Applications of piggyBac Transposons for Genome Manipulation in Stem Cells
Transposons are mobile genetic elements in the genome. The piggyBac (PB) transposon system is increasingly being used for stem cell research due to its high transposition efficiency and seamless excision capacity. Over the past few decades, forward genetic screens based on PB transposons have been s...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/3829286 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832565966185168896 |
|---|---|
| author | Yi Sun Guang Liu Yue Huang |
| author_facet | Yi Sun Guang Liu Yue Huang |
| author_sort | Yi Sun |
| collection | DOAJ |
| description | Transposons are mobile genetic elements in the genome. The piggyBac (PB) transposon system is increasingly being used for stem cell research due to its high transposition efficiency and seamless excision capacity. Over the past few decades, forward genetic screens based on PB transposons have been successfully established to identify genes associated with drug resistance and stem cell-related characteristics. Moreover, PB transposon is regarded as a promising gene therapy vector and has been used in some clinically relevant stem cells. Here, we review the recent progress on the basic biology of PB, highlight its applications in current stem cell research, and discuss its advantages and challenges. |
| format | Article |
| id | doaj-art-f3521bb5df494459a6bf5a7f9e55629c |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-f3521bb5df494459a6bf5a7f9e55629c2025-02-03T01:05:32ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/38292863829286Applications of piggyBac Transposons for Genome Manipulation in Stem CellsYi Sun0Guang Liu1Yue Huang2State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, ChinaState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, ChinaState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, ChinaTransposons are mobile genetic elements in the genome. The piggyBac (PB) transposon system is increasingly being used for stem cell research due to its high transposition efficiency and seamless excision capacity. Over the past few decades, forward genetic screens based on PB transposons have been successfully established to identify genes associated with drug resistance and stem cell-related characteristics. Moreover, PB transposon is regarded as a promising gene therapy vector and has been used in some clinically relevant stem cells. Here, we review the recent progress on the basic biology of PB, highlight its applications in current stem cell research, and discuss its advantages and challenges.http://dx.doi.org/10.1155/2021/3829286 |
| spellingShingle | Yi Sun Guang Liu Yue Huang Applications of piggyBac Transposons for Genome Manipulation in Stem Cells Stem Cells International |
| title | Applications of piggyBac Transposons for Genome Manipulation in Stem Cells |
| title_full | Applications of piggyBac Transposons for Genome Manipulation in Stem Cells |
| title_fullStr | Applications of piggyBac Transposons for Genome Manipulation in Stem Cells |
| title_full_unstemmed | Applications of piggyBac Transposons for Genome Manipulation in Stem Cells |
| title_short | Applications of piggyBac Transposons for Genome Manipulation in Stem Cells |
| title_sort | applications of piggybac transposons for genome manipulation in stem cells |
| url | http://dx.doi.org/10.1155/2021/3829286 |
| work_keys_str_mv | AT yisun applicationsofpiggybactransposonsforgenomemanipulationinstemcells AT guangliu applicationsofpiggybactransposonsforgenomemanipulationinstemcells AT yuehuang applicationsofpiggybactransposonsforgenomemanipulationinstemcells |