Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages
During an infection, lipopolysaccharide (LPS) stimulates the production of reactive oxygen species (ROS), which is mediated, in large part, by nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs); NOX2 is the major NOX isoform found in the macrophage cell membrane. While the immunomod...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/325481 |
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| author | Tao Meng Jingya Yu Zhen Lei Jianbo Wu Shuqin Wang Qiyu Bo Xinyu Zhang Zhiyong Ma Jingui Yu |
| author_facet | Tao Meng Jingya Yu Zhen Lei Jianbo Wu Shuqin Wang Qiyu Bo Xinyu Zhang Zhiyong Ma Jingui Yu |
| author_sort | Tao Meng |
| collection | DOAJ |
| description | During an infection, lipopolysaccharide (LPS) stimulates the production of reactive oxygen species (ROS), which is mediated, in large part, by nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs); NOX2 is the major NOX isoform found in the macrophage cell membrane. While the immunomodulatory activity of propofol is highly documented, its effect on the LPS-induced NOX2/ROS/NF-κB signaling pathway in macrophages has not been addressed. In present study, we used murine macrophage cell line RAW264.7 pretreated with propofol and stimulated with LPS. IL-6 and TNF-α expression, ROS production, and NOX activity were determined. Results showed that propofol attenuated LPS-induced TNF-α and IL-6 expression. Moreover, LPS-stimulated phosphorylation of NF-κB and generation of ROS were weakened in response to propofol. Propofol also reduced LPS-induced NOX activity and expression of gp91phox and p47phox. We conclude that propofol modulates LPS signaling in macrophages by reducing NOX-mediated production of TNF-α and IL-6. |
| format | Article |
| id | doaj-art-f40f27754d8849e4a56f3912dab8ed7f |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-f40f27754d8849e4a56f3912dab8ed7f2025-02-03T01:11:31ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/325481325481Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in MacrophagesTao Meng0Jingya Yu1Zhen Lei2Jianbo Wu3Shuqin Wang4Qiyu Bo5Xinyu Zhang6Zhiyong Ma7Jingui Yu8Department of Anesthesiology, Qilu Hospital of Shandong University, Shandong University, No. 107 Wen Hua Xi Road, Jinan, Shandong 250012, ChinaDepartment of Pathogeny Biology, Shandong University School of Medicine, No. 44 Wen Hua Xi Road, Jinan, Shandong 250012, ChinaDepartment of Anesthesiology, Qilu Hospital of Shandong University, Shandong University, No. 107 Wen Hua Xi Road, Jinan, Shandong 250012, ChinaDepartment of Anesthesiology, Qilu Hospital of Shandong University, Shandong University, No. 107 Wen Hua Xi Road, Jinan, Shandong 250012, ChinaDepartment of Anesthesiology, Qilu Hospital of Shandong University, Shandong University, No. 107 Wen Hua Xi Road, Jinan, Shandong 250012, ChinaDepartment of Anesthesiology, Qilu Hospital of Shandong University, Shandong University, No. 107 Wen Hua Xi Road, Jinan, Shandong 250012, ChinaDepartment of Cardiology, Qilu Hospital of Shandong University, Shandong University, No. 107 Wen Hua Xi Road, Jinan, Shandong 250012, ChinaDepartment of Cardiology, Qilu Hospital of Shandong University, Shandong University, No. 107 Wen Hua Xi Road, Jinan, Shandong 250012, ChinaDepartment of Anesthesiology, Qilu Hospital of Shandong University, Shandong University, No. 107 Wen Hua Xi Road, Jinan, Shandong 250012, ChinaDuring an infection, lipopolysaccharide (LPS) stimulates the production of reactive oxygen species (ROS), which is mediated, in large part, by nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs); NOX2 is the major NOX isoform found in the macrophage cell membrane. While the immunomodulatory activity of propofol is highly documented, its effect on the LPS-induced NOX2/ROS/NF-κB signaling pathway in macrophages has not been addressed. In present study, we used murine macrophage cell line RAW264.7 pretreated with propofol and stimulated with LPS. IL-6 and TNF-α expression, ROS production, and NOX activity were determined. Results showed that propofol attenuated LPS-induced TNF-α and IL-6 expression. Moreover, LPS-stimulated phosphorylation of NF-κB and generation of ROS were weakened in response to propofol. Propofol also reduced LPS-induced NOX activity and expression of gp91phox and p47phox. We conclude that propofol modulates LPS signaling in macrophages by reducing NOX-mediated production of TNF-α and IL-6.http://dx.doi.org/10.1155/2013/325481 |
| spellingShingle | Tao Meng Jingya Yu Zhen Lei Jianbo Wu Shuqin Wang Qiyu Bo Xinyu Zhang Zhiyong Ma Jingui Yu Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages Clinical and Developmental Immunology |
| title | Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages |
| title_full | Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages |
| title_fullStr | Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages |
| title_full_unstemmed | Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages |
| title_short | Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages |
| title_sort | propofol reduces lipopolysaccharide induced nadph oxidase nox2 mediated tnf α and il 6 production in macrophages |
| url | http://dx.doi.org/10.1155/2013/325481 |
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