Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats
Objectives. To observe the therapeutic effects of Acanthopanax and 3-methyladenine against severe acute pancreatitis (SAP). Methods. Sodium taurocholate-induced SAP rats were equally randomized into a SAP group, an Acanthopanax group, and a 3-methyladenine group. Serum amylase levels were determined...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/8369704 |
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| author | Xiaohong Wang Guoxiong Zhou Chun Liu Ronglong Wei Shunxing Zhu Yuefen Xu Mengjie Wu Qing Miao |
| author_facet | Xiaohong Wang Guoxiong Zhou Chun Liu Ronglong Wei Shunxing Zhu Yuefen Xu Mengjie Wu Qing Miao |
| author_sort | Xiaohong Wang |
| collection | DOAJ |
| description | Objectives. To observe the therapeutic effects of Acanthopanax and 3-methyladenine against severe acute pancreatitis (SAP). Methods. Sodium taurocholate-induced SAP rats were equally randomized into a SAP group, an Acanthopanax group, and a 3-methyladenine group. Serum amylase levels were determined by ELISA; protein and mRNA expression levels of nucleus nuclear factor kappa B (NF-κB) p65, light chain 3II (LC3-II), and Beclin-1 and mRNA expression levels of Class III phosphatidylinositol 3-kinase (PI3K-III) in pancreas tissue were detected by Western blot and quantitative real-time PCR, respectively; mortality and pathological change of the pancreas were observed at 3, 12, and 24 h after operation. Results. There was no significant difference in mortality between SAP group and both treatment groups (P>0.05). Serum amylase levels, protein, and mRNA expression levels of nucleus NF-κB p65, LC3-II, and Beclin-1 protein, mRNA expression levels of PI3K-III, and pathological score of the pancreas in both treatment groups were significantly lower than those in SAP group at 12 and 24 h after operation (P<0.05 or 0.01). The number of autophagosomes and autophagolysosomes of pancreatic acinar cells in both treatment groups was smaller than that in SAP group at 12 and 24 h. Conclusions. Acanthopanax and 3-methyladenine had similar therapeutic effects against SAP in rats. The mechanism may be through inhibiting abnormal autophagy activation of pancreatic acinar cells. |
| format | Article |
| id | doaj-art-f42146474b564c1da39cd66ac64e5ff8 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-f42146474b564c1da39cd66ac64e5ff82025-02-03T06:11:38ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/83697048369704Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in RatsXiaohong Wang0Guoxiong Zhou1Chun Liu2Ronglong Wei3Shunxing Zhu4Yuefen Xu5Mengjie Wu6Qing Miao7Department of Gastroenterology, Danyang People’s Hospital, Danyang 212300, ChinaDepartment of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, ChinaLaboratory Animal Center of Nantong University, Nantong 226001, ChinaDepartment of Gastroenterology, Danyang People’s Hospital, Danyang 212300, ChinaLaboratory Animal Center of Nantong University, Nantong 226001, ChinaDepartment of Gastroenterology, Danyang People’s Hospital, Danyang 212300, ChinaDepartment of Gastroenterology, Danyang People’s Hospital, Danyang 212300, ChinaDepartment of Gastroenterology, Danyang People’s Hospital, Danyang 212300, ChinaObjectives. To observe the therapeutic effects of Acanthopanax and 3-methyladenine against severe acute pancreatitis (SAP). Methods. Sodium taurocholate-induced SAP rats were equally randomized into a SAP group, an Acanthopanax group, and a 3-methyladenine group. Serum amylase levels were determined by ELISA; protein and mRNA expression levels of nucleus nuclear factor kappa B (NF-κB) p65, light chain 3II (LC3-II), and Beclin-1 and mRNA expression levels of Class III phosphatidylinositol 3-kinase (PI3K-III) in pancreas tissue were detected by Western blot and quantitative real-time PCR, respectively; mortality and pathological change of the pancreas were observed at 3, 12, and 24 h after operation. Results. There was no significant difference in mortality between SAP group and both treatment groups (P>0.05). Serum amylase levels, protein, and mRNA expression levels of nucleus NF-κB p65, LC3-II, and Beclin-1 protein, mRNA expression levels of PI3K-III, and pathological score of the pancreas in both treatment groups were significantly lower than those in SAP group at 12 and 24 h after operation (P<0.05 or 0.01). The number of autophagosomes and autophagolysosomes of pancreatic acinar cells in both treatment groups was smaller than that in SAP group at 12 and 24 h. Conclusions. Acanthopanax and 3-methyladenine had similar therapeutic effects against SAP in rats. The mechanism may be through inhibiting abnormal autophagy activation of pancreatic acinar cells.http://dx.doi.org/10.1155/2016/8369704 |
| spellingShingle | Xiaohong Wang Guoxiong Zhou Chun Liu Ronglong Wei Shunxing Zhu Yuefen Xu Mengjie Wu Qing Miao Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats Mediators of Inflammation |
| title | Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats |
| title_full | Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats |
| title_fullStr | Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats |
| title_full_unstemmed | Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats |
| title_short | Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats |
| title_sort | acanthopanax versus 3 methyladenine ameliorates sodium taurocholate induced severe acute pancreatitis by inhibiting the autophagic pathway in rats |
| url | http://dx.doi.org/10.1155/2016/8369704 |
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