Increased spontaneous recombination in RNase H2-deficient cells arises from multiple contiguous rNMPs and not from single rNMP residues incorporated by DNA polymerase epsilon
Ribonucleotides can become embedded in DNA from insertion by DNA polymerases, failure to remove Okazaki fragment primers, R-loops that can prime replication, and RNA/cDNA-mediated recombination. RNA:DNA hybrids are removed by RNase H enzymes. Single rNMPs in DNA are removed by RNase H2 and if they r...
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Shared Science Publishers OG
2016-05-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/increased-spontaneous-recombination-in-rnase-h2-deficient-cells-arises-from-multiple-contiguous-rnmps-and-not-from-single-rnmp-residues-incorporated-by-dna-polymerase-epsilon/ |
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| author | Anastasiya Epshtein Catherine J. Potenski Hannah L. Klein |
| author_facet | Anastasiya Epshtein Catherine J. Potenski Hannah L. Klein |
| author_sort | Anastasiya Epshtein |
| collection | DOAJ |
| description | Ribonucleotides can become embedded in DNA from insertion by DNA polymerases, failure to remove Okazaki fragment primers, R-loops that can prime replication, and RNA/cDNA-mediated recombination. RNA:DNA hybrids are removed by RNase H enzymes. Single rNMPs in DNA are removed by RNase H2 and if they remain on the leading strand, can lead to mutagenesis in a Top1-dependent pathway. rNMPs in DNA can also stimulate genome instability, among which are homologous recombination gene conversion events. We previously found that, similar to the rNMP-stimulated mutagenesis, rNMP-stimulated recombination was also Top1-dependent. However, in contrast to mutagenesis, we report here that recombination is not stimulated by rNMPs incorporated by the replicative polymerase epsilon. Instead, recombination seems to be stimulated by multiple contiguous rNMPs, which may arise from R-loops or replication priming events. |
| format | Article |
| id | doaj-art-f2e2ef1fe5834c0887e8c7d74d18c01c |
| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2016-05-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-f2e2ef1fe5834c0887e8c7d74d18c01c2025-08-20T02:53:21ZengShared Science Publishers OGMicrobial Cell2311-26382016-05-013624825410.15698/mic2016.06.506Increased spontaneous recombination in RNase H2-deficient cells arises from multiple contiguous rNMPs and not from single rNMP residues incorporated by DNA polymerase epsilonAnastasiya Epshtein0Catherine J. Potenski1Hannah L. Klein2Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 550 First Avenue, New York, New York 10016, USA.Nature Publishing Group, One New York Plaza, New York, New York 10004, USA.Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 550 First Avenue, New York, New York 10016, USA.Ribonucleotides can become embedded in DNA from insertion by DNA polymerases, failure to remove Okazaki fragment primers, R-loops that can prime replication, and RNA/cDNA-mediated recombination. RNA:DNA hybrids are removed by RNase H enzymes. Single rNMPs in DNA are removed by RNase H2 and if they remain on the leading strand, can lead to mutagenesis in a Top1-dependent pathway. rNMPs in DNA can also stimulate genome instability, among which are homologous recombination gene conversion events. We previously found that, similar to the rNMP-stimulated mutagenesis, rNMP-stimulated recombination was also Top1-dependent. However, in contrast to mutagenesis, we report here that recombination is not stimulated by rNMPs incorporated by the replicative polymerase epsilon. Instead, recombination seems to be stimulated by multiple contiguous rNMPs, which may arise from R-loops or replication priming events.http://microbialcell.com/researcharticles/increased-spontaneous-recombination-in-rnase-h2-deficient-cells-arises-from-multiple-contiguous-rnmps-and-not-from-single-rnmp-residues-incorporated-by-dna-polymerase-epsilon/RNase H2 recombination rNMPDNA polymerase |
| spellingShingle | Anastasiya Epshtein Catherine J. Potenski Hannah L. Klein Increased spontaneous recombination in RNase H2-deficient cells arises from multiple contiguous rNMPs and not from single rNMP residues incorporated by DNA polymerase epsilon Microbial Cell RNase H2 recombination rNMP DNA polymerase |
| title | Increased spontaneous recombination in RNase H2-deficient cells arises from multiple contiguous rNMPs and not from single rNMP residues incorporated by DNA polymerase epsilon |
| title_full | Increased spontaneous recombination in RNase H2-deficient cells arises from multiple contiguous rNMPs and not from single rNMP residues incorporated by DNA polymerase epsilon |
| title_fullStr | Increased spontaneous recombination in RNase H2-deficient cells arises from multiple contiguous rNMPs and not from single rNMP residues incorporated by DNA polymerase epsilon |
| title_full_unstemmed | Increased spontaneous recombination in RNase H2-deficient cells arises from multiple contiguous rNMPs and not from single rNMP residues incorporated by DNA polymerase epsilon |
| title_short | Increased spontaneous recombination in RNase H2-deficient cells arises from multiple contiguous rNMPs and not from single rNMP residues incorporated by DNA polymerase epsilon |
| title_sort | increased spontaneous recombination in rnase h2 deficient cells arises from multiple contiguous rnmps and not from single rnmp residues incorporated by dna polymerase epsilon |
| topic | RNase H2 recombination rNMP DNA polymerase |
| url | http://microbialcell.com/researcharticles/increased-spontaneous-recombination-in-rnase-h2-deficient-cells-arises-from-multiple-contiguous-rnmps-and-not-from-single-rnmp-residues-incorporated-by-dna-polymerase-epsilon/ |
| work_keys_str_mv | AT anastasiyaepshtein increasedspontaneousrecombinationinrnaseh2deficientcellsarisesfrommultiplecontiguousrnmpsandnotfromsinglernmpresiduesincorporatedbydnapolymeraseepsilon AT catherinejpotenski increasedspontaneousrecombinationinrnaseh2deficientcellsarisesfrommultiplecontiguousrnmpsandnotfromsinglernmpresiduesincorporatedbydnapolymeraseepsilon AT hannahlklein increasedspontaneousrecombinationinrnaseh2deficientcellsarisesfrommultiplecontiguousrnmpsandnotfromsinglernmpresiduesincorporatedbydnapolymeraseepsilon |