Molecular Identification of Mycobacterium Species of Public Health and Veterinary Importance from Cattle in the South State of México
Mycobacterium genus causes a variety of zoonotic diseases. The best known example is the zoonotic tuberculosis due to M. bovis. Much less is known about “nontuberculous mycobacteria (NTM),” which are also associated with infections in humans. The Mexican standard NOM-ZOO-031-1995 regulates the prese...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/6094587 |
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| author | Adrian Zaragoza Bastida Nallely Rivero Pérez Benjamín Valladares Carranza Keila Isaac-Olivé Pablo Moreno Pérez Horacio Sandoval Trujillo Ninfa Ramírez Durán |
| author_facet | Adrian Zaragoza Bastida Nallely Rivero Pérez Benjamín Valladares Carranza Keila Isaac-Olivé Pablo Moreno Pérez Horacio Sandoval Trujillo Ninfa Ramírez Durán |
| author_sort | Adrian Zaragoza Bastida |
| collection | DOAJ |
| description | Mycobacterium genus causes a variety of zoonotic diseases. The best known example is the zoonotic tuberculosis due to M. bovis. Much less is known about “nontuberculous mycobacteria (NTM),” which are also associated with infections in humans. The Mexican standard NOM-ZOO-031-1995 regulates the presence of M. bovis in cattle; however, no regulation exists for the NTM species. The objective of this study was to isolate and identify nontuberculous mycobacteria species from cattle of local herds in the south region of the State of Mexico through the identification and detection of the 100 bp molecular marker in the 23S rRNA gene with subsequent sequencing of the 16S rRNA gene. Milk samples (35) and nasal exudate samples (68) were collected. From the 108 strains isolated, 39 were selected for identification. Thirteen strains isolated from nasal exudates amplified the 100 bp molecular marker and were identified as M. neoaurum (six strains), M. parafortuitum (four strains), M. moriokaense (two strains), and M. confluentis (one strain). Except M. parafortuitum, the other species identified are of public health and veterinary concern because they are pathogenic to humans, especially those with underlying medical conditions. |
| format | Article |
| id | doaj-art-f2ca8fa5084f4677bba876b7bf9a9065 |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-f2ca8fa5084f4677bba876b7bf9a90652025-02-03T01:20:46ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932017-01-01201710.1155/2017/60945876094587Molecular Identification of Mycobacterium Species of Public Health and Veterinary Importance from Cattle in the South State of MéxicoAdrian Zaragoza Bastida0Nallely Rivero Pérez1Benjamín Valladares Carranza2Keila Isaac-Olivé3Pablo Moreno Pérez4Horacio Sandoval Trujillo5Ninfa Ramírez Durán6Facultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan/Jesús Carranza s/n, 50180 Toluca, MEX, MexicoÁrea Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuaria, Universidad Autónoma del Estado de Hidalgo, Av. Universidad Km 1, Ex-Hda. de Aquetzalpa, 43600 Tulancingo, HGO, MexicoCentro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Panamericana Toluca-Atlacomulco, 50200 Toluca, MEX, MexicoFacultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan/Jesús Carranza s/n, 50180 Toluca, MEX, MexicoFacultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan/Jesús Carranza s/n, 50180 Toluca, MEX, MexicoDepartamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, Calzada del Hueso 1100, 04960 Ciudad de México, MexicoFacultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan/Jesús Carranza s/n, 50180 Toluca, MEX, MexicoMycobacterium genus causes a variety of zoonotic diseases. The best known example is the zoonotic tuberculosis due to M. bovis. Much less is known about “nontuberculous mycobacteria (NTM),” which are also associated with infections in humans. The Mexican standard NOM-ZOO-031-1995 regulates the presence of M. bovis in cattle; however, no regulation exists for the NTM species. The objective of this study was to isolate and identify nontuberculous mycobacteria species from cattle of local herds in the south region of the State of Mexico through the identification and detection of the 100 bp molecular marker in the 23S rRNA gene with subsequent sequencing of the 16S rRNA gene. Milk samples (35) and nasal exudate samples (68) were collected. From the 108 strains isolated, 39 were selected for identification. Thirteen strains isolated from nasal exudates amplified the 100 bp molecular marker and were identified as M. neoaurum (six strains), M. parafortuitum (four strains), M. moriokaense (two strains), and M. confluentis (one strain). Except M. parafortuitum, the other species identified are of public health and veterinary concern because they are pathogenic to humans, especially those with underlying medical conditions.http://dx.doi.org/10.1155/2017/6094587 |
| spellingShingle | Adrian Zaragoza Bastida Nallely Rivero Pérez Benjamín Valladares Carranza Keila Isaac-Olivé Pablo Moreno Pérez Horacio Sandoval Trujillo Ninfa Ramírez Durán Molecular Identification of Mycobacterium Species of Public Health and Veterinary Importance from Cattle in the South State of México Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Molecular Identification of Mycobacterium Species of Public Health and Veterinary Importance from Cattle in the South State of México |
| title_full | Molecular Identification of Mycobacterium Species of Public Health and Veterinary Importance from Cattle in the South State of México |
| title_fullStr | Molecular Identification of Mycobacterium Species of Public Health and Veterinary Importance from Cattle in the South State of México |
| title_full_unstemmed | Molecular Identification of Mycobacterium Species of Public Health and Veterinary Importance from Cattle in the South State of México |
| title_short | Molecular Identification of Mycobacterium Species of Public Health and Veterinary Importance from Cattle in the South State of México |
| title_sort | molecular identification of mycobacterium species of public health and veterinary importance from cattle in the south state of mexico |
| url | http://dx.doi.org/10.1155/2017/6094587 |
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