Effects of Bacterial-Fungal Coinfection on Biochemical Parameters and Organ Structure in African Catfish (Clarias gariepinus)
Five infected African catfish (605±45.98 g) from the Experimental Aquaculture Base at Trakia University exhibited abnormal behaviours, including sluggish movements, lethargy, skin haemorrhages, and fin bleeding. Samples from the lesions were cultured on Sabouraud Dextrose Agar (SDA), and the fungus...
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| Format: | Article |
| Language: | English |
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Istanbul University Press
2024-10-01
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| Series: | Aquatic Sciences and Engineering |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/4184900 |
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| author | Alexander Atanasoff Dimitrinka Zapryanova Atanas Bozhkov Çiğdem Ürkü |
| author_facet | Alexander Atanasoff Dimitrinka Zapryanova Atanas Bozhkov Çiğdem Ürkü |
| author_sort | Alexander Atanasoff |
| collection | DOAJ |
| description | Five infected African catfish (605±45.98 g) from the Experimental Aquaculture Base at Trakia University exhibited abnormal behaviours, including sluggish movements, lethargy, skin haemorrhages, and fin bleeding. Samples from the lesions were cultured on Sabouraud Dextrose Agar (SDA), and the fungus was identified as Rhizopus sp. based on colony morphology and microscopy. Bacteriological samples from the kidney, spleen, liver, and blood were cultured on Tryptic Soy Agar (TSA) and Brain Heart Infusion Agar (BHIA), revealing the presence of Yersinia ruckeri, Aeromonas hydrophila, and Pseudomonas aeruginosa based on their physiological, morphological, and biochemical traits. Significant differences (p<0.01) were observed in the serum protein profiles, including total protein, albumin, globulin, and glucose levels. Histological examination showed pathological changes in the liver, kidneys, and gills, as well as haemorrhages and polymorphonuclear leukocyte infiltration in the skin lesions, with intense muscle necrosis. These biochemical and histopathological findings are valuable for monitoring health and diagnosing fungal diseases in African catfish. |
| format | Article |
| id | doaj-art-f3bef87a84064691af60bbef66b3df10 |
| institution | Kabale University |
| issn | 2602-473X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | Aquatic Sciences and Engineering |
| spelling | doaj-art-f3bef87a84064691af60bbef66b3df102025-02-04T08:44:51ZengIstanbul University PressAquatic Sciences and Engineering2602-473X2024-10-0139424825410.26650/ASE202415422274Effects of Bacterial-Fungal Coinfection on Biochemical Parameters and Organ Structure in African Catfish (Clarias gariepinus)Alexander Atanasoff0https://orcid.org/0000-0003-3460-9589Dimitrinka Zapryanova1https://orcid.org/0000-0002-0430-1690Atanas Bozhkov2https://orcid.org/0000-0001-8371-2940Çiğdem Ürkü3https://orcid.org/0000-0003-0381-9321Trakia University, Faculty of Veterinary Medicine, Department of Animal HusbandryTrakia University, Faculty of Veterinary Medicine, Department of BiochemistryTrakia University, Faculty of Agriculture, Department of AquacultureIstanbul University, Faculty of Aquatic Science, Department of Aquaculture and Fish DiseasesFive infected African catfish (605±45.98 g) from the Experimental Aquaculture Base at Trakia University exhibited abnormal behaviours, including sluggish movements, lethargy, skin haemorrhages, and fin bleeding. Samples from the lesions were cultured on Sabouraud Dextrose Agar (SDA), and the fungus was identified as Rhizopus sp. based on colony morphology and microscopy. Bacteriological samples from the kidney, spleen, liver, and blood were cultured on Tryptic Soy Agar (TSA) and Brain Heart Infusion Agar (BHIA), revealing the presence of Yersinia ruckeri, Aeromonas hydrophila, and Pseudomonas aeruginosa based on their physiological, morphological, and biochemical traits. Significant differences (p<0.01) were observed in the serum protein profiles, including total protein, albumin, globulin, and glucose levels. Histological examination showed pathological changes in the liver, kidneys, and gills, as well as haemorrhages and polymorphonuclear leukocyte infiltration in the skin lesions, with intense muscle necrosis. These biochemical and histopathological findings are valuable for monitoring health and diagnosing fungal diseases in African catfish.https://dergipark.org.tr/en/download/article-file/4184900african catfishbiochemical parameterspathogen bacteriarhizopus sp. |
| spellingShingle | Alexander Atanasoff Dimitrinka Zapryanova Atanas Bozhkov Çiğdem Ürkü Effects of Bacterial-Fungal Coinfection on Biochemical Parameters and Organ Structure in African Catfish (Clarias gariepinus) Aquatic Sciences and Engineering african catfish biochemical parameters pathogen bacteria rhizopus sp. |
| title | Effects of Bacterial-Fungal Coinfection on Biochemical Parameters and Organ Structure in African Catfish (Clarias gariepinus) |
| title_full | Effects of Bacterial-Fungal Coinfection on Biochemical Parameters and Organ Structure in African Catfish (Clarias gariepinus) |
| title_fullStr | Effects of Bacterial-Fungal Coinfection on Biochemical Parameters and Organ Structure in African Catfish (Clarias gariepinus) |
| title_full_unstemmed | Effects of Bacterial-Fungal Coinfection on Biochemical Parameters and Organ Structure in African Catfish (Clarias gariepinus) |
| title_short | Effects of Bacterial-Fungal Coinfection on Biochemical Parameters and Organ Structure in African Catfish (Clarias gariepinus) |
| title_sort | effects of bacterial fungal coinfection on biochemical parameters and organ structure in african catfish clarias gariepinus |
| topic | african catfish biochemical parameters pathogen bacteria rhizopus sp. |
| url | https://dergipark.org.tr/en/download/article-file/4184900 |
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