Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model

Lymphocyte cell death contributes to sepsis-induced immunosuppression, leading to poor prognosis. This study examined whether sepsis severity and beta-blocker therapy could affect the degree of T-lymphocyte cell death in a mouse model of sepsis. In the first control study, 20 animals were allocated...

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Main Authors: Takeshi Suzuki, Kei Inoue, Toru Igarashi, Jungo Kato, Hiromasa Nagata, Takashige Yamada, Shizuka Minamishima, Hiroshi Morisaki
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:Critical Care Research and Practice
Online Access:http://dx.doi.org/10.1155/2019/8157482
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author Takeshi Suzuki
Kei Inoue
Toru Igarashi
Jungo Kato
Hiromasa Nagata
Takashige Yamada
Shizuka Minamishima
Hiroshi Morisaki
author_facet Takeshi Suzuki
Kei Inoue
Toru Igarashi
Jungo Kato
Hiromasa Nagata
Takashige Yamada
Shizuka Minamishima
Hiroshi Morisaki
author_sort Takeshi Suzuki
collection DOAJ
description Lymphocyte cell death contributes to sepsis-induced immunosuppression, leading to poor prognosis. This study examined whether sepsis severity and beta-blocker therapy could affect the degree of T-lymphocyte cell death in a mouse model of sepsis. In the first control study, 20 animals were allocated to 4 groups: control group with sham operation (group C, n = 5) and 3 groups with cecum ligation and puncture (CLP) performed at 3 different sites: proximal, middle, and distal cecum (groups CLP-P, CLP-M, and CLP-D, respectively; n = 5 in each group). Their spleens were resected under general anesthesia 24 hours after CLP, and the total number of normal splenic T lymphocytes per mouse and the percentage of apoptotic T lymphocytes were evaluated using flow cytometry. In the second experimental study, the effect of the beta-blocker esmolol was examined in CLP-P (group CLP-PE vs. CLP-P; n = 5 in each group). The total normal splenic T-lymphocyte numbers per mouse significantly decreased in proportion to CLP severity (group C, 18.6 × 106 (15 × 106–23.6 × 106); CLP-D, 9.2 × 106 (8.8 × 106–9.8 × 106); CLP-M, 6.7 × 106 (6.3 × 106–7.0 × 106); and CLP-P, 5.3 × 106 (5.1 × 106–6.8 × 106)). Beta-blocker therapy restored T-lymphocyte numbers (group CLP-PE vs. CLP-P; 6.94 ± 1.52 × 106 vs. 4.18 ± 1.71 × 106; p=0.027) without affecting apoptosis percentage. Beta-blocker therapy might improve sepsis-induced immunosuppression via normal splenic T-lymphocyte preservation.
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spelling doaj-art-f360aa836a974ec99f06dc762da5ba8c2025-02-03T06:01:53ZengWileyCritical Care Research and Practice2090-13052090-13132019-01-01201910.1155/2019/81574828157482Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis ModelTakeshi Suzuki0Kei Inoue1Toru Igarashi2Jungo Kato3Hiromasa Nagata4Takashige Yamada5Shizuka Minamishima6Hiroshi Morisaki7Tokai University School of Medicine, Department of Anesthesiology, 143 Shimokasuya, Isehara, Kanagawa 259-1193, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanLymphocyte cell death contributes to sepsis-induced immunosuppression, leading to poor prognosis. This study examined whether sepsis severity and beta-blocker therapy could affect the degree of T-lymphocyte cell death in a mouse model of sepsis. In the first control study, 20 animals were allocated to 4 groups: control group with sham operation (group C, n = 5) and 3 groups with cecum ligation and puncture (CLP) performed at 3 different sites: proximal, middle, and distal cecum (groups CLP-P, CLP-M, and CLP-D, respectively; n = 5 in each group). Their spleens were resected under general anesthesia 24 hours after CLP, and the total number of normal splenic T lymphocytes per mouse and the percentage of apoptotic T lymphocytes were evaluated using flow cytometry. In the second experimental study, the effect of the beta-blocker esmolol was examined in CLP-P (group CLP-PE vs. CLP-P; n = 5 in each group). The total normal splenic T-lymphocyte numbers per mouse significantly decreased in proportion to CLP severity (group C, 18.6 × 106 (15 × 106–23.6 × 106); CLP-D, 9.2 × 106 (8.8 × 106–9.8 × 106); CLP-M, 6.7 × 106 (6.3 × 106–7.0 × 106); and CLP-P, 5.3 × 106 (5.1 × 106–6.8 × 106)). Beta-blocker therapy restored T-lymphocyte numbers (group CLP-PE vs. CLP-P; 6.94 ± 1.52 × 106 vs. 4.18 ± 1.71 × 106; p=0.027) without affecting apoptosis percentage. Beta-blocker therapy might improve sepsis-induced immunosuppression via normal splenic T-lymphocyte preservation.http://dx.doi.org/10.1155/2019/8157482
spellingShingle Takeshi Suzuki
Kei Inoue
Toru Igarashi
Jungo Kato
Hiromasa Nagata
Takashige Yamada
Shizuka Minamishima
Hiroshi Morisaki
Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model
Critical Care Research and Practice
title Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model
title_full Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model
title_fullStr Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model
title_full_unstemmed Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model
title_short Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model
title_sort beta blocker therapy preserves normal splenic t lymphocyte numbers reduced in proportion to sepsis severity in a sepsis model
url http://dx.doi.org/10.1155/2019/8157482
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