Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization
To achieve safer patient treatments, serum-free cell culture conditions have to be established for cell therapies. In previous studies, we demonstrated that serum-free culture favored the proliferation of MSCA-1+ osteoprogenitors derived from the jaw periosteum. In this study, the in vitro formation...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/1651376 |
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| author | Eva Brauchle Daniel Carvajal Berrio Melanie Rieger Katja Schenke-Layland Siegmar Reinert Dorothea Alexander |
| author_facet | Eva Brauchle Daniel Carvajal Berrio Melanie Rieger Katja Schenke-Layland Siegmar Reinert Dorothea Alexander |
| author_sort | Eva Brauchle |
| collection | DOAJ |
| description | To achieve safer patient treatments, serum-free cell culture conditions have to be established for cell therapies. In previous studies, we demonstrated that serum-free culture favored the proliferation of MSCA-1+ osteoprogenitors derived from the jaw periosteum. In this study, the in vitro formation of bone-specific matrix by MSCA-1+ jaw periosteal cells (JPCs, 3 donors) was assessed and compared under serum-free and serum-containing media conditions using the marker-free Raman spectroscopy. Based on a standard fluorescence assay, JPCs from one patient were not able to mineralize under serum-containing culture conditions, whereas the other cells showed similar mineralization levels under both conditions. Raman spectra from mineralizing MSCA-1+ JPCs revealed higher levels of hydroxyapatite formation and higher mineral to matrix ratios under serum-free culture conditions. Higher carbonate to phosphate ratios and higher crystallinity in JPCs cultured under serum-containing conditions indicated immature bone formation. Due to reduced collagen production under serum-free conditions, we obtained significant differences in collagen maturity and proline to hydroxyproline ratios compared to serum-free conditions. We conclude that Raman spectroscopy is a useful tool for the assessment and noninvasive monitoring of in vitro mineralization of osteoprogenitor cells. Further studies should extend this knowledge and improve JPC mineralization by optimizing culture conditions. |
| format | Article |
| id | doaj-art-f2d0e9a91e9b427989b480bef5e6cba5 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-f2d0e9a91e9b427989b480bef5e6cba52025-02-03T01:21:18ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/16513761651376Raman Spectroscopic Analyses of Jaw Periosteal Cell MineralizationEva Brauchle0Daniel Carvajal Berrio1Melanie Rieger2Katja Schenke-Layland3Siegmar Reinert4Dorothea Alexander5Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Department of Cell and Tissue Engineering, Nobelstr. 12, 70569 Stuttgart, GermanyDepartment of Women’s Health, Research Institute for Women’s Health, Eberhard Karls University, Tübingen, Silcherstr. 7/1, 72076 Tübingen, GermanyDepartment of Oral and Maxillofacial Surgery, University Hospital of Tübingen, 72076 Tübingen, GermanyFraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Department of Cell and Tissue Engineering, Nobelstr. 12, 70569 Stuttgart, GermanyDepartment of Oral and Maxillofacial Surgery, University Hospital of Tübingen, 72076 Tübingen, GermanyDepartment of Oral and Maxillofacial Surgery, University Hospital of Tübingen, 72076 Tübingen, GermanyTo achieve safer patient treatments, serum-free cell culture conditions have to be established for cell therapies. In previous studies, we demonstrated that serum-free culture favored the proliferation of MSCA-1+ osteoprogenitors derived from the jaw periosteum. In this study, the in vitro formation of bone-specific matrix by MSCA-1+ jaw periosteal cells (JPCs, 3 donors) was assessed and compared under serum-free and serum-containing media conditions using the marker-free Raman spectroscopy. Based on a standard fluorescence assay, JPCs from one patient were not able to mineralize under serum-containing culture conditions, whereas the other cells showed similar mineralization levels under both conditions. Raman spectra from mineralizing MSCA-1+ JPCs revealed higher levels of hydroxyapatite formation and higher mineral to matrix ratios under serum-free culture conditions. Higher carbonate to phosphate ratios and higher crystallinity in JPCs cultured under serum-containing conditions indicated immature bone formation. Due to reduced collagen production under serum-free conditions, we obtained significant differences in collagen maturity and proline to hydroxyproline ratios compared to serum-free conditions. We conclude that Raman spectroscopy is a useful tool for the assessment and noninvasive monitoring of in vitro mineralization of osteoprogenitor cells. Further studies should extend this knowledge and improve JPC mineralization by optimizing culture conditions.http://dx.doi.org/10.1155/2017/1651376 |
| spellingShingle | Eva Brauchle Daniel Carvajal Berrio Melanie Rieger Katja Schenke-Layland Siegmar Reinert Dorothea Alexander Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization Stem Cells International |
| title | Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization |
| title_full | Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization |
| title_fullStr | Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization |
| title_full_unstemmed | Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization |
| title_short | Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization |
| title_sort | raman spectroscopic analyses of jaw periosteal cell mineralization |
| url | http://dx.doi.org/10.1155/2017/1651376 |
| work_keys_str_mv | AT evabrauchle ramanspectroscopicanalysesofjawperiostealcellmineralization AT danielcarvajalberrio ramanspectroscopicanalysesofjawperiostealcellmineralization AT melanierieger ramanspectroscopicanalysesofjawperiostealcellmineralization AT katjaschenkelayland ramanspectroscopicanalysesofjawperiostealcellmineralization AT siegmarreinert ramanspectroscopicanalysesofjawperiostealcellmineralization AT dorotheaalexander ramanspectroscopicanalysesofjawperiostealcellmineralization |