakran |
| author_facet | Adel I. Alalawy Haddad A. El Rabey Fahad M. Almutairi Ahmed A. Tayel Mohammed A. Al-Duais Nahla S. Zidan Mohamed I. Sakran |
| author_sort | Adel I. Alalawy |
| collection | DOAJ |
| description | Chitosan and its nanoparticles (NPs) could be extracted from numerous fungal species and used as effectual carriers for bioactive compounds. The fungal chitosan (FC) was innovatively acquired from Fusarium oxysporum grown mycelia, characterized and used for NP synthesis and loading with bee venom (BV). The nano-FC (NFC) had 192.4 nm mean NP diameter, 38.22% loading capacity, and 92.42% entrapment efficiency. BV release from NFC was pH and time dependent; burst BV release was detected at the first 6 h, followed by gradual releases up to 30 h. The in vitro anticancer potentiality valuation, of NFC, BV, and NFC/BV nanoconjugates against HeLa cervix carcinoma, revealed that they all had potent dose-dependent anticancer activity; BV/NFC nanoconjugates were the most effective with IC50=200 μg/mL. The fluorescent staining of treated HeLa cells with BV/NFC nanoconjugates, with DAPI and acridine orange/propidium iodide combination, indicated the appearance of early apoptosis, secondary apoptosis, and secondary necrosis markers and their increment with exposure prolongation. The production of NFC from F. oxysporum and their loading with BV are strongly counseled for production of potent natural antitumor agent with augmented activity against cervix carcinoma. |
| format | Article |
| id | doaj-art-f42e4e643f534cd998a1981ae569ec8d |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-f42e4e643f534cd998a1981ae569ec8d2025-08-20T03:25:22ZengWileyInternational Journal of Polymer Science1687-94221687-94302020-01-01202010.1155/2020/27853042785304Effectual Anticancer Potentiality of Loaded Bee Venom onto Fungal Chitosan NanoparticlesAdel I. Alalawy0Haddad A. El Rabey1Fahad M. Almutairi2Ahmed A. Tayel3Mohammed A. Al-Duais4Nahla S. Zidan5Mohamed I. Sakran6Biochemistry Department, Faculty of Science, University of Tabuk, Saudi ArabiaBiochemistry Department, Faculty of Science, University of Tabuk, Saudi ArabiaBiochemistry Department, Faculty of Science, University of Tabuk, Saudi ArabiaFaculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, EgyptBiochemistry Department, Faculty of Science, University of Tabuk, Saudi ArabiaDepartment of Nutrition and Food Science, Faculty of Home Economics, University of Tabuk, Saudi ArabiaBiochemistry Department, Faculty of Science, University of Tabuk, Saudi ArabiaChitosan and its nanoparticles (NPs) could be extracted from numerous fungal species and used as effectual carriers for bioactive compounds. The fungal chitosan (FC) was innovatively acquired from Fusarium oxysporum grown mycelia, characterized and used for NP synthesis and loading with bee venom (BV). The nano-FC (NFC) had 192.4 nm mean NP diameter, 38.22% loading capacity, and 92.42% entrapment efficiency. BV release from NFC was pH and time dependent; burst BV release was detected at the first 6 h, followed by gradual releases up to 30 h. The in vitro anticancer potentiality valuation, of NFC, BV, and NFC/BV nanoconjugates against HeLa cervix carcinoma, revealed that they all had potent dose-dependent anticancer activity; BV/NFC nanoconjugates were the most effective with IC50=200 μg/mL. The fluorescent staining of treated HeLa cells with BV/NFC nanoconjugates, with DAPI and acridine orange/propidium iodide combination, indicated the appearance of early apoptosis, secondary apoptosis, and secondary necrosis markers and their increment with exposure prolongation. The production of NFC from F. oxysporum and their loading with BV are strongly counseled for production of potent natural antitumor agent with augmented activity against cervix carcinoma.http://dx.doi.org/10.1155/2020/2785304 |
| spellingShingle | Adel I. Alalawy Haddad A. El Rabey Fahad M. Almutairi Ahmed A. Tayel Mohammed A. Al-Duais Nahla S. Zidan Mohamed I. Sakran Effectual Anticancer Potentiality of Loaded Bee Venom onto Fungal Chitosan Nanoparticles International Journal of Polymer Science |
| title | Effectual Anticancer Potentiality of Loaded Bee Venom onto Fungal Chitosan Nanoparticles |
| title_full | Effectual Anticancer Potentiality of Loaded Bee Venom onto Fungal Chitosan Nanoparticles |
| title_fullStr | Effectual Anticancer Potentiality of Loaded Bee Venom onto Fungal Chitosan Nanoparticles |
| title_full_unstemmed | Effectual Anticancer Potentiality of Loaded Bee Venom onto Fungal Chitosan Nanoparticles |
| title_short | Effectual Anticancer Potentiality of Loaded Bee Venom onto Fungal Chitosan Nanoparticles |
| title_sort | effectual anticancer potentiality of loaded bee venom onto fungal chitosan nanoparticles |
| url | http://dx.doi.org/10.1155/2020/2785304 |
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