Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand
Abstract The evolution of genetic diversity and population structure of Plasmodium vivax as malaria elimination approaches remains unclear. This study analyzed the genetic variation and molecular epidemiology of P. vivax from Yala Province in southern Thailand, an area in the pre-elimination phase....
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-86578-8 |
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| author | Parsakorn Tapaopong Gustavo da Silva Aurel Holzschuh Wasinee Rungsarityotin Chayanut Suansomjit Kanit Pumchuea Khajohnpong Manopwisedjaroen Amnat Khamsiriwatchara Podjadeach Khuntong Liwang Cui Cristian Koepfli Jetsumon Sattabongkot Wang Nguitragool |
| author_facet | Parsakorn Tapaopong Gustavo da Silva Aurel Holzschuh Wasinee Rungsarityotin Chayanut Suansomjit Kanit Pumchuea Khajohnpong Manopwisedjaroen Amnat Khamsiriwatchara Podjadeach Khuntong Liwang Cui Cristian Koepfli Jetsumon Sattabongkot Wang Nguitragool |
| author_sort | Parsakorn Tapaopong |
| collection | DOAJ |
| description | Abstract The evolution of genetic diversity and population structure of Plasmodium vivax as malaria elimination approaches remains unclear. This study analyzed the genetic variation and molecular epidemiology of P. vivax from Yala Province in southern Thailand, an area in the pre-elimination phase. Seventy P. vivax isolates, collected between 2017 and 2020, were genotyped for domain II of pvdbp and the 42-kDa region of pvmsp1 using amplicon deep sequencing. Data from Yala province were compared to published data from Tak province, where transmission was higher. Key analyses included nucleotide diversity (π), haplotype diversity (Hd), natural selection, recombination rates, and complexity of infection (COI). Genetic diversity in Yala was relatively low (π = 0.008dbp and 0.014msp1; Hd = 0.774dbp and 0.407msp1) compared to Tak (π = 0.012dbp and 0.027msp1; Hd = 0.849dbp and 0.962msp1). In Yala, polyclonal infections were found in 53.7% of pvdbp II and 47.8% of pvmsp1 42 isolates, with average COI of 1.6 and 1.7. Both genes were under balancing selection. Distinct genetic differences were found between Yala and Tak in pvmsp1 42 , providing a local genotypic profile useful for tracing parasite origins. |
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| id | doaj-art-f308c83d8ddc4d1aa5c1923a56d34b10 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-f308c83d8ddc4d1aa5c1923a56d34b102025-01-26T12:33:57ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-025-86578-8Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern ThailandParsakorn Tapaopong0Gustavo da Silva1Aurel Holzschuh2Wasinee Rungsarityotin3Chayanut Suansomjit4Kanit Pumchuea5Khajohnpong Manopwisedjaroen6Amnat Khamsiriwatchara7Podjadeach Khuntong8Liwang Cui9Cristian Koepfli10Jetsumon Sattabongkot11Wang Nguitragool12Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Biological Sciences, Eck Institute for Global Health, Galvin Life Sciences, University of Notre DameDepartment of Medicine, Swiss Tropical and Public Health InstituteMahidol University Frontier Research Facility, Mahidol UniversityMahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol UniversityMahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol UniversityMahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol UniversityFaculty of Tropical Medicine, Center of Excellence for Biomedical and Public Health Informatics (BIOPHICS), Mahidol UniversityFaculty of Tropical Medicine, Center of Excellence for Biomedical and Public Health Informatics (BIOPHICS), Mahidol UniversityDivision of Infectious Diseases and International Medicine, Department of Internal Medicine, Morsani College of Medicine, University of South FloridaDepartment of Biological Sciences, Eck Institute for Global Health, Galvin Life Sciences, University of Notre DameMahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol UniversityMahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol UniversityAbstract The evolution of genetic diversity and population structure of Plasmodium vivax as malaria elimination approaches remains unclear. This study analyzed the genetic variation and molecular epidemiology of P. vivax from Yala Province in southern Thailand, an area in the pre-elimination phase. Seventy P. vivax isolates, collected between 2017 and 2020, were genotyped for domain II of pvdbp and the 42-kDa region of pvmsp1 using amplicon deep sequencing. Data from Yala province were compared to published data from Tak province, where transmission was higher. Key analyses included nucleotide diversity (π), haplotype diversity (Hd), natural selection, recombination rates, and complexity of infection (COI). Genetic diversity in Yala was relatively low (π = 0.008dbp and 0.014msp1; Hd = 0.774dbp and 0.407msp1) compared to Tak (π = 0.012dbp and 0.027msp1; Hd = 0.849dbp and 0.962msp1). In Yala, polyclonal infections were found in 53.7% of pvdbp II and 47.8% of pvmsp1 42 isolates, with average COI of 1.6 and 1.7. Both genes were under balancing selection. Distinct genetic differences were found between Yala and Tak in pvmsp1 42 , providing a local genotypic profile useful for tracing parasite origins.https://doi.org/10.1038/s41598-025-86578-8MalariaPlasmodium vivaxGenetic diversityAmplicon sequencingDuffy binding proteinMerozoite surface protein-1 |
| spellingShingle | Parsakorn Tapaopong Gustavo da Silva Aurel Holzschuh Wasinee Rungsarityotin Chayanut Suansomjit Kanit Pumchuea Khajohnpong Manopwisedjaroen Amnat Khamsiriwatchara Podjadeach Khuntong Liwang Cui Cristian Koepfli Jetsumon Sattabongkot Wang Nguitragool Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand Scientific Reports Malaria Plasmodium vivax Genetic diversity Amplicon sequencing Duffy binding protein Merozoite surface protein-1 |
| title | Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand |
| title_full | Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand |
| title_fullStr | Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand |
| title_full_unstemmed | Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand |
| title_short | Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand |
| title_sort | molecular epidemiology and genetic diversity of disappearing plasmodium vivax in southern thailand |
| topic | Malaria Plasmodium vivax Genetic diversity Amplicon sequencing Duffy binding protein Merozoite surface protein-1 |
| url | https://doi.org/10.1038/s41598-025-86578-8 |
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