Characterization of <i>Clostridium perfringens</i> Phage Endolysin PlyDolk21
<b>Background:</b> <i>Clostridium perfringens</i> is a significant cause of food poisoning. Broad-spectrum antibiotics, commonly used to control <i>C. perfringens</i>, are becoming less effective due to the rise of antibiotic-resistant strains, necessitating alter...
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MDPI AG
2025-01-01
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| author | Suyoung Seo Bokyung Son Minsuk Kong |
| author_facet | Suyoung Seo Bokyung Son Minsuk Kong |
| author_sort | Suyoung Seo |
| collection | DOAJ |
| description | <b>Background:</b> <i>Clostridium perfringens</i> is a significant cause of food poisoning. Broad-spectrum antibiotics, commonly used to control <i>C. perfringens</i>, are becoming less effective due to the rise of antibiotic-resistant strains, necessitating alternative control strategies. <b>Methods:</b> A <i>C. perfringens</i>-infecting bacteriophage, Dolk21, and its endolysin, PlyDolk21, were isolated and characterized. The lytic activity of PlyDolk21 was assessed in comparison to its catalytic domain alone. Both PlyDolk21 and its cell wall binding domain (CBD) were evaluated in beef and milk for their antimicrobial activity and cell wall binding activity, respectively. <b>Results:</b> While phage Dolk21 was specific to certain <i>C. perfringens</i> strains, PlyDolk21 exhibited lytic activity against all <i>C. perfringens</i> strains tested. The full-length PlyDolk21 showed stronger lytic activity compared to its catalytic domain alone. PlyDolk21_CBD successfully bound to <i>C. perfringens</i> in vitro and in foods. Additionally, PlyDolk21 effectively reduced the viable cell counts of <i>C. perfringens</i> by 3-log in beef soup and milk samples. <b>Conclusions:</b> This study demonstrates that PlyDolk21 and its CBD hold potential as a biocontrol and detection agent targeting <i>C. perfringens</i> in various food matrices. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-f413edff6edf4e8d9069a5743d08b7052025-01-24T13:18:54ZengMDPI AGAntibiotics2079-63822025-01-011418110.3390/antibiotics14010081Characterization of <i>Clostridium perfringens</i> Phage Endolysin PlyDolk21Suyoung Seo0Bokyung Son1Minsuk Kong2Department of Food Science and Biotechnology, Research Institute of Food and Biotechnology, Seoul National University of Science and Technology, Seoul 01811, Republic of KoreaDepartment of Food Biotechnology, Dong-A University, Busan 49315, Republic of KoreaDepartment of Food Science and Biotechnology, Research Institute of Food and Biotechnology, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea<b>Background:</b> <i>Clostridium perfringens</i> is a significant cause of food poisoning. Broad-spectrum antibiotics, commonly used to control <i>C. perfringens</i>, are becoming less effective due to the rise of antibiotic-resistant strains, necessitating alternative control strategies. <b>Methods:</b> A <i>C. perfringens</i>-infecting bacteriophage, Dolk21, and its endolysin, PlyDolk21, were isolated and characterized. The lytic activity of PlyDolk21 was assessed in comparison to its catalytic domain alone. Both PlyDolk21 and its cell wall binding domain (CBD) were evaluated in beef and milk for their antimicrobial activity and cell wall binding activity, respectively. <b>Results:</b> While phage Dolk21 was specific to certain <i>C. perfringens</i> strains, PlyDolk21 exhibited lytic activity against all <i>C. perfringens</i> strains tested. The full-length PlyDolk21 showed stronger lytic activity compared to its catalytic domain alone. PlyDolk21_CBD successfully bound to <i>C. perfringens</i> in vitro and in foods. Additionally, PlyDolk21 effectively reduced the viable cell counts of <i>C. perfringens</i> by 3-log in beef soup and milk samples. <b>Conclusions:</b> This study demonstrates that PlyDolk21 and its CBD hold potential as a biocontrol and detection agent targeting <i>C. perfringens</i> in various food matrices.https://www.mdpi.com/2079-6382/14/1/81<i>Clostridium perfringens</i>bacteriophageendolysincell wall-binding domainbiocontrol |
| spellingShingle | Suyoung Seo Bokyung Son Minsuk Kong Characterization of <i>Clostridium perfringens</i> Phage Endolysin PlyDolk21 Antibiotics <i>Clostridium perfringens</i> bacteriophage endolysin cell wall-binding domain biocontrol |
| title | Characterization of <i>Clostridium perfringens</i> Phage Endolysin PlyDolk21 |
| title_full | Characterization of <i>Clostridium perfringens</i> Phage Endolysin PlyDolk21 |
| title_fullStr | Characterization of <i>Clostridium perfringens</i> Phage Endolysin PlyDolk21 |
| title_full_unstemmed | Characterization of <i>Clostridium perfringens</i> Phage Endolysin PlyDolk21 |
| title_short | Characterization of <i>Clostridium perfringens</i> Phage Endolysin PlyDolk21 |
| title_sort | characterization of i clostridium perfringens i phage endolysin plydolk21 |
| topic | <i>Clostridium perfringens</i> bacteriophage endolysin cell wall-binding domain biocontrol |
| url | https://www.mdpi.com/2079-6382/14/1/81 |
| work_keys_str_mv | AT suyoungseo characterizationoficlostridiumperfringensiphageendolysinplydolk21 AT bokyungson characterizationoficlostridiumperfringensiphageendolysinplydolk21 AT minsukkong characterizationoficlostridiumperfringensiphageendolysinplydolk21 |