Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury
Tomatidine, which is isolated from green tomato, can ameliorate inflammation and oxidative stress in cells and animal experiments and has been shown to improve airway inflammation in a murine model of asthma. Here, we investigated whether tomatidine can ameliorate acute lung injury in mice. Mice wer...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/4544294 |
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| author | Wen-Chung Huang Shu-Ju Wu Ya-Ling Chen Chwan-Fwu Lin Chian-Jiun Liou |
| author_facet | Wen-Chung Huang Shu-Ju Wu Ya-Ling Chen Chwan-Fwu Lin Chian-Jiun Liou |
| author_sort | Wen-Chung Huang |
| collection | DOAJ |
| description | Tomatidine, which is isolated from green tomato, can ameliorate inflammation and oxidative stress in cells and animal experiments and has been shown to improve airway inflammation in a murine model of asthma. Here, we investigated whether tomatidine can ameliorate acute lung injury in mice. Mice were given tomatidine by intraperitoneal injection for 7 consecutive days, and then, lung injury was induced via intratracheal instillation of lipopolysaccharide (LPS). Tomatidine reduced inflammatory cytokine expressions in bronchoalveolar lavage fluid (BALF), attenuated neutrophil infiltration in the BALF and lung tissue, increased superoxide dismutase activity and glutathione levels, and alleviated myeloperoxidase expression in the lung tissue of mice with lung injury. Tomatidine also decreased inflammatory cytokine and chemokine gene expression in inflammatory lungs and attenuated the phosphorylation of mitogen-activated protein kinase and nuclear factor kappa B. Furthermore, tomatidine enhanced the production of heme oxygenase-1, decreased the secretion of inflammatory cytokines and chemokines in LPS-stimulated lung epithelial cells, and attenuated THP-1 monocyte adhesion. Our findings suggest that tomatidine attenuates oxidative stress and inflammation, improving acute lung injury in mice. |
| format | Article |
| id | doaj-art-f27aacba9b2442a2b152d3f52b7fc9d1 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-f27aacba9b2442a2b152d3f52b7fc9d12025-02-03T07:23:31ZengWileyMediators of Inflammation0962-93511466-18612021-01-01202110.1155/2021/45442944544294Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung InjuryWen-Chung Huang0Shu-Ju Wu1Ya-Ling Chen2Chwan-Fwu Lin3Chian-Jiun Liou4Graduate Institute of Health Industry Technology, Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Taoyuan City 33303, TaiwanDepartment of Nutrition and Health Sciences, Chang Gung University of Science and Technology, Taoyuan City 33303, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, TaiwanDepartment of Cosmetic Science, Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Taoyuan City 33303, TaiwanDivision of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Taoyuan City 33303, TaiwanTomatidine, which is isolated from green tomato, can ameliorate inflammation and oxidative stress in cells and animal experiments and has been shown to improve airway inflammation in a murine model of asthma. Here, we investigated whether tomatidine can ameliorate acute lung injury in mice. Mice were given tomatidine by intraperitoneal injection for 7 consecutive days, and then, lung injury was induced via intratracheal instillation of lipopolysaccharide (LPS). Tomatidine reduced inflammatory cytokine expressions in bronchoalveolar lavage fluid (BALF), attenuated neutrophil infiltration in the BALF and lung tissue, increased superoxide dismutase activity and glutathione levels, and alleviated myeloperoxidase expression in the lung tissue of mice with lung injury. Tomatidine also decreased inflammatory cytokine and chemokine gene expression in inflammatory lungs and attenuated the phosphorylation of mitogen-activated protein kinase and nuclear factor kappa B. Furthermore, tomatidine enhanced the production of heme oxygenase-1, decreased the secretion of inflammatory cytokines and chemokines in LPS-stimulated lung epithelial cells, and attenuated THP-1 monocyte adhesion. Our findings suggest that tomatidine attenuates oxidative stress and inflammation, improving acute lung injury in mice.http://dx.doi.org/10.1155/2021/4544294 |
| spellingShingle | Wen-Chung Huang Shu-Ju Wu Ya-Ling Chen Chwan-Fwu Lin Chian-Jiun Liou Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury Mediators of Inflammation |
| title | Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury |
| title_full | Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury |
| title_fullStr | Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury |
| title_full_unstemmed | Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury |
| title_short | Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury |
| title_sort | tomatidine improves pulmonary inflammation in mice with acute lung injury |
| url | http://dx.doi.org/10.1155/2021/4544294 |
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