Modern approaches to artificial gene synthesis: aspects of oligonucleotide synthesis, enzymatic assembly, sequence verification and error correction
Synthetic biology is a rapidly developing field aimed at engineering of biological systems with predictable properties. Synthetic biology accumulates the achievements of modern biological sciences, programming and computational modeling as well as engineering technologies for creation of biologica...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2018-08-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/1582 |
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| author | G. Y. Shevelev D. V. Pyshnyi |
| author_facet | G. Y. Shevelev D. V. Pyshnyi |
| author_sort | G. Y. Shevelev |
| collection | DOAJ |
| description | Synthetic biology is a rapidly developing field aimed at engineering of biological systems with predictable properties. Synthetic biology accumulates the achievements of modern biological sciences, programming and computational modeling as well as engineering technologies for creation of biological objects with user-defined properties. Evolution of synthetic biology has been marked by a number of technological developments in each of the mentioned fields. Thus, significant reduction in cost of DNA sequencing has provided an easy access to large amounts of data on the genetic sequences of various organisms, and decreased the price of the DNA sequence synthesis, which, analogous to Moore’s law, resulted in an opportunity to create a lot of potential genes without the time – consuming and labor – intensive traditional methods of molecular biology. Development of system biology has allowed forming a deeper understanding of the functions and relationship of natural biological models, as well as of the computational models describing processes at the cell and system levels. Combination of these factors has created an opportunity for conscious changes of natural biological systems. In this review the modern approaches to oligonucleotide gene assembly synthesis are discussed, including such aspects as protocols for gene assembly, sequence verification, error correction and further applications of synthesized genes. |
| format | Article |
| id | doaj-art-f3cd68c62dcb4dd48c838aa0af5036e3 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-f3cd68c62dcb4dd48c838aa0af5036e32025-02-01T09:58:06ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592018-08-0122549850610.18699/VJ18.387784Modern approaches to artificial gene synthesis: aspects of oligonucleotide synthesis, enzymatic assembly, sequence verification and error correctionG. Y. Shevelev0D. V. Pyshnyi1Institute of Chemical Biology and Fundamental Medicine, SB RAS; Novosibirsk State UniversityInstitute of Chemical Biology and Fundamental Medicine, SB RAS; Novosibirsk State UniversitySynthetic biology is a rapidly developing field aimed at engineering of biological systems with predictable properties. Synthetic biology accumulates the achievements of modern biological sciences, programming and computational modeling as well as engineering technologies for creation of biological objects with user-defined properties. Evolution of synthetic biology has been marked by a number of technological developments in each of the mentioned fields. Thus, significant reduction in cost of DNA sequencing has provided an easy access to large amounts of data on the genetic sequences of various organisms, and decreased the price of the DNA sequence synthesis, which, analogous to Moore’s law, resulted in an opportunity to create a lot of potential genes without the time – consuming and labor – intensive traditional methods of molecular biology. Development of system biology has allowed forming a deeper understanding of the functions and relationship of natural biological models, as well as of the computational models describing processes at the cell and system levels. Combination of these factors has created an opportunity for conscious changes of natural biological systems. In this review the modern approaches to oligonucleotide gene assembly synthesis are discussed, including such aspects as protocols for gene assembly, sequence verification, error correction and further applications of synthesized genes.https://vavilov.elpub.ru/jour/article/view/1582oligonucleotide synthesisartificial gene synthesisenzymatic gene assemblypolymerase cycling assembly (pca)ligase cycling assembly (lca)error correctionsequence verification |
| spellingShingle | G. Y. Shevelev D. V. Pyshnyi Modern approaches to artificial gene synthesis: aspects of oligonucleotide synthesis, enzymatic assembly, sequence verification and error correction Вавиловский журнал генетики и селекции oligonucleotide synthesis artificial gene synthesis enzymatic gene assembly polymerase cycling assembly (pca) ligase cycling assembly (lca) error correction sequence verification |
| title | Modern approaches to artificial gene synthesis: aspects of oligonucleotide synthesis, enzymatic assembly, sequence verification and error correction |
| title_full | Modern approaches to artificial gene synthesis: aspects of oligonucleotide synthesis, enzymatic assembly, sequence verification and error correction |
| title_fullStr | Modern approaches to artificial gene synthesis: aspects of oligonucleotide synthesis, enzymatic assembly, sequence verification and error correction |
| title_full_unstemmed | Modern approaches to artificial gene synthesis: aspects of oligonucleotide synthesis, enzymatic assembly, sequence verification and error correction |
| title_short | Modern approaches to artificial gene synthesis: aspects of oligonucleotide synthesis, enzymatic assembly, sequence verification and error correction |
| title_sort | modern approaches to artificial gene synthesis aspects of oligonucleotide synthesis enzymatic assembly sequence verification and error correction |
| topic | oligonucleotide synthesis artificial gene synthesis enzymatic gene assembly polymerase cycling assembly (pca) ligase cycling assembly (lca) error correction sequence verification |
| url | https://vavilov.elpub.ru/jour/article/view/1582 |
| work_keys_str_mv | AT gyshevelev modernapproachestoartificialgenesynthesisaspectsofoligonucleotidesynthesisenzymaticassemblysequenceverificationanderrorcorrection AT dvpyshnyi modernapproachestoartificialgenesynthesisaspectsofoligonucleotidesynthesisenzymaticassemblysequenceverificationanderrorcorrection |