Is an oritavancin catheter lock solution active against biofilms of staphylococci and enterococci?
Background: Oritavancin (ORT) is a new single-dose lipoglycopeptide showing in vitro activity against staphylococci and vancomycin-resistant enterococci. However, there is no data regarding its potential use as a catheter lock solution are scarce. We constructed an in vitro model to analyze the effi...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025002658 |
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| author | Marta Díaz-Navarro Emilia Cercenado Ariadna Monte Andrés Visedo Carmen Rodríguez Ma Jesús Pérez-Granda Patricia Muñoz María Guembe |
| author_facet | Marta Díaz-Navarro Emilia Cercenado Ariadna Monte Andrés Visedo Carmen Rodríguez Ma Jesús Pérez-Granda Patricia Muñoz María Guembe |
| author_sort | Marta Díaz-Navarro |
| collection | DOAJ |
| description | Background: Oritavancin (ORT) is a new single-dose lipoglycopeptide showing in vitro activity against staphylococci and vancomycin-resistant enterococci. However, there is no data regarding its potential use as a catheter lock solution are scarce. We constructed an in vitro model to analyze the efficacy and stability of an ORT lock solution against the biofilm of staphylococci and enterococci over 7 days at 37 °C. Methods: We used Staphylococcus aureus, Staphylococcus epidermidis, and vancomycin-susceptible Enterococcus faecalis ATCC strains. We performed a metabolic activity assay using a 2-mg/ml solution of ORT over a 7-day incubation period at 37 °C. The solution was tested against 24-h biofilms of each strain at day 0 and 7. Metabolic activity was measured using the XTT assay, and median absorbance obtained at 490 nm in the spectrophotometer was compared between day 0 and day 7. Results: The percentage reduction in metabolic activity was 95.3 % between biofilms treated with ORT solution incubated for 7 days and biofilms treated with ORT solution before incubation. Conclusion: Ours is a proof-of-concept study that shows ORT to be a potential treatment as a catheter lock solution for eradication of staphylococcal and E. faecalis biofilms. It is needed to further test ORT against more clinical strains and to compare its activity with other antimicrobials in a biofilm model. |
| format | Article |
| id | doaj-art-f3866d8fcd8a4db18706b5c6b72a59bb |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-f3866d8fcd8a4db18706b5c6b72a59bb2025-02-02T05:28:28ZengElsevierHeliyon2405-84402025-01-01112e41885Is an oritavancin catheter lock solution active against biofilms of staphylococci and enterococci?Marta Díaz-Navarro0Emilia Cercenado1Ariadna Monte2Andrés Visedo3Carmen Rodríguez4Ma Jesús Pérez-Granda5Patricia Muñoz6María Guembe7Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, SpainDepartment of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; School of Medicine, Universidad Complutense de Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Madrid, SpainSchool of Biology, Universidad Complutense de Madrid, SpainDepartment of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, SpainPharmacy Department, Hospital General Universitario Gregorio Marañón, Madrid, SpainDepartment of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; School of Biology, Universidad Complutense de Madrid, SpainDepartment of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; School of Biology, Universidad Complutense de Madrid, Spain; Pharmacy Department, Hospital General Universitario Gregorio Marañón, Madrid, SpainDepartment of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; Corresponding author. Dr. Esquerdo, 46, 28007, Madrid, Spain.Background: Oritavancin (ORT) is a new single-dose lipoglycopeptide showing in vitro activity against staphylococci and vancomycin-resistant enterococci. However, there is no data regarding its potential use as a catheter lock solution are scarce. We constructed an in vitro model to analyze the efficacy and stability of an ORT lock solution against the biofilm of staphylococci and enterococci over 7 days at 37 °C. Methods: We used Staphylococcus aureus, Staphylococcus epidermidis, and vancomycin-susceptible Enterococcus faecalis ATCC strains. We performed a metabolic activity assay using a 2-mg/ml solution of ORT over a 7-day incubation period at 37 °C. The solution was tested against 24-h biofilms of each strain at day 0 and 7. Metabolic activity was measured using the XTT assay, and median absorbance obtained at 490 nm in the spectrophotometer was compared between day 0 and day 7. Results: The percentage reduction in metabolic activity was 95.3 % between biofilms treated with ORT solution incubated for 7 days and biofilms treated with ORT solution before incubation. Conclusion: Ours is a proof-of-concept study that shows ORT to be a potential treatment as a catheter lock solution for eradication of staphylococcal and E. faecalis biofilms. It is needed to further test ORT against more clinical strains and to compare its activity with other antimicrobials in a biofilm model.http://www.sciencedirect.com/science/article/pii/S2405844025002658OritavancinCatheter lock solutionCatheter-related bloodstream infectionActivityStability |
| spellingShingle | Marta Díaz-Navarro Emilia Cercenado Ariadna Monte Andrés Visedo Carmen Rodríguez Ma Jesús Pérez-Granda Patricia Muñoz María Guembe Is an oritavancin catheter lock solution active against biofilms of staphylococci and enterococci? Heliyon Oritavancin Catheter lock solution Catheter-related bloodstream infection Activity Stability |
| title | Is an oritavancin catheter lock solution active against biofilms of staphylococci and enterococci? |
| title_full | Is an oritavancin catheter lock solution active against biofilms of staphylococci and enterococci? |
| title_fullStr | Is an oritavancin catheter lock solution active against biofilms of staphylococci and enterococci? |
| title_full_unstemmed | Is an oritavancin catheter lock solution active against biofilms of staphylococci and enterococci? |
| title_short | Is an oritavancin catheter lock solution active against biofilms of staphylococci and enterococci? |
| title_sort | is an oritavancin catheter lock solution active against biofilms of staphylococci and enterococci |
| topic | Oritavancin Catheter lock solution Catheter-related bloodstream infection Activity Stability |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025002658 |
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