Fusidic Acid and Lidocaine-Loaded Electrospun Nanofibers as a Dressing for Accelerated Healing of Infected Wounds
Khulud A Alsulami,1 Abrar A Bakr,1 Alaa Sirwi,2 Mahmoud A Elfaky,2 Rasheed A Shaik,3 Bayan Y Alshehri,1 Alhassan H Aodah,1 Nojoud Al Fayez,1 Abdullah A Alshehri,1 Fahad A Almughem,1 Abdulrahman A Halwani,4,5 Essam A Tawfik1 1Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdula...
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Dove Medical Press
2025-01-01
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| Series: | International Journal of Nanomedicine |
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| author | Alsulami KA Bakr AA Sirwi A Elfaky MA Shaik RA Alshehri BY Aodah AH Al Fayez N Alshehri AA Almughem FA Halwani AA Tawfik EA |
| author_facet | Alsulami KA Bakr AA Sirwi A Elfaky MA Shaik RA Alshehri BY Aodah AH Al Fayez N Alshehri AA Almughem FA Halwani AA Tawfik EA |
| author_sort | Alsulami KA |
| collection | DOAJ |
| description | Khulud A Alsulami,1 Abrar A Bakr,1 Alaa Sirwi,2 Mahmoud A Elfaky,2 Rasheed A Shaik,3 Bayan Y Alshehri,1 Alhassan H Aodah,1 Nojoud Al Fayez,1 Abdullah A Alshehri,1 Fahad A Almughem,1 Abdulrahman A Halwani,4,5 Essam A Tawfik1 1Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology (KACST), Riyadh, 11442, Saudi Arabia; 2Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 4Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 5Regenerative Medicine Unit, King Fahad Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi ArabiaCorrespondence: Essam A Tawfik, Email etawfik@kacst.gov.saIntroduction: Wound treatment is a significant health burden in any healthcare system, which requires proper management to minimize pain and prevent bacterial infections that can complicate the wound healing process.Rationale: There is a need to develop innovative therapies to accelerate wound healing cost-effectively. Herein, two polymer-based nanofibrous systems were developed using poly-lactic-co-glycolic-acid (PLGA) and polyvinylpyrrolidone (PVP) loaded with a combination of an antibiotic (Fusidic acid, FA) and a local anesthetic (Lidocaine, LDC) via electrospinning technique for an expedited healing process by preventing bacterial infections while reducing the pain sensation.Results: The fabricated nanofibers showed an excellent morphology with an average fiber diameter of 556 ± 71 nm and 291 ± 87 nm for the dual drug-loaded PLGA/PVP and PVP nanofibers, respectively. The encapsulation efficiency (EE%) and drug loading (DL) studies revealed that PLGA/PVP loaded with FA and LDC exhibited EE% of 92% and 75%, respectively, while the DL was measured at 40 ± 8 μg/mg for FA and 32 ± 7 μg/mg for LDC. Furthermore, both drugs were fully released from the nanofibers within 48 hours. In contrast, FA/LDC-loaded PVP nanofibers exhibited EE% of 100% for FA and 84% for LDC; DL was measured at 85 ± 3 μg/mg for FA and 70 ± 3 μg/mg for LDC, while both drugs were completely released within 24 hours. The in vitro cytotoxicity study demonstrated a safe concentration of FA and LDC at ≤ 125 μg/mL. The prepared nanofibers were tested in vivo in an S. aureus-infected wound mice model to assess their efficacy, and the results showed that the FA/LDC-PVP had a faster wound closure and the lowest bacterial counts compared to other groups.Conclusion: These findings showed the potential application of the fabricated dual drug-loaded nanofibers as a wound-healing plaster against infected acute wounds.Keywords: electrospinning, nanofibers, fusidic acid, lidocaine, wound healing, infected wound |
| format | Article |
| id | doaj-art-f39f8f51906e4cc58d83126399281ff4 |
| institution | Kabale University |
