Comparative analysis of the chemical composition and size of starch granules in grain between diploid and tetraploid sweetcorn cultivars
Background. Improving the taste and nutritional value of sweetcorn using genetic selection techniques is an important trend. The tetraploid sugary maize cultivar ‘Baksanskaya sakharnaya’ (k-23426) has a number of advantages in terms of taste and marketability over traditional diploid varieties.The p...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
N.I. Vavilov All-Russian Institute of Plant Genetic Resources
2021-07-01
|
| Series: | Труды по прикладной ботанике, генетике и селекции |
| Subjects: | |
| Online Access: | https://elpub.vir.nw.ru/jour/article/view/954 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545066562879488 |
|---|---|
| author | E. B. Khatefov V. I. Khoreva Yu. A. Kerv T. V. Shelenga V. V. Sidorova Y. N. Demurin V. G. Goldstein |
| author_facet | E. B. Khatefov V. I. Khoreva Yu. A. Kerv T. V. Shelenga V. V. Sidorova Y. N. Demurin V. G. Goldstein |
| author_sort | E. B. Khatefov |
| collection | DOAJ |
| description | Background. Improving the taste and nutritional value of sweetcorn using genetic selection techniques is an important trend. The tetraploid sugary maize cultivar ‘Baksanskaya sakharnaya’ (k-23426) has a number of advantages in terms of taste and marketability over traditional diploid varieties.The purpose of the research was to identify the distinctive biochemical characteristics that affect the taste of this cultivar in comparison with the diploid reference (cv. ‘Rannyaya Lakomka’, с-1775).Materials and methods. The differences in phenotypic traits between diploid (2n) and tetraploid (4n) maize plants were assessed. Infrared spectroscopy was used to study the difference in the biochemical composition of kernels in terms of protein, starch and oil content. Accumulation of metabolites in a dry kernel was studied using gas–liquid chromatography with mass spectrometry.Results. Comparison of the phenotypic traits in 2n and 4n sweetcorn plants showed that 4n had a better ear structure, protein and oil content. Analysis of metabolites showed that quantitative indicators of chemical components changed in kernels of the 4n sweetcorn without changing the qualitative composition. The chemical composition of the metabolites in 4n sweetcorn grain was characterized by an increase in the synthesis of most organic acids and protein amino acids, phosphoric acid, fatty acids, some monosaccharides, phenolic compounds, and phytosterols. A decrease in the content of most polyhydric alcohols, disaccharides, trisaccharides, some monosaccharides, and phenolic compounds was observed. An inhibitory effect of a su2 gene dose on the size of starch granules was found. Conclusion. The studies showed the advantage of the 4n sweetcorn over the 2n one due to its improved ear structure and biochemical composition, which positively affected the taste qualities. |
| format | Article |
| id | doaj-art-f3ea8ed982d64ee5997bce490629a836 |
| institution | Kabale University |
| issn | 2227-8834 2619-0982 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | N.I. Vavilov All-Russian Institute of Plant Genetic Resources |
| record_format | Article |
| series | Труды по прикладной ботанике, генетике и селекции |
| spelling | doaj-art-f3ea8ed982d64ee5997bce490629a8362025-02-03T08:31:14ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822021-07-011822536210.30901/2227-8834-2021-2-53-62520Comparative analysis of the chemical composition and size of starch granules in grain between diploid and tetraploid sweetcorn cultivarsE. B. Khatefov0V. I. Khoreva1Yu. A. Kerv2T. V. Shelenga3V. V. Sidorova4Y. N. Demurin5V. G. Goldstein6N.