Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model
Lymphocyte cell death contributes to sepsis-induced immunosuppression, leading to poor prognosis. This study examined whether sepsis severity and beta-blocker therapy could affect the degree of T-lymphocyte cell death in a mouse model of sepsis. In the first control study, 20 animals were allocated...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Critical Care Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2019/8157482 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551294825398272 |
|---|---|
| author | Takeshi Suzuki Kei Inoue Toru Igarashi Jungo Kato Hiromasa Nagata Takashige Yamada Shizuka Minamishima Hiroshi Morisaki |
| author_facet | Takeshi Suzuki Kei Inoue Toru Igarashi Jungo Kato Hiromasa Nagata Takashige Yamada Shizuka Minamishima Hiroshi Morisaki |
| author_sort | Takeshi Suzuki |
| collection | DOAJ |
| description | Lymphocyte cell death contributes to sepsis-induced immunosuppression, leading to poor prognosis. This study examined whether sepsis severity and beta-blocker therapy could affect the degree of T-lymphocyte cell death in a mouse model of sepsis. In the first control study, 20 animals were allocated to 4 groups: control group with sham operation (group C, n = 5) and 3 groups with cecum ligation and puncture (CLP) performed at 3 different sites: proximal, middle, and distal cecum (groups CLP-P, CLP-M, and CLP-D, respectively; n = 5 in each group). Their spleens were resected under general anesthesia 24 hours after CLP, and the total number of normal splenic T lymphocytes per mouse and the percentage of apoptotic T lymphocytes were evaluated using flow cytometry. In the second experimental study, the effect of the beta-blocker esmolol was examined in CLP-P (group CLP-PE vs. CLP-P; n = 5 in each group). The total normal splenic T-lymphocyte numbers per mouse significantly decreased in proportion to CLP severity (group C, 18.6 × 106 (15 × 106–23.6 × 106); CLP-D, 9.2 × 106 (8.8 × 106–9.8 × 106); CLP-M, 6.7 × 106 (6.3 × 106–7.0 × 106); and CLP-P, 5.3 × 106 (5.1 × 106–6.8 × 106)). Beta-blocker therapy restored T-lymphocyte numbers (group CLP-PE vs. CLP-P; 6.94 ± 1.52 × 106 vs. 4.18 ± 1.71 × 106; p=0.027) without affecting apoptosis percentage. Beta-blocker therapy might improve sepsis-induced immunosuppression via normal splenic T-lymphocyte preservation. |
| format | Article |
| id | doaj-art-f360aa836a974ec99f06dc762da5ba8c |
| institution | Kabale University |
| issn | 2090-1305 2090-1313 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Critical Care Research and Practice |
| spelling | doaj-art-f360aa836a974ec99f06dc762da5ba8c2025-02-03T06:01:53ZengWileyCritical Care Research and Practice2090-13052090-13132019-01-01201910.1155/2019/81574828157482Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis ModelTakeshi Suzuki0Kei Inoue1Toru Igarashi2Jungo Kato3Hiromasa Nagata4Takashige Yamada5Shizuka Minamishima6Hiroshi Morisaki7Tokai University School of Medicine, Department of Anesthesiology, 143 Shimokasuya, Isehara, Kanagawa 259-1193, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanKeio University School of Medicine, Department of Anesthesiology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, JapanLymphocyte cell death contributes to sepsis-induced immunosuppression, leading to poor prognosis. This study examined whether sepsis severity and beta-blocker therapy could affect the degree of T-lymphocyte cell death in a mouse model of sepsis. In the first control study, 20 animals were allocated to 4 groups: control group with sham operation (group C, n = 5) and 3 groups with cecum ligation and puncture (CLP) performed at 3 different sites: proximal, middle, and distal cecum (groups CLP-P, CLP-M, and CLP-D, respectively; n = 5 in each group). Their spleens were resected under general anesthesia 24 hours after CLP, and the total number of normal splenic T lymphocytes per mouse and the percentage of apoptotic T lymphocytes were evaluated using flow cytometry. In the second experimental study, the effect of the beta-blocker esmolol was examined in CLP-P (group CLP-PE vs. CLP-P; n = 5 in each group). The total normal splenic T-lymphocyte numbers per mouse significantly decreased in proportion to CLP severity (group C, 18.6 × 106 (15 × 106–23.6 × 106); CLP-D, 9.2 × 106 (8.8 × 106–9.8 × 106); CLP-M, 6.7 × 106 (6.3 × 106–7.0 × 106); and CLP-P, 5.3 × 106 (5.1 × 106–6.8 × 106)). Beta-blocker therapy restored T-lymphocyte numbers (group CLP-PE vs. CLP-P; 6.94 ± 1.52 × 106 vs. 4.18 ± 1.71 × 106; p=0.027) without affecting apoptosis percentage. Beta-blocker therapy might improve sepsis-induced immunosuppression via normal splenic T-lymphocyte preservation.http://dx.doi.org/10.1155/2019/8157482 |
| spellingShingle | Takeshi Suzuki Kei Inoue Toru Igarashi Jungo Kato Hiromasa Nagata Takashige Yamada Shizuka Minamishima Hiroshi Morisaki Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model Critical Care Research and Practice |
| title | Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model |
| title_full | Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model |
| title_fullStr | Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model |
| title_full_unstemmed | Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model |
| title_short | Beta-Blocker Therapy Preserves Normal Splenic T-Lymphocyte Numbers Reduced in Proportion to Sepsis Severity in a Sepsis Model |
| title_sort | beta blocker therapy preserves normal splenic t lymphocyte numbers reduced in proportion to sepsis severity in a sepsis model |
| url | http://dx.doi.org/10.1155/2019/8157482 |
| work_keys_str_mv | AT takeshisuzuki betablockertherapypreservesnormalsplenictlymphocytenumbersreducedinproportiontosepsisseverityinasepsismodel AT keiinoue betablockertherapypreservesnormalsplenictlymphocytenumbersreducedinproportiontosepsisseverityinasepsismodel AT toruigarashi betablockertherapypreservesnormalsplenictlymphocytenumbersreducedinproportiontosepsisseverityinasepsismodel AT jungokato betablockertherapypreservesnormalsplenictlymphocytenumbersreducedinproportiontosepsisseverityinasepsismodel AT hiromasanagata betablockertherapypreservesnormalsplenictlymphocytenumbersreducedinproportiontosepsisseverityinasepsismodel AT takashigeyamada betablockertherapypreservesnormalsplenictlymphocytenumbersreducedinproportiontosepsisseverityinasepsismodel AT shizukaminamishima betablockertherapypreservesnormalsplenictlymphocytenumbersreducedinproportiontosepsisseverityinasepsismodel AT hiroshimorisaki betablockertherapypreservesnormalsplenictlymphocytenumbersreducedinproportiontosepsisseverityinasepsismodel |