Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cells

Objective: To investigate the effects of silkworm pupa (Bombyx mori) protein (SPP) on cell proliferation, apoptosis and energy metabolism in human colon cancer cells DLD-1. Methods: CCK-8 was used to detect cell proliferation rate after 72 h of cell culture for the control group (normal cultured DLD...

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Main Authors: Xiaojiao Ji, Jing Wang, Aijin Ma, Duo Feng, Yue He, Wenjie Yan
Format: Article
Language:English
Published: Tsinghua University Press 2022-09-01
Series:Food Science and Human Wellness
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Online Access:http://www.sciencedirect.com/science/article/pii/S2213453022000490
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author Xiaojiao Ji
Jing Wang
Aijin Ma
Duo Feng
Yue He
Wenjie Yan
author_facet Xiaojiao Ji
Jing Wang
Aijin Ma
Duo Feng
Yue He
Wenjie Yan
author_sort Xiaojiao Ji
collection DOAJ
description Objective: To investigate the effects of silkworm pupa (Bombyx mori) protein (SPP) on cell proliferation, apoptosis and energy metabolism in human colon cancer cells DLD-1. Methods: CCK-8 was used to detect cell proliferation rate after 72 h of cell culture for the control group (normal cultured DLD-1 cells) and SPP dose groups; Annexin-V/PI was applied to observe cell apoptosis; XFe24 Extracellular Flux Analyzer was used to detect cell mitochondrial respiratory function and glycolytic function. Results: Comparing with the control, SPP significantly inhibited the proliferation of DLD-1 cells with all the dosage tested (P < 0.01); flow cytometry showed that SPP significantly promoted apoptosis (P < 0.05). Additionally, SPP could significantly inhibited mitochondrial metabolism and glycolysis of DLD-1 cells and decreased cell energy metabolism in all groups treated with different doses. Conclusion: SPP can cause oxidative damage, promote apoptosis, and reduce mitochondrial respiratory and glycolysis rate in colon cancer DLD-1 cells, which reveals that SPP has the potential to serve as the anti-cancer drugs in the future, but further experimental evidence is needed.
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publisher Tsinghua University Press
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series Food Science and Human Wellness
spelling doaj-art-f2745ed36bb642758d7ab1b6b905ea4c2025-02-03T05:38:22ZengTsinghua University PressFood Science and Human Wellness2213-45302022-09-0111511711176Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cellsXiaojiao Ji0Jing Wang1Aijin Ma2Duo Feng3Yue He4Wenjie Yan5College of Biochemical Engineering, Beijing Union University, Beijing 100101, ChinaInstitute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing 100125, ChinaCollege of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing 100101, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing 100101, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing 100101, China; Corresponding author at: College of Biochemical Engineering, Beijing Union University, Beijing 100101, China.Objective: To investigate the effects of silkworm pupa (Bombyx mori) protein (SPP) on cell proliferation, apoptosis and energy metabolism in human colon cancer cells DLD-1. Methods: CCK-8 was used to detect cell proliferation rate after 72 h of cell culture for the control group (normal cultured DLD-1 cells) and SPP dose groups; Annexin-V/PI was applied to observe cell apoptosis; XFe24 Extracellular Flux Analyzer was used to detect cell mitochondrial respiratory function and glycolytic function. Results: Comparing with the control, SPP significantly inhibited the proliferation of DLD-1 cells with all the dosage tested (P < 0.01); flow cytometry showed that SPP significantly promoted apoptosis (P < 0.05). Additionally, SPP could significantly inhibited mitochondrial metabolism and glycolysis of DLD-1 cells and decreased cell energy metabolism in all groups treated with different doses. Conclusion: SPP can cause oxidative damage, promote apoptosis, and reduce mitochondrial respiratory and glycolysis rate in colon cancer DLD-1 cells, which reveals that SPP has the potential to serve as the anti-cancer drugs in the future, but further experimental evidence is needed.http://www.sciencedirect.com/science/article/pii/S2213453022000490colon cancer DLD-1 cellssilkworm pupa proteinapoptosismitochondriaglycolysis
spellingShingle Xiaojiao Ji
Jing Wang
Aijin Ma
Duo Feng
Yue He
Wenjie Yan
Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cells
Food Science and Human Wellness
colon cancer DLD-1 cells
silkworm pupa protein
apoptosis
mitochondria
glycolysis
title Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cells
title_full Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cells
title_fullStr Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cells
title_full_unstemmed Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cells
title_short Effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer DLD-1 cells
title_sort effects of silkworm pupa protein on apoptosis and energy metabolism in human colon cancer dld 1 cells
topic colon cancer DLD-1 cells
silkworm pupa protein
apoptosis
mitochondria
glycolysis
url http://www.sciencedirect.com/science/article/pii/S2213453022000490
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