The Function of Two <i>Brassica napus</i> β-Ketoacyl-CoA Synthases on the Fatty Acid Composition
Rapeseed (<i>Brassica napus</i> L.) is one of the four major oilseed crops in the world and is rich in fatty acids. Changes in the fatty acid composition affect the quality of rapeseed. Fatty acids play various roles in plants, but the functions of the genes involved in the fatty acid co...
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2025-01-01
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| author | Dongfang Zhao Bingqian Zhou Bo Hong Jiajun Mao Hu Chen Junjie Wu Li Liao Chunyun Guan Mei Guan |
| author_facet | Dongfang Zhao Bingqian Zhou Bo Hong Jiajun Mao Hu Chen Junjie Wu Li Liao Chunyun Guan Mei Guan |
| author_sort | Dongfang Zhao |
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| description | Rapeseed (<i>Brassica napus</i> L.) is one of the four major oilseed crops in the world and is rich in fatty acids. Changes in the fatty acid composition affect the quality of rapeseed. Fatty acids play various roles in plants, but the functions of the genes involved in the fatty acid composition during plant development remain unclear. β-Ketoacyl-CoA synthase (KCS) is a key enzyme involved in the elongation of fatty acids. Various types of fatty acid products are used to build lipid molecules, such as oils, suberin, wax, and membrane lipids. In <i>B. napus</i>, <i>BnaKCSA8</i> and <i>BnaKCSC3</i> belong to the KCS family, but their specific functions remain unclear. This study cloned <i>BnaKCSA8</i> and <i>BnaKCSC3</i> from <i>Brassica napus</i> L. and analyzed their functions. The gene structures of <i>BnaKCSA8</i> and <i>BnaKCSC3</i> were similar and they were localized to the endoplasmic reticulum (ER). In yeast, overexpression of <i>BnaKCSA8</i> increased the ratios of palmitoleic acid and oleic acid, while <i>BnaKCSC3</i> decreased the ratios of oleic acid. In Arabidopsis, overexpression of <i>BnaKCSA8</i> and <i>BnaKCSC3</i> lead to an increase in the proportion of linoleic acid and a decrease in the erucic acid. In summary, <i>BnaKCSA8</i> and <i>BnaKCSC3</i> altered the composition ratios of fatty acids. These findings lay the foundation for an understanding of the role of KCS in the fatty acids in rapeseed, potentially improving its health and nutritional qualities. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-f42b1c33a6a04a1f810825d02be7d8242025-01-24T13:46:44ZengMDPI AGPlants2223-77472025-01-0114220210.3390/plants14020202The Function of Two <i>Brassica napus</i> β-Ketoacyl-CoA Synthases on the Fatty Acid CompositionDongfang Zhao0Bingqian Zhou1Bo Hong2Jiajun Mao3Hu Chen4Junjie Wu5Li Liao6Chunyun Guan7Mei Guan8College of Agriculture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agriculture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agriculture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agriculture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agriculture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agriculture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agriculture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agriculture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Agriculture, Hunan Agricultural University, Changsha 410128, ChinaRapeseed (<i>Brassica napus</i> L.) is one of the four major oilseed crops in the world and is rich in fatty acids. Changes in the fatty acid composition affect the quality of rapeseed. Fatty acids play various roles in plants, but the functions of the genes involved in the fatty acid composition during plant development remain unclear. β-Ketoacyl-CoA synthase (KCS) is a key enzyme involved in the elongation of fatty acids. Various types of fatty acid products are used to build lipid molecules, such as oils, suberin, wax, and membrane lipids. In <i>B. napus</i>, <i>BnaKCSA8</i> and <i>BnaKCSC3</i> belong to the KCS family, but their specific functions remain unclear. This study cloned <i>BnaKCSA8</i> and <i>BnaKCSC3</i> from <i>Brassica napus</i> L. and analyzed their functions. The gene structures of <i>BnaKCSA8</i> and <i>BnaKCSC3</i> were similar and they were localized to the endoplasmic reticulum (ER). In yeast, overexpression of <i>BnaKCSA8</i> increased the ratios of palmitoleic acid and oleic acid, while <i>BnaKCSC3</i> decreased the ratios of oleic acid. In Arabidopsis, overexpression of <i>BnaKCSA8</i> and <i>BnaKCSC3</i> lead to an increase in the proportion of linoleic acid and a decrease in the erucic acid. In summary, <i>BnaKCSA8</i> and <i>BnaKCSC3</i> altered the composition ratios of fatty acids. These findings lay the foundation for an understanding of the role of KCS in the fatty acids in rapeseed, potentially improving its health and nutritional qualities.https://www.mdpi.com/2223-7747/14/2/202<i>Brassica napus</i> L.β-ketoacyl-CoA synthase (KCS)fatty acid composition |
| spellingShingle | Dongfang Zhao Bingqian Zhou Bo Hong Jiajun Mao Hu Chen Junjie Wu Li Liao Chunyun Guan Mei Guan The Function of Two <i>Brassica napus</i> β-Ketoacyl-CoA Synthases on the Fatty Acid Composition Plants <i>Brassica napus</i> L. β-ketoacyl-CoA synthase (KCS) fatty acid composition |
| title | The Function of Two <i>Brassica napus</i> β-Ketoacyl-CoA Synthases on the Fatty Acid Composition |
| title_full | The Function of Two <i>Brassica napus</i> β-Ketoacyl-CoA Synthases on the Fatty Acid Composition |
| title_fullStr | The Function of Two <i>Brassica napus</i> β-Ketoacyl-CoA Synthases on the Fatty Acid Composition |
| title_full_unstemmed | The Function of Two <i>Brassica napus</i> β-Ketoacyl-CoA Synthases on the Fatty Acid Composition |
| title_short | The Function of Two <i>Brassica napus</i> β-Ketoacyl-CoA Synthases on the Fatty Acid Composition |
| title_sort | function of two i brassica napus i β ketoacyl coa synthases on the fatty acid composition |
| topic | <i>Brassica napus</i> L. β-ketoacyl-CoA synthase (KCS) fatty acid composition |
| url | https://www.mdpi.com/2223-7747/14/2/202 |
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