Stem Cells from Human Exfoliated Deciduous Teeth Attenuate Trigeminal Neuralgia in Rats
Trigeminal neuralgia is an incurable progressive nervous system disease that can last for several months or years. Stem cells from human exfoliated deciduous teeth (SHED) are a candidate source for cell-based therapy. Owing to their neuroprotective and immunomodulatory effects, these neural crest ce...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/8819884 |
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| author | Xiaofeng Bai Xuedi Zhang Chun Wang Yao Liu Xuemei Liu Yu Fan Xia Zhang |
| author_facet | Xiaofeng Bai Xuedi Zhang Chun Wang Yao Liu Xuemei Liu Yu Fan Xia Zhang |
| author_sort | Xiaofeng Bai |
| collection | DOAJ |
| description | Trigeminal neuralgia is an incurable progressive nervous system disease that can last for several months or years. Stem cells from human exfoliated deciduous teeth (SHED) are a candidate source for cell-based therapy. Owing to their neuroprotective and immunomodulatory effects, these neural crest cells have potential roles in mediating chronic pain. In this study, we established a rat model of chronic constriction injury of the infraorbital nerve (CCI-ION) to evaluate the analgesic effect of SHED in neuropathic pain. The effects of local SHED transplantation on inflammatory cell infiltration in the trigeminal nerve were investigated based on hematoxylin and eosin staining. The levels of proinflammatory factors in the injured nerve and transient receptor potential vanilloid type 1 (TRPV1) expression in the trigeminal nerve and ganglion were quantified. The data showed that systemic or local injection of SHED attenuated the sensitivity of rats to mechanical stimuli after nerve injury, and this effect lasted throughout the observation period of 8 weeks. PKH26-labeled SHED were distributed to the ipsilateral trigeminal ganglions 24 and 72 hours after local injection. SHED transplantation at the lesion site led to reduced inflammatory cell infiltration and proinflammatory cytokine levels in the injured nerve and inhibited CCI-ION-induced upregulation of TRPV1 expression in the trigeminal nerve and ganglion in the early phase. Therefore, these results provide preclinical evidence that supports the use of SHED in the treatment of trigeminal neuralgia and potentially other chronic pain conditions. |
| format | Article |
| id | doaj-art-f204f9b10fd94f90bc445e833652cf60 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-f204f9b10fd94f90bc445e833652cf602025-02-03T01:24:31ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/88198848819884Stem Cells from Human Exfoliated Deciduous Teeth Attenuate Trigeminal Neuralgia in RatsXiaofeng Bai0Xuedi Zhang1Chun Wang2Yao Liu3Xuemei Liu4Yu Fan5Xia Zhang6Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, China Medical University, 117, N, Nanjing Street, Shenyang, ChinaLiaoning Provincial Key Laboratory of Oral Diseases, 117, N, Nanjing Street, Shenyang, ChinaLiaoning Provincial Key Laboratory of Oral Diseases, 117, N, Nanjing Street, Shenyang, ChinaLiaoning Provincial Key Laboratory of Oral Diseases, 117, N, Nanjing Street, Shenyang, ChinaLiaoning Provincial Key Laboratory of Oral Diseases, 117, N, Nanjing Street, Shenyang, ChinaLiaoning Provincial Key Laboratory of Oral Diseases, 117, N, Nanjing Street, Shenyang, ChinaLiaoning Provincial Key Laboratory of Oral Diseases, 117, N, Nanjing Street, Shenyang, ChinaTrigeminal neuralgia is an incurable progressive nervous system disease that can last for several months or years. Stem cells from human exfoliated deciduous teeth (SHED) are a candidate source for cell-based therapy. Owing to their neuroprotective and immunomodulatory effects, these neural crest cells have potential roles in mediating chronic pain. In this study, we established a rat model of chronic constriction injury of the infraorbital nerve (CCI-ION) to evaluate the analgesic effect of SHED in neuropathic pain. The effects of local SHED transplantation on inflammatory cell infiltration in the trigeminal nerve were investigated based on hematoxylin and eosin staining. The levels of proinflammatory factors in the injured nerve and transient receptor potential vanilloid type 1 (TRPV1) expression in the trigeminal nerve and ganglion were quantified. The data showed that systemic or local injection of SHED attenuated the sensitivity of rats to mechanical stimuli after nerve injury, and this effect lasted throughout the observation period of 8 weeks. PKH26-labeled SHED were distributed to the ipsilateral trigeminal ganglions 24 and 72 hours after local injection. SHED transplantation at the lesion site led to reduced inflammatory cell infiltration and proinflammatory cytokine levels in the injured nerve and inhibited CCI-ION-induced upregulation of TRPV1 expression in the trigeminal nerve and ganglion in the early phase. Therefore, these results provide preclinical evidence that supports the use of SHED in the treatment of trigeminal neuralgia and potentially other chronic pain conditions.http://dx.doi.org/10.1155/2021/8819884 |
| spellingShingle | Xiaofeng Bai Xuedi Zhang Chun Wang Yao Liu Xuemei Liu Yu Fan Xia Zhang Stem Cells from Human Exfoliated Deciduous Teeth Attenuate Trigeminal Neuralgia in Rats Stem Cells International |
| title | Stem Cells from Human Exfoliated Deciduous Teeth Attenuate Trigeminal Neuralgia in Rats |
| title_full | Stem Cells from Human Exfoliated Deciduous Teeth Attenuate Trigeminal Neuralgia in Rats |
| title_fullStr | Stem Cells from Human Exfoliated Deciduous Teeth Attenuate Trigeminal Neuralgia in Rats |
| title_full_unstemmed | Stem Cells from Human Exfoliated Deciduous Teeth Attenuate Trigeminal Neuralgia in Rats |
| title_short | Stem Cells from Human Exfoliated Deciduous Teeth Attenuate Trigeminal Neuralgia in Rats |
| title_sort | stem cells from human exfoliated deciduous teeth attenuate trigeminal neuralgia in rats |
| url | http://dx.doi.org/10.1155/2021/8819884 |
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