Escherichia coli stress response systems and their reaction to terahertz radiation
In this review, we summarize the latest data concerning the reactions of Escherichia coli to nonthermal terahertz radiation and the underlying molecular mechanisms. E. coli is the most simple and convenient model object for studying the effects of terahertz radiation: both its genetics and metabolis...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2017-02-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/864 |
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| _version_ | 1832575267105669120 |
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| author | S. E. Peltek E. V. Demidova V. M. Popik T. N. Goryachkovskaya |
| author_facet | S. E. Peltek E. V. Demidova V. M. Popik T. N. Goryachkovskaya |
| author_sort | S. E. Peltek |
| collection | DOAJ |
| description | In this review, we summarize the latest data concerning the reactions of Escherichia coli to nonthermal terahertz radiation and the underlying molecular mechanisms. E. coli is the most simple and convenient model object for studying the effects of terahertz radiation: both its genetics and metabolism are well studied, and it is easily amenable to genetic engineering allowing one to create biosensors using promoters of genes activated by certain stress factors and the reporter GFP protein. Transformed E. coli cells containing biosensors can be used to visualize their reactions to terahertz radiation based on the intensity of GFP fluorescence. In this review, we present data on the response of certain E. сoli stress response systems to terahertz radiation obtained by us, as well as by other authors. We discuss experimental results for E. сoli/ pKatG-GFP, E. сoli/pCopA-GFP, and E. сoli/ pEmrR-GFP biosensors that are used to detect E. сoli genetic networks responding to oxidative stress, copper ion homeostasis failures, and antiseptics, respectively. The obtained data indicate that exposure to nonthermal terahertz radiation induces E. сoli gene networks of oxidative stress and copper ion homeostasis, but does not activate those responding to antibiotics, protonophores, or superoxide anions. The fact that E. сoli/pKatG-GFP and E. сoli/pCopA-GFP biosensors have different activation and reaction periods when exposed to terahertz radiation and natural inducers suggests that reactions of oxidative stress and copper ion homeostasis systems to terahertz radiation are specific. |
| format | Article |
| id | doaj-art-f2722c4c99aa427b942da0c74d0da775 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2017-02-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-f2722c4c99aa427b942da0c74d0da7752025-02-01T09:58:03ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592017-02-0120687688610.18699/VJ16.206553Escherichia coli stress response systems and their reaction to terahertz radiationS. E. Peltek0E. V. Demidova1V. M. Popik2T. N. Goryachkovskaya3Institute of Cytology and Genetics SB RASInstitute of Cytology and Genetics SB RASBudker Institute of Nuclear Physics SB RASInstitute of Cytology and Genetics SB RASIn this review, we summarize the latest data concerning the reactions of Escherichia coli to nonthermal terahertz radiation and the underlying molecular mechanisms. E. coli is the most simple and convenient model object for studying the effects of terahertz radiation: both its genetics and metabolism are well studied, and it is easily amenable to genetic engineering allowing one to create biosensors using promoters of genes activated by certain stress factors and the reporter GFP protein. Transformed E. coli cells containing biosensors can be used to visualize their reactions to terahertz radiation based on the intensity of GFP fluorescence. In this review, we present data on the response of certain E. сoli stress response systems to terahertz radiation obtained by us, as well as by other authors. We discuss experimental results for E. сoli/ pKatG-GFP, E. сoli/pCopA-GFP, and E. сoli/ pEmrR-GFP biosensors that are used to detect E. сoli genetic networks responding to oxidative stress, copper ion homeostasis failures, and antiseptics, respectively. The obtained data indicate that exposure to nonthermal terahertz radiation induces E. сoli gene networks of oxidative stress and copper ion homeostasis, but does not activate those responding to antibiotics, protonophores, or superoxide anions. The fact that E. сoli/pKatG-GFP and E. сoli/pCopA-GFP biosensors have different activation and reaction periods when exposed to terahertz radiation and natural inducers suggests that reactions of oxidative stress and copper ion homeostasis systems to terahertz radiation are specific.https://vavilov.elpub.ru/jour/article/view/864terahertz radiatione. сoli stress response systemsbiosensors |
| spellingShingle | S. E. Peltek E. V. Demidova V. M. Popik T. N. Goryachkovskaya Escherichia coli stress response systems and their reaction to terahertz radiation Вавиловский журнал генетики и селекции terahertz radiation e. сoli stress response systems biosensors |
| title | Escherichia coli stress response systems and their reaction to terahertz radiation |
| title_full | Escherichia coli stress response systems and their reaction to terahertz radiation |
| title_fullStr | Escherichia coli stress response systems and their reaction to terahertz radiation |
| title_full_unstemmed | Escherichia coli stress response systems and their reaction to terahertz radiation |
| title_short | Escherichia coli stress response systems and their reaction to terahertz radiation |
| title_sort | escherichia coli stress response systems and their reaction to terahertz radiation |
| topic | terahertz radiation e. сoli stress response systems biosensors |
| url | https://vavilov.elpub.ru/jour/article/view/864 |
| work_keys_str_mv | AT sepeltek escherichiacolistressresponsesystemsandtheirreactiontoterahertzradiation AT evdemidova escherichiacolistressresponsesystemsandtheirreactiontoterahertzradiation AT vmpopik escherichiacolistressresponsesystemsandtheirreactiontoterahertzradiation AT tngoryachkovskaya escherichiacolistressresponsesystemsandtheirreactiontoterahertzradiation |