Analysis of active components from different ages of old Wuyi rock tea by gradient extraction

In this study, a comparative analysis of the extraction yields of flavonoids, polyphenols, and terpenes from aged Wuyi rock tea of different years in solvents such as petroleum ether, dichloromethane, ethyl acetate, n-butanol, and water was conducted, which provides a theoretical basis for the separ...

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Main Authors: Yan Lang, Qianli Ma, Rongping Chen, Dongcai Yang, Xiaomei Hu, Chuanhai Zhang, Chenxi Shi, Zhonglin Guo
Format: Article
Language:English
Published: AIP Publishing LLC 2025-02-01
Series:AIP Advances
Online Access:http://dx.doi.org/10.1063/5.0252943
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author Yan Lang
Qianli Ma
Rongping Chen
Dongcai Yang
Xiaomei Hu
Chuanhai Zhang
Chenxi Shi
Zhonglin Guo
author_facet Yan Lang
Qianli Ma
Rongping Chen
Dongcai Yang
Xiaomei Hu
Chuanhai Zhang
Chenxi Shi
Zhonglin Guo
author_sort Yan Lang
collection DOAJ
description In this study, a comparative analysis of the extraction yields of flavonoids, polyphenols, and terpenes from aged Wuyi rock tea of different years in solvents such as petroleum ether, dichloromethane, ethyl acetate, n-butanol, and water was conducted, which provides a theoretical basis for the separation and purification of active components. A 75% ethanol reflux extraction method was employed to prepare the ethanol extract, followed by extraction of the aqueous suspension using different polar organic solvents and deep eutectic solvents. The contents were determined by UV spectrophotometry. The results showed that the total extraction yields of flavonoids and polyphenols varied with the solvents, in the order of n-butanol, ethyl acetate, water, dichloromethane, and petroleum ether; for triterpenoids, the order was n-butanol, water, petroleum ether, ethyl acetate, and dichloromethane. N-butanol was identified as the optimal solvent. This study also detected a flavonoid compound, presumably glycyrrhizic acid, based on comparisons with existing literature and information. Currently, research and development on flavonoids, polyphenols, and triterpenoids in aged tea are underway, aiming to further explore the value and application potential of tea and contribute to the development of the tea industry.
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spelling doaj-art-f1adf89480bd4b8792b24eccf3cb7c762025-08-20T03:15:57ZengAIP Publishing LLCAIP Advances2158-32262025-02-01152025121025121-810.1063/5.0252943Analysis of active components from different ages of old Wuyi rock tea by gradient extractionYan Lang0Qianli Ma1Rongping Chen2Dongcai Yang3Xiaomei Hu4Chuanhai Zhang5Chenxi Shi6Zhonglin Guo7Department of Rehabilitation Therapy, Wuyi University, Wuyishan, Fujian Province 354301, ChinaCollege of Tourism, Wuyi University, Wuyishan City, Fujian Province 354301, ChinaWuyishan Xiangjiang Tea Industry Co., Ltd., Wuyishan City, Fujian Province 354300, ChinaDongcai Tea Industry Co., Ltd., Wuyishan City, Fujian Province 354301, ChinaWuyishan Xiangjiang Tea Industry Co., Ltd., Wuyishan City, Fujian Province 354300, ChinaWuyi University, Wuyishan City, Fujian Province 354301, ChinaShandong Polytechnic College, Jining City, Shandong Province 272067, ChinaBinzhou Medical University, Yantai City, Shandong Province 264000, ChinaIn this study, a comparative analysis of the extraction yields of flavonoids, polyphenols, and terpenes from aged Wuyi rock tea of different years in solvents such as petroleum ether, dichloromethane, ethyl acetate, n-butanol, and water was conducted, which provides a theoretical basis for the separation and purification of active components. A 75% ethanol reflux extraction method was employed to prepare the ethanol extract, followed by extraction of the aqueous suspension using different polar organic solvents and deep eutectic solvents. The contents were determined by UV spectrophotometry. The results showed that the total extraction yields of flavonoids and polyphenols varied with the solvents, in the order of n-butanol, ethyl acetate, water, dichloromethane, and petroleum ether; for triterpenoids, the order was n-butanol, water, petroleum ether, ethyl acetate, and dichloromethane. N-butanol was identified as the optimal solvent. This study also detected a flavonoid compound, presumably glycyrrhizic acid, based on comparisons with existing literature and information. Currently, research and development on flavonoids, polyphenols, and triterpenoids in aged tea are underway, aiming to further explore the value and application potential of tea and contribute to the development of the tea industry.http://dx.doi.org/10.1063/5.0252943
spellingShingle Yan Lang
Qianli Ma
Rongping Chen
Dongcai Yang
Xiaomei Hu
Chuanhai Zhang
Chenxi Shi
Zhonglin Guo
Analysis of active components from different ages of old Wuyi rock tea by gradient extraction
AIP Advances
title Analysis of active components from different ages of old Wuyi rock tea by gradient extraction
title_full Analysis of active components from different ages of old Wuyi rock tea by gradient extraction
title_fullStr Analysis of active components from different ages of old Wuyi rock tea by gradient extraction
title_full_unstemmed Analysis of active components from different ages of old Wuyi rock tea by gradient extraction
title_short Analysis of active components from different ages of old Wuyi rock tea by gradient extraction
title_sort analysis of active components from different ages of old wuyi rock tea by gradient extraction
url http://dx.doi.org/10.1063/5.0252943
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