| issn | 1178-2013 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj-art-f39f8f51906e4cc58d83126399281ff42025-01-21T16:58:06ZengDove Medical PressInternational Journal of Nanomedicine1178-20132025-01-01Volume 2084986999397Fusidic Acid and Lidocaine-Loaded Electrospun Nanofibers as a Dressing for Accelerated Healing of Infected WoundsAlsulami KABakr AASirwi AElfaky MAShaik RAAlshehri BYAodah AHAl Fayez NAlshehri AAAlmughem FAHalwani AATawfik EAKhulud A Alsulami,1 Abrar A Bakr,1 Alaa Sirwi,2 Mahmoud A Elfaky,2 Rasheed A Shaik,3 Bayan Y Alshehri,1 Alhassan H Aodah,1 Nojoud Al Fayez,1 Abdullah A Alshehri,1 Fahad A Almughem,1 Abdulrahman A Halwani,4,5 Essam A Tawfik1 1Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology (KACST), Riyadh, 11442, Saudi Arabia; 2Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 4Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; 5Regenerative Medicine Unit, King Fahad Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi ArabiaCorrespondence: Essam A Tawfik, Email etawfik@kacst.gov.saIntroduction: Wound treatment is a significant health burden in any healthcare system, which requires proper management to minimize pain and prevent bacterial infections that can complicate the wound healing process.Rationale: There is a need to develop innovative therapies to accelerate wound healing cost-effectively. Herein, two polymer-based nanofibrous systems were developed using poly-lactic-co-glycolic-acid (PLGA) and polyvinylpyrrolidone (PVP) loaded with a combination of an antibiotic (Fusidic acid, FA) and a local anesthetic (Lidocaine, LDC) via electrospinning technique for an expedited healing process by preventing bacterial infections while reducing the pain sensation.Results: The fabricated nanofibers showed an excellent morphology with an average fiber diameter of 556 ± 71 nm and 291 ± 87 nm for the dual drug-loaded PLGA/PVP and PVP nanofibers, respectively. The encapsulation efficiency (EE%) and drug loading (DL) studies revealed that PLGA/PVP loaded with FA and LDC exhibited EE% of 92% and 75%, respectively, while the DL was measured at 40 ± 8 μg/mg for FA and 32 ± 7 μg/mg for LDC. Furthermore, both drugs were fully released from the nanofibers within 48 hours. In contrast, FA/LDC-loaded PVP nanofibers exhibited EE% of 100% for FA and 84% for LDC; DL was measured at 85 ± 3 μg/mg for FA and 70 ± 3 μg/mg for LDC, while both drugs were completely released within 24 hours. The in vitro cytotoxicity study demonstrated a safe concentration of FA and LDC at ≤ 125 μg/mL. The prepared nanofibers were tested in vivo in an S. aureus-infected wound mice model to assess their efficacy, and the results showed that the FA/LDC-PVP had a faster wound closure and the lowest bacterial counts compared to other groups.Conclusion: These findings showed the potential application of the fabricated dual drug-loaded nanofibers as a wound-healing plaster against infected acute wounds.Keywords: electrospinning, nanofibers, fusidic acid, lidocaine, wound healing, infected woundhttps://www.dovepress.com/fusidic-acid-and-lidocaine-loaded-electrospun-nanofibers-as-a-dressing-peer-reviewed-fulltext-article-IJNelectrospinningnanofibersfusidic acidlidocainewound healinginfected wound. |
| spellingShingle | Alsulami KA Bakr AA Sirwi A Elfaky MA Shaik RA Alshehri BY Aodah AH Al Fayez N Alshehri AA Almughem FA Halwani AA Tawfik EA Fusidic Acid and Lidocaine-Loaded Electrospun Nanofibers as a Dressing for Accelerated Healing of Infected Wounds International Journal of Nanomedicine electrospinning nanofibers fusidic acid lidocaine wound healing infected wound. |
| title | Fusidic Acid and Lidocaine-Loaded Electrospun Nanofibers as a Dressing for Accelerated Healing of Infected Wounds |
| title_full | Fusidic Acid and Lidocaine-Loaded Electrospun Nanofibers as a Dressing for Accelerated Healing of Infected Wounds |
| title_fullStr | Fusidic Acid and Lidocaine-Loaded Electrospun Nanofibers as a Dressing for Accelerated Healing of Infected Wounds |
| title_full_unstemmed | Fusidic Acid and Lidocaine-Loaded Electrospun Nanofibers as a Dressing for Accelerated Healing of Infected Wounds |
| title_short | Fusidic Acid and Lidocaine-Loaded Electrospun Nanofibers as a Dressing for Accelerated Healing of Infected Wounds |
| title_sort | fusidic acid and lidocaine loaded electrospun nanofibers as a dressing for accelerated healing of infected wounds |
| topic | electrospinning nanofibers fusidic acid lidocaine wound healing infected wound. |
| url | https://www.dovepress.com/fusidic-acid-and-lidocaine-loaded-electrospun-nanofibers-as-a-dressing-peer-reviewed-fulltext-article-IJN |
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