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesV.S. Pustovoit All-Russian Research Institute of Oil CropsAll-Russian Research Institute for Starch Products, branch of the V.M. Gorbatov Federal Research Center for Food Systems of the RASBackground. Improving the taste and nutritional value of sweetcorn using genetic selection techniques is an important trend. The tetraploid sugary maize cultivar ‘Baksanskaya sakharnaya’ (k-23426) has a number of advantages in terms of taste and marketability over traditional diploid varieties.The purpose of the research was to identify the distinctive biochemical characteristics that affect the taste of this cultivar in comparison with the diploid reference (cv. ‘Rannyaya Lakomka’, с-1775).Materials and methods. The differences in phenotypic traits between diploid (2n) and tetraploid (4n) maize plants were assessed. Infrared spectroscopy was used to study the difference in the biochemical composition of kernels in terms of protein, starch and oil content. Accumulation of metabolites in a dry kernel was studied using gas–liquid chromatography with mass spectrometry.Results. Comparison of the phenotypic traits in 2n and 4n sweetcorn plants showed that 4n had a better ear structure, protein and oil content. Analysis of metabolites showed that quantitative indicators of chemical components changed in kernels of the 4n sweetcorn without changing the qualitative composition. The chemical composition of the metabolites in 4n sweetcorn grain was characterized by an increase in the synthesis of most organic acids and protein amino acids, phosphoric acid, fatty acids, some monosaccharides, phenolic compounds, and phytosterols. A decrease in the content of most polyhydric alcohols, disaccharides, trisaccharides, some monosaccharides, and phenolic compounds was observed. An inhibitory effect of a su2 gene dose on the size of starch granules was found. Conclusion. The studies showed the advantage of the 4n sweetcorn over the 2n one due to its improved ear structure and biochemical composition, which positively affected the taste qualities.https://elpub.vir.nw.ru/jour/article/view/954zea mays l.su2endospermgermmarkertetraploiddiploidbiochemical compositiontotal sugarsthe vir collection |
| spellingShingle | E. B. Khatefov V. I. Khoreva Yu. A. Kerv T. V. Shelenga V. V. Sidorova Y. N. Demurin V. G. Goldstein Comparative analysis of the chemical composition and size of starch granules in grain between diploid and tetraploid sweetcorn cultivars Труды по прикладной ботанике, генетике и селекции zea mays l. su2 endosperm germ marker tetraploid diploid biochemical composition total sugars the vir collection |
| title | Comparative analysis of the chemical composition and size of starch granules in grain between diploid and tetraploid sweetcorn cultivars |
| title_full | Comparative analysis of the chemical composition and size of starch granules in grain between diploid and tetraploid sweetcorn cultivars |
| title_fullStr | Comparative analysis of the chemical composition and size of starch granules in grain between diploid and tetraploid sweetcorn cultivars |
| title_full_unstemmed | Comparative analysis of the chemical composition and size of starch granules in grain between diploid and tetraploid sweetcorn cultivars |
| title_short | Comparative analysis of the chemical composition and size of starch granules in grain between diploid and tetraploid sweetcorn cultivars |
| title_sort | comparative analysis of the chemical composition and size of starch granules in grain between diploid and tetraploid sweetcorn cultivars |
| topic | zea mays l. su2 endosperm germ marker tetraploid diploid biochemical composition total sugars the vir collection |
| url | https://elpub.vir.nw.ru/jour/article/view/954 |
| work_keys_str_mv | AT ebkhatefov comparativeanalysisofthechemicalcompositionandsizeofstarchgranulesingrainbetweendiploidandtetraploidsweetcorncultivars AT vikhoreva comparativeanalysisofthechemicalcompositionandsizeofstarchgranulesingrainbetweendiploidandtetraploidsweetcorncultivars AT yuakerv comparativeanalysisofthechemicalcompositionandsizeofstarchgranulesingrainbetweendiploidandtetraploidsweetcorncultivars AT tvshelenga comparativeanalysisofthechemicalcompositionandsizeofstarchgranulesingrainbetweendiploidandtetraploidsweetcorncultivars AT vvsidorova comparativeanalysisofthechemicalcompositionandsizeofstarchgranulesingrainbetweendiploidandtetraploidsweetcorncultivars AT yndemurin comparativeanalysisofthechemicalcompositionandsizeofstarchgranulesingrainbetweendiploidandtetraploidsweetcorncultivars AT vggoldstein comparativeanalysisofthechemicalcompositionandsizeofstarchgranulesingrainbetweendiploidandtetraploidsweetcorncultivars |