Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample
Eighteen bacterial strains, isolated from a waste water sample collected from a chemically contaminated site, Patancheru (17°32′N 78°16′E/17.53°N 78.27°E), India, were able to decolorize 4-chloro-2-nitrophenol (4C2NP) in the presence of an additional carbon source. These eighteen 4C2NP-decolorizing...
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Wiley
2016-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/7589068 |
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| author | Pankaj Kumar Arora Alok Srivastava Vijay Pal Singh |
| author_facet | Pankaj Kumar Arora Alok Srivastava Vijay Pal Singh |
| author_sort | Pankaj Kumar Arora |
| collection | DOAJ |
| description | Eighteen bacterial strains, isolated from a waste water sample collected from a chemically contaminated site, Patancheru (17°32′N 78°16′E/17.53°N 78.27°E), India, were able to decolorize 4-chloro-2-nitrophenol (4C2NP) in the presence of an additional carbon source. These eighteen 4C2NP-decolorizing strains have been identified as members of four different genera, including Bacillus, Paenibacillus, Pseudomonas, and Leuconostoc based on the 16S rRNA gene sequencing and phylogenetic analysis. Most of the bacteria (10) belonged to the genus Bacillus and contributed 56% of the total 4C2NP-degrading bacteria, whereas the members of genera Paenibacillus and Pseudomonas represented 22% and 17%, respectively, of total 4C2NP-degrading isolates. There was only one species of Leuconostoc capable of degrading 4C2NP. This is the first report of the diversity of 4C2NP-decolorizing bacteria in a waste water sample. Furthermore, one bacterium, Bacillus aryabhattai strain PC-7, was able to decolorize 4C2NP up to a concentration of 2.0 mM. Gas chromatography-mass spectrometry analysis identified 5-chloro-2-methylbenzoxazole as the final product of 4C2NP decolorization in strain PC-7. |
| format | Article |
| id | doaj-art-f2788d2ba1704e36b659f2e3ca20c5bb |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | Journal of Chemistry |
| spelling | doaj-art-f2788d2ba1704e36b659f2e3ca20c5bb2025-02-03T07:26:02ZengWileyJournal of Chemistry2090-90632090-90712016-01-01201610.1155/2016/75890687589068Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water SamplePankaj Kumar Arora0Alok Srivastava1Vijay Pal Singh2School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of KoreaDepartment of Plant Science, Faculty of Applied Sciences, MJP Rohilkhand University, Bareilly, IndiaDepartment of Plant Science, Faculty of Applied Sciences, MJP Rohilkhand University, Bareilly, IndiaEighteen bacterial strains, isolated from a waste water sample collected from a chemically contaminated site, Patancheru (17°32′N 78°16′E/17.53°N 78.27°E), India, were able to decolorize 4-chloro-2-nitrophenol (4C2NP) in the presence of an additional carbon source. These eighteen 4C2NP-decolorizing strains have been identified as members of four different genera, including Bacillus, Paenibacillus, Pseudomonas, and Leuconostoc based on the 16S rRNA gene sequencing and phylogenetic analysis. Most of the bacteria (10) belonged to the genus Bacillus and contributed 56% of the total 4C2NP-degrading bacteria, whereas the members of genera Paenibacillus and Pseudomonas represented 22% and 17%, respectively, of total 4C2NP-degrading isolates. There was only one species of Leuconostoc capable of degrading 4C2NP. This is the first report of the diversity of 4C2NP-decolorizing bacteria in a waste water sample. Furthermore, one bacterium, Bacillus aryabhattai strain PC-7, was able to decolorize 4C2NP up to a concentration of 2.0 mM. Gas chromatography-mass spectrometry analysis identified 5-chloro-2-methylbenzoxazole as the final product of 4C2NP decolorization in strain PC-7.http://dx.doi.org/10.1155/2016/7589068 |
| spellingShingle | Pankaj Kumar Arora Alok Srivastava Vijay Pal Singh Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample Journal of Chemistry |
| title | Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample |
| title_full | Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample |
| title_fullStr | Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample |
| title_full_unstemmed | Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample |
| title_short | Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample |
| title_sort | diversity of 4 chloro 2 nitrophenol degrading bacteria in a waste water sample |
| url | http://dx.doi.org/10.1155/2016/7589068 |